{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:01:02Z","timestamp":1781845262949,"version":"3.54.5"},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to investigate the association between chronic Anaplasma marginale and Babesia spp. infection and hematological parameters of pregnant and non-pregnant taurine heifers. Blood samples from 94 females were collected on the first day (D-10) of timed artificial insemination (TAI) protocol and on pregnancy diagnosis (D+34). Hematological parameters were determined and compared between pregnant (PG) and non-pregnant (NPG) heifers, and within group at different sampling days. Real-time PCR (qPCR) was used to determine A. marginale and Babesia bovis infection, and for absolute quantification of Babesia spp. between PG and NPG groups. Correlation analysis was performed between the number of gDNA copies (CN) of Babesia spp. and hematological parameters. On D-10, mean hemoglobin concentration was higher for NPG, and hematocrit and total plasma protein were higher on D+34 for both groups. There was no difference in Babesia spp. CN between groups. In the first qPCR, all heifers were positive for A. marginale and B. bovis. Significant correlations were found between hemoglobin and erythrocyte and between hemoglobin and hematocrit (r = 0.8082 and r = 0.3009, respectively). Low levels of A. marginale and Babesia spp. did not affect hematological parameters of chronically infected pregnant and non-pregnant taurine heifers.<\/jats:p>","DOI":"10.1590\/s1984-29612023052","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T11:26:03Z","timestamp":1693567563000},"source":"Crossref","is-referenced-by-count":1,"title":["Association between chronic Anaplasma marginale and Babesia spp. infection and hematological parameters of taurine heifers"],"prefix":"10.1590","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1633-2355","authenticated-orcid":false,"given":"Natalia Machado","family":"Rahal","sequence":"first","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3334-4657","authenticated-orcid":false,"given":"Gabriela Bueno","family":"Luz","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2920-3456","authenticated-orcid":false,"given":"Kau\u00ea Rodriguez","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3715-2345","authenticated-orcid":false,"given":"Bernardo Garziera","family":"Gasperin","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0233-3939","authenticated-orcid":false,"given":"Josiane de Oliveira","family":"Feij\u00f3","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6983-659X","authenticated-orcid":false,"given":"Andr\u00e9 Gustavo Cabrera","family":"Dalto","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil; Universidade Federal do Rio Grande do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8257-3635","authenticated-orcid":false,"given":"Monique Tomazele","family":"Rovani","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil; Universidade Federal do Rio Grande do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0145-0321","authenticated-orcid":false,"given":"Rodrigo Casquero","family":"Cunha","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0855-2750","authenticated-orcid":false,"given":"Marcio Nunes","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Universidade 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JL","year":"2015","edition":"5"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":["Associa\u00e7\u00e3o entre infec\u00e7\u00e3o cr\u00f4nica por Anaplasma marginale e Babesia spp. e par\u00e2metros hematol\u00f3gicos de novilhas taurinas"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612023000300309&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T11:26:17Z","timestamp":1693567577000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612023000300309&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":29,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023]]}},"alternative-id":["S1984-29612023000300309"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612023052","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e006423"}}