{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T19:54:15Z","timestamp":1790452455284,"version":"4.1.0"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to investigate the level of exposure to Leishmania infection in stray dogs in an area of intense visceral leishmaniasis transmission in the state of Pernambuco, Brazil. Blood samples from 178 dogs were analyzed using serological and molecular assays: rapid immunochromatographic test (ICT), enzyme-linked immunosorbent assay (ELISA), immunofluorescence antibody test (IFAT), and conventional and quantitative polymerase chain reaction (cPCR and qPCR). Positivity values obtained with serological tests were 71.4% (127\/178), 70.2% (125\/178), and 50.6% (90\/178) using ICT, ELISA, and IFAT, respectively, with 38.8% (69\/178) of the dogs were simultaneously positive for all three tests. The positivity values obtained with cPCR and qPCR were 20.2% (36\/178) and 38.8% (69\/178), respectively, with 11.8% (21\/178) testing positive in both molecular assays. Overall, 87.1% (155\/178) were positive for anti-Leishmania spp. antibodies and\/or Leishmania spp. DNA. Positivity to one or more tests was statistically associated with lymphadenomegaly, skin lesions, lymphocytosis, anemia and hyperproteinemia. The results of this study revealed a high level of exposure to Leishmania in stray dogs in an area of intense human visceral leishmaniasis transmission, suggesting that dogs play a role as reservoirs in the transmission cycle of this zoonosis.<\/jats:p>","DOI":"10.1590\/s1984-29612021067","type":"journal-article","created":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T12:18:41Z","timestamp":1629116321000},"source":"Crossref","is-referenced-by-count":4,"title":["Comparison of serological and molecular tests to investigate Leishmania spp. infections in stray dogs from an area of intense visceral leishmaniasis transmission in Brazil"],"prefix":"10.1590","volume":"30","author":[{"given":"Anna Maria da Cruz Ferreira","family":"Evaristo","sequence":"first","affiliation":[{"name":"Universidade Federal do Vale do S\u00e3o Francisco,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreina de Carvalho","family":"Araujo","sequence":"additional","affiliation":[{"name":"Universidade Federal do Vale do S\u00e3o Francisco,  Brasil; Universidade Federal de Minas Gerais,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a Pereira","family":"da Costa","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila Gaud\u00eancio da Silva","family":"Sales","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Alexandre Menezes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Netherlands Hanseniasis Relief,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipe","family":"Dantas-Torres","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  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