{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:52:58Z","timestamp":1789771978972,"version":"4.0.1"},"reference-count":26,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Leishmaniases are zoonotic diseases caused by protozoa of the genus Leishmania. The disease has two clinical manifestations described in humans: visceral (VL) and cutaneous (CL) leishmaniasis. In Brazil, there has been an expansion of human VL. The participation of the dog as a reservoir of Leishmania infantum, the agent of VL, is important for the epidemiology of the disease since canine cases generally precede human cases. The present study aimed to evaluate the occurrence of Leishmania spp. infection in dogs in the municipality of Ji-Paran\u00e1 by PCR assays using blood samples. Leishmania DNA was detected in two of the 105 studied dogs. The PCR products were sequenced and confirmed that the two samples (1.90%) correspond to L. infantum. The dogs had allochthonous history. Therefore, the positive results found here should serve as a warning to public health agencies. This is because Ji-Paran\u00e1 is the third municipality to register cases of canine leishmaniasis (CanL) in Rond\u00f4nia state. Thus, reinforcing the importance of expanding studies on the epidemiology and surveillance of VL in the region.<\/jats:p>","DOI":"10.1590\/s1984-29612021085","type":"journal-article","created":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T12:13:41Z","timestamp":1638188021000},"source":"Crossref","is-referenced-by-count":1,"title":["The spread of visceral leishmaniasis in Brazil: the first canine cases described in Ji-Paran\u00e1, Rond\u00f4nia, Brazil"],"prefix":"10.1590","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0710-7655","authenticated-orcid":false,"given":"Aliny Pontes","family":"Almeida","sequence":"first","affiliation":[{"name":"Centro Universit\u00e1rio S\u00e3o Lucas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Marques","family":"Pereira J\u00fanior","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil; Instituto Nacional Epidemiologia da Amaz\u00f4nia Ocidental,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Frassinetti Medeiros de","family":"Paulo","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano Mendes Marchandeau","family":"Pinto","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio S\u00e3o Lucas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Bastos","family":"Boroviec","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio S\u00e3o Lucas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geysa Almeida","family":"Viana","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio S\u00e3o Lucas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mois\u00e9s Thiago de Souza","family":"Freitas","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio do Vale do Ipojuca,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Ben\u00edcio Neves","family":"Fuverki","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio S\u00e3o Lucas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo de Godoi Mattos","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto Nacional Epidemiologia da Amaz\u00f4nia Ocidental,  Brasil; Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jansen Fernandes","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil; Instituto Nacional Epidemiologia da Amaz\u00f4nia Ocidental,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2021]]},"reference":[{"issue":"1","key":"ref1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1590\/S1984-29612010000100015","article-title":"Seroprevalence of anti-Leishmania spp. antibodies in rural dogs from the city of Monte Negro, State of Rond\u00f4nia, Brazil","volume":"19","author":"Aguiar DM","year":"2010","journal-title":"Rev Bras Parasitol Vet"},{"issue":"6","key":"ref2","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1579\/0044-7447-34.6.462","article-title":"Soybean production and conversion of tropical forest in the Brazilian Amazon: the case of Vilhena, Rond\u00f4nia","volume":"34","author":"Brown JC","year":"2005","journal-title":"Ambio"},{"issue":"3&#8211;4","key":"ref3","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetpar.2007.07.007","article-title":"The role of dogs as reservoirs of Leishmania parasites, with emphasis on Leishmania (Leishmania) infantum and Leishmania (Viannia) braziliensis.","volume":"149","author":"Dantas-Torres F","year":"2007","journal-title":"Vet Parasitol"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1046\/j.1365-2915.1998.00104.x","article-title":"Incrimination of Lutzomyia cruzi as a vector of American visceral leishmaniasis","volume":"12","author":"dos Santos SO","year":"1998","journal-title":"Med Vet Entomol"},{"issue":"5","key":"ref5","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar RC","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"ref6","doi-asserted-by":"crossref","first-page":"961","DOI":"10.3855\/jidc.4310","article-title":"Diagnosis of leishmaniasis","volume":"8","author":"Elmahallawy EK","year":"2014","journal-title":"J Infect Dev Ctries"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1101\/gr.8.3.186","article-title":"Base-calling of automated sequencer traces using Phred. 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