{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:40:01Z","timestamp":1758127201929,"version":"3.41.2"},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the positivity of polymerase chain reaction (PCR) for the diagnosis of canine leishmaniasis in conjunctiva samples. DNA extraction was performed using SDS 20% and PCR was performed using 13A\/13B primers that amplify 120-bp of Leishmania kDNA. Of the 77 dogs analyzed, 50 (64.93%) had ocular changes: 25 (32.47%) dogs had periocular lesion, 41 (53.25%) dogs had purulent eye discharge, and 17 (22.08%) dogs had both signals. PCR was positive in 35 dogs (45.45%), and there was no significant difference between dogs with and without ocular signals (p=0.4074). PCR positivity was significant higher in dogs without periocular injury (p=0.0018). Conjunctive PCR, a less invasive, fast, and painless collection technique, is indicated to complement the diagnosis, especially in dogs without periocular injury, independent of the presence of purulent eye discharge.<\/jats:p>","DOI":"10.1590\/s1984-29612021061","type":"journal-article","created":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T13:04:52Z","timestamp":1625835892000},"source":"Crossref","is-referenced-by-count":6,"title":["Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs"],"prefix":"10.1590","volume":"30","author":[{"given":"Karen Ara\u00fajo","family":"Magalh\u00e3es","sequence":"first","affiliation":[{"name":"Universidade Federal da Grande Dourados,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamily Fagundes","family":"Pussi","sequence":"additional","affiliation":[{"name":"Universidade Federal da Grande Dourados,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9lton Krisman de","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Universidade Federal da Grande Dourados,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Barbosa do","family":"Carmo","sequence":"additional","affiliation":[{"name":"Centro de Controle de Zoonoses,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabete","family":"Friozi","sequence":"additional","affiliation":[{"name":"Centro de Controle de Zoonoses,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidiane Schultz","family":"Branquinho","sequence":"additional","affiliation":[{"name":"Universidade Federal da Grande Dourados,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0146-7123","authenticated-orcid":false,"given":"Manoel Sebasti\u00e3o da Costa","family":"Lima 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