{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T08:26:55Z","timestamp":1758961615061,"version":"3.41.2"},"reference-count":43,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Toxoplasmosis occurs worldwide causing economic losses to the animal production and problems to the public health. The study aimed to detect Toxoplasma gondii and Sarcocystis spp.in 141 meat products from commercial meat cuts of pork, beef, and kibbeh sold in commercial markets from Botucatu, SP, Brazil. Samples were bioassayed in mice to isolate the parasite, and the parasite DNA detected by PCR targeting the 529 base pairs repeat element region (PCR-529-bp). All samples resulted negative on bioassay, whereas PCR positive for 9 (6,38%), distributed as 5\/48 beef, 3\/49 pork, and 1\/44 kibbeh. PCR-positive were investigated for the the parasite genotype using multiplex-, nested-, and RFLP-PCR for 11 markers (SAG1, 5\u2019-3\u2019SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Complete genotype was determined on just one PCR-positive sample that matched MAS, TgCkBr89 and TgCkBr147 isolates already identified. In addition, nested- and RFLP-PCR targeting 18S rRNA was run for all PCR-positive samples and, the products, sequenced and aligned to the GenBank at NCBI website. Four samples showed 100% homology with T. gondii (GenBank #L37415.1), three with Sarcocystis hominis (GenBank #AF006471.1), two Sarcocystis cruzi (GenBank #AF176934.1), and one Sarcocystis hirsuta (GenBank #AF006469.1), indicating the circulation of T. gondii and Sarcocystis spp.<\/jats:p>","DOI":"10.1590\/s1984-29612021051","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:40:12Z","timestamp":1624927212000},"source":"Crossref","is-referenced-by-count":5,"title":["Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil"],"prefix":"10.1590","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-0762","authenticated-orcid":false,"given":"Helio","family":"Langoni","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Generoso","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00canio Yoshinori","family":"Hayasaka","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine Bott","family":"Mantovan","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedito Donizete","family":"Menozzi","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virg\u00ednia Bodel\u00e3o","family":"Richini-Pereira","sequence":"additional","affiliation":[{"name":"Instituto Adolfo Lutz,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9319-7516","authenticated-orcid":false,"given":"Rodrigo Costa da","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade do Oeste Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2021]]},"reference":[{"key":"ref1","series-title":"Zoonosis y enfermedades transmisibles communes al hombre y a los animals.","author":"Acha P","year":"2003","edition":"3rd"},{"key":"ref2","series-title":"Zoonosis.","first-page":"229","volume-title":"Toxoplasma gondii in meat and food safety implications \u2013 a review.","author":"Bayarri S","year":"2012"},{"issue":"7","key":"ref3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0180906","article-title":"Spatial and simultaneous representative seroprevalence of anti-Toxoplasma gondii antibodies in owners and their domiciled dogs in a major city of southern Brazil","volume":"12","author":"Benitez AN","year":"2017","journal-title":"PLoS One"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1590\/S0037-86821997000100005","article-title":"Surto de toxoplasmose aguda transmitida atrav\u00e9s da ingest\u00e3o de carne crua de gado ovino","volume":"30","author":"Bonametti AM","year":"1997","journal-title":"Rev Soc Bras Med Trop"},{"key":"ref5","series-title":"Patologia veterin\u00e1ria especial.","author":"Carlton WW","year":"2005","edition":"2nd"},{"issue":"1-3","key":"ref6","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.smallrumres.2005.12.023","article-title":"Virulence and molecular characterization of Toxoplasma gondii isolated from goats in Cear\u00e1, Brazil","volume":"69","author":"Cavalcante ACR","year":"2007","journal-title":"Small Rumin Res"},{"issue":"7254","key":"ref7","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1136\/bmj.321.7254.142","article-title":"Sources of toxoplasma infection in pregnant women: European multicentre case-control study. 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