{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:01:13Z","timestamp":1791360073116,"version":"4.3.1"},"reference-count":51,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to determine the presence of deoxyribonucleic acid (DNA) from Toxoplasma gondii, Sarcocystis spp. and Neospora caninum, in tissues of wild boars slaughtered in southern Brazil. A total of 156 samples were collected from different organs of 25 wild boars, and DNA from at least one of the protozoa investigated was detected in 79 samples. To differentiate between infectious agents, restriction fragment length polymorphism was performed using the restriction enzymes DdeI and HpaII. For N. caninum, conventional PCR was performed with specific primers. The DNA of at least one of the studied pathogens was detected in each animal: 26.58% for T. gondii, 68.36% for Sarcocystis spp. and 5.06% for N. caninum. Coinfection between T. gondii and Sarcocystis spp. occurred in 14 animals, between T. gondii and N. caninum in only one male animal, between Sarcocystis spp. and N. caninum in a female, while co-infection with the three agents was equally observed in only one male animal. Considering the high frequency of detection and its zoonotic risk, especially T. gondii, it appears that wild boars can be potential sources of transmission of infectious agents and the adoption of monitoring measures in these populations should be prioritized.<\/jats:p>","DOI":"10.1590\/s1984-29612023048","type":"journal-article","created":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T11:30:36Z","timestamp":1691753436000},"source":"Crossref","is-referenced-by-count":5,"title":["Molecular detection of Toxoplasma gondii, Neospora caninum and Sarcocystis spp in tissues of Sus scrofa slaughtered in southern Brazil"],"prefix":"10.1590","volume":"32","author":[{"given":"Bibiana Rodrigues de","family":"Freitas","sequence":"first","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilneia da","family":"Rosa","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isac Junior","family":"Roman","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Casquero","family":"Cunha","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Trevisan","family":"Gressler","sequence":"additional","affiliation":[{"name":"Instituto Federal Farroupilha,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Felipetto","family":"Cargnelutti","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1351-9912","authenticated-orcid":false,"given":"Fernanda Silveira Fl\u00f4res","family":"Vogel","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"4527","DOI":"10.1007\/s00436-016-5244-6","article-title":"Human intestinal sarcocystosis in Iran: there but not seen","volume":"115","author":"Agholi M","year":"2016","journal-title":"Parasitol Res"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1590\/S1984-29612010000200002","article-title":"Prevalence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in swine from Northeastern Brazil","volume":"19","author":"Azevedo SS","year":"2010","journal-title":"Rev Bras Parasitol Vet"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1111\/mam.12015","article-title":"A review of wild boar Sus scrofa diet and factors affecting food selection in native and introduced ranges","volume":"44","author":"Ballari SA","year":"2014","journal-title":"Mammal Rev"},{"issue":"8","key":"ref4","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.3390\/ani11082227","article-title":"Seroprevalence of Toxoplasma gondii and Salmonella in Hunted Wild Boars from Two Different Regions in Switzerland","volume":"11","author":"Bassi AMG","year":"2021","journal-title":"Animals (Basel)"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"29","DOI":"10.4322\/rbpv.018e1005","article-title":"Pesquisa de anticorpos anti-Neospora caninum em bovinos leiteiros, c\u00e3es e trabalhadores rurais da regi\u00e3o Sudoeste do Estado de Mato Grosso","volume":"18","author":"Benetti AH","year":"2009","journal-title":"Rev Bras Parasitol Vet"},{"issue":"4","key":"ref6","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.7589\/2013-02-034","article-title":"Neospora caninum exposure in overlapping populations of coyotes (Canis latrans) and feral swine (Sus scrofa)","volume":"49","author":"Bevins S","year":"2013","journal-title":"J Wildl Dis"},{"key":"ref7","article-title":"Instru\u00e7\u00e3o Normativa 3, de 31 de janeiro de 2013. 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