{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:36:27Z","timestamp":1764084987562,"version":"3.41.2"},"reference-count":48,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Haemonchus contortus is a constraint to sheep production. Seeking to reduce the use of hosts and produce parasitic stages in large-scale, a 42-day in vitro culture protocol of H. contortus third-stage larvae was optimized using Dulbecco\u2019s modified Eagle\u2019s medium (DMEM). In cell-free culture, larvae were maintained at 39.6\u00b0C, in acidic media (pH 6.1) for 3 or 6 days with \u03944-dafachronic acid followed by DMEM pH 7.4 supplemented or not with Fildes\u2019 reagent. In DMEM pH 7.4 at 37\u00b0C, supplementation with Caco-2 cells was compared to Fildes. On Day 14, fourth-stage larvae (L4) development rates in acidic media supplemented (86.8-88.4%) or not (74.4-77.8%) with Fildes and in Caco-2 cell co-culture (92.6%) were similar, and superior to DMEM pH 7.4 with Fildes (0.0%). On Day 21, Caco-2 cell co-culture resulted in higher larvae differentiation (25.0%) and lower degeneration (13.9%) compared to acidic media (1.5-8.1% and 48.6-69.9%, respectively). This is the first report of prolonged in vitro culture of H. contortus larvae using commercial media in co-culture with Caco-2 cells. Although no progression to the adult stage, Caco-2 cell co-culture resulted in morphological differentiation of H. contortus L4 and larval viability for up to 28 days.<\/jats:p>","DOI":"10.1590\/s1984-29612023005","type":"journal-article","created":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T16:46:58Z","timestamp":1673628418000},"source":"Crossref","is-referenced-by-count":5,"title":["In vitro culture of parasitic stages of Haemonchus contortus"],"prefix":"10.1590","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0046-0050","authenticated-orcid":false,"given":"Simone Cristina M\u00e9o","family":"Niciura","sequence":"first","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro Pelegrine","family":"Minho","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"McIntyre","sequence":"additional","affiliation":[{"name":"University of Glasgow,  United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda Vieira","family":"Benavides","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sul,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cintia Hiromi","family":"Okino","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio Novita","family":"Esteves","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina de Souza","family":"Chagas","sequence":"additional","affiliation":[{"name":"Embrapa Pecu\u00e1ria Sudeste,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro Francisco Talamini do","family":"Amarante","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista \u201cJ\u00falio de Mesquita Filho\u201d,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2018.12.008","article-title":"Anthelmintic effect and tissue alterations induced in vitro by hydrolysable tannis on the adult stage of the gastrointestinal nematode Haemonchus contortus.","volume":"266","author":"Acevedo-Ram\u00edrez PMC","year":"2019","journal-title":"Vet Parasitol"},{"key":"ref2","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2020.109060","article-title":"Long spelling periods are required for pasture to become free of contamination by infective larvae of Haemonchus contortus in a humid subtropical 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