{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T21:45:10Z","timestamp":1775079910500,"version":"3.50.1"},"reference-count":37,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Visceral leishmaniasis is a parasitic zoonosis that mainly affects poorest and most vulnerable populations, and domestic dogs are considered to be the main source of infection to the vector and therefore humans. However, several studies have investigated the role of other vertebrate hosts in the disease cycle. In this context, the aim of the present study was to conduct a survey of Leishmania infantum infection in donkeys and mules living in a semiarid region of Brazil. Whole blood sampled from 72 equids (65 donkeys and 7 mules) was used to perform molecular diagnosis using the real-time polymerase chain reaction (qPCR) technique. A total of 25% of the samples (18\/72) were positive through qPCR, but there were no significant differences between the species (donkeys or mules), sex (male or female) and abandonment situation of the animals (yes or no). Donkeys and mules living under semiarid conditions have high frequency of L. infantum infection. It is therefore worth assigning importance to these species in the epidemiological cycle of visceral leishmaniasis, either as potential reservoirs or just as an abundant food source for vectors.<\/jats:p>","DOI":"10.1590\/s1984-29612021095","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T15:38:08Z","timestamp":1639409888000},"source":"Crossref","is-referenced-by-count":2,"title":["Molecular detection of Leishmania infantum in donkeys and mules under semiarid conditions in Brazil"],"prefix":"10.1590","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3151-3680","authenticated-orcid":false,"given":"Cl\u00e9cio Henrique","family":"Limeira","sequence":"first","affiliation":[{"name":"Universidade Federal de Campina Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murilo Duarte de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto Federal do Sert\u00e3o Pernambucano,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Pessoa","family":"Ara\u00fajo J\u00fanior","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Dantas","family":"Malossi","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila Sabrina","family":"Ullmann","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Luana Cristiny Rodrigues","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de Campina Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio Santos de","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Universidade Federal de Campina Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clebert Jos\u00e9","family":"Alves","sequence":"additional","affiliation":[{"name":"Universidade Federal de Campina Grande,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2021]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1089\/vbz.2015.1826","article-title":"Low seroprevalence of Leishmania infantum and Toxoplasma gondii in the horse population in Israel","volume":"15","author":"Aharonson-Raz K","year":"2015","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0738-081X(96)00041-7","article-title":"Leishmaniasis reservoir and their significance in control","volume":"14","author":"Ashford RW","year":"1996","journal-title":"Clin Dermatol"},{"key":"ref3","first-page":"85","article-title":"What it takes to be a 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