{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T08:19:17Z","timestamp":1785226757627,"version":"3.55.0"},"reference-count":26,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The aim of this study was to identify Cryptosporidium species found in cattle and sheep in Paran\u00e1, southern region of Brazil. Individual fecal samples from 458 bovines and 101 sheep were submitted for molecular analysis by PCR and nested PCR using specific primers for sequences of the 18S ribosomal unit (rRNA). Positive samples were analyzed using restriction fragment length polymorphism (RFLP), followed by genetic sequencing for species confirmation. The occurrence of Cryptosporidium was 11.27% (63\/559). The highest occurrence was detected in lambs (12\/59, 20.33%). From the 63 positive samples, it was possible to identify the species in 58 of them by RFLP and genetic sequencing. Five species of Cryptosporidium were identified: Cryptosporidium andersoni, Cryptosporidium bovis, Cryptosporidium ryanae, Cryptosporidium xiaoi, and Cryptosporidium parvum. The most prevalent species was C. andersoni (41.38%) and the least predominant was C. parvum (10.34%). The most abundant species of Cryptosporidium in dairy calves were C. andersoni (11\/25) and C. ryanae (6\/25). Of the 17 positive sheep, nine (52.94%) were infected with C. andersoni. This finding is the first report on the occurrence of C. andersoni in naturally infected sheep in Brazil and the first observation of a high absolute occurrence of this Cryptosporidium species in sheep.<\/jats:p>","DOI":"10.1590\/s1984-29612023076","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T11:08:56Z","timestamp":1701688136000},"source":"Crossref","is-referenced-by-count":6,"title":["Molecular characterization of Cryptosporidium in ruminants and observation of natural infection by Cryptosporidium andersoni in sheep from Paran\u00e1, Brazil"],"prefix":"10.1590","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5630-3295","authenticated-orcid":false,"given":"Luciane","family":"Holsback","sequence":"first","affiliation":[{"name":"Universidade Estadual do Norte do Paran\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen de Souza","family":"Marquez","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte do Paran\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Alves da","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte do Paran\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr\u00f4nio Pinheiro","family":"Porto","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte do Paran\u00e1,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Luis","family":"Garcia","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felippe Danyel Cardoso","family":"Martins","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9rcia de","family":"Seixas","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Londrina,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2023]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1017\/S0031182022001196","article-title":"The global prevalence of Cryptosporidium in sheep: a systematic review and meta-analysis","volume":"149","author":"Chen Y","year":"2022","journal-title":"Parasitology"},{"issue":"3-4","key":"ref2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.vetpar.2008.10.096","article-title":"Prevalence and molecular characterization of Cryptosporidium spp. in dairy calves from 11 farms in Prince Edward Island, Canada","volume":"160","author":"Coklin T","year":"2009","journal-title":"Vet Parasitol"},{"issue":"3","key":"ref3","first-page":"100","article-title":"Ghaffari far F. Molecular study on Cryptosporidium andersoni strains isolated from sheep based on 18S rRNA Gene","volume":"3","author":"Dalimi A","year":"2017","journal-title":"Infect Epidemiol Microbiol"},{"issue":"1-2","key":"ref4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2006.10.001","article-title":"Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines","volume":"144","author":"Feng Y","year":"2007","journal-title":"Vet Parasitol"},{"issue":"11","key":"ref5","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.pt.2018.07.009","article-title":"Genetic diversity and population structure of Cryptosporidium.","volume":"34","author":"Feng Y","year":"2018","journal-title":"Trends Parasitol"},{"issue":"3-4","key":"ref6","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.vetpar.2010.10.036","article-title":"Molecular characterization of Cryptosporidium in Brazilian sheep","volume":"175","author":"Fiuza VRS","year":"2011","journal-title":"Vet Parasitol"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1590\/S1984-29612016025","article-title":"Anthelmintic efficiency of doramectin, fenbendazole, and nitroxynil, in combination or individually, in sheep worm control","volume":"25","author":"Holsback L","year":"2016","journal-title":"Rev Bras Parasitol Vet"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.gtc.2006.03.006","article-title":"An updated review on Cryptosporidium and Giardia.","volume":"35","author":"Huang DB","year":"2006","journal-title":"Gastroenterol Clin North Am"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.exppara.2014.03.021","article-title":"Cryptosporidium species in sheep and goats from Papua New Guinea","volume":"141","author":"Koinari M","year":"2014","journal-title":"Exp Parasitol"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"373","DOI":"10.14411\/fp.2004.047","article-title":"Failed attempt of Cryptosporidium andersoni infection in lambs","volume":"51","author":"Kv\u00e1c M","year":"2004","journal-title":"Folia Parasitol"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1550-7408.2000.tb00016.x","article-title":"Cryptosporidium andersoni sp. (Apicomplexa: Cryptosporidiidae) from cattle, Bos taurus.","volume":"47","author":"Lindsay DS","year":"2000","journal-title":"J Eukaryot Microbiol"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1016\/S0016-5085(76)80331-9","article-title":"Overwhelming watery diarrhea associated with a Cryptosporidium in an immunosuppressed patient","volume":"70","author":"Meisel JL","year":"1976","journal-title":"Gastroenterology"},{"issue":"1-2","key":"ref13","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetpar.2009.07.033","article-title":"Molecular characterization of Cryptosporidium spp. from fecal samples of birds kept in captivity in Brazil","volume":"166","author":"Nakamura AA","year":"2009","journal-title":"Vet Parasitol"},{"issue":"4","key":"ref14","doi-asserted-by":"crossref","DOI":"10.1590\/s1984-29612021094","article-title":"Identification, molecular characterization and factors associate with occurrences of Cryptosporidium spp. in calves on dairy farms in Brazil","volume":"30","author":"Oliveira JS","year":"2021","journal-title":"Rev Bras Parasitol Vet"},{"issue":"1","key":"ref15","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1590\/S1984-29612013005000010","article-title":"Identification of Cryptosporidium species and genotypes in dairy cattle in Brazil","volume":"22","author":"Paz e Silva FM","year":"2013","journal-title":"Rev Bras Parasitol Vet"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"193","DOI":"10.2478\/s11686-014-0223-5","article-title":"High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil","volume":"59","author":"Paz e Silva FM","year":"2014","journal-title":"Acta Parasitol"},{"issue":"19","key":"ref17","doi-asserted-by":"crossref","first-page":"6026","DOI":"10.1128\/AEM.00606-08","article-title":"Cryptosporidium genotypes and subtypes in lambs and goat kids in Spain","volume":"74","author":"Qu\u00edlez J","year":"2008","journal-title":"Appl Environ Microbiol"},{"issue":"11","key":"ref18","doi-asserted-by":"crossref","first-page":"3307","DOI":"10.3390\/ani11113307","article-title":"An update on zoonotic Cryptosporidium species and genotypes in humans","volume":"11","author":"Ryan U","year":"2021","journal-title":"Animals"},{"issue":"9","key":"ref19","doi-asserted-by":"crossref","first-page":"4992","DOI":"10.1128\/AEM.71.9.4992-4997.2005","article-title":"Sheep may not be an important zoonotic reservoir for Cryptosporidium and Giardia parasites","volume":"71","author":"Ryan UM","year":"2005","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"ref20","first-page":"1917","article-title":"Occurrence and molecular characterization of Cryptosporidium sp. in sheep","volume":"38","author":"Snak A","year":"2017","journal-title":"Semina: Ci\u00eanc Agr\u00e1r"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175311","article-title":"Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms","volume":"12","author":"Toledo RS","year":"2017","journal-title":"PLoS One"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00436-009-1664-x","article-title":"Cervine genotype is the major Cryptosporidium genotype in sheep in China","volume":"106","author":"Wang Y","year":"2010","journal-title":"Parasitol Res"},{"key":"ref23","series-title":"Lista de mesorregi\u00f5es e microrregi\u00f5es do Paran\u00e1","year":"2022"},{"issue":"4","key":"ref24","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1128\/AEM.65.4.1578-1583.1999","article-title":"Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus","volume":"65","author":"Xiao L","year":"1999","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1128\/CMR.17.1.72-97.2004","article-title":"Cryptosporidium taxonomy: recent advances and implications for public health","volume":"17","author":"Xiao L","year":"2004","journal-title":"Clin Microbiol Rev"},{"issue":"1-2","key":"ref26","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.vetpar.2013.11.014","article-title":"Longitudinal prevalence, oocyst shedding and molecular characterisation of Cryptosporidium species in sheep across four states in Australia","volume":"200","author":"Yang R","year":"2014","journal-title":"Vet Parasitol"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":["Caracteriza\u00e7\u00e3o molecular de Cryptosporidium em ruminantes e observa\u00e7\u00e3o de infec\u00e7\u00e3o natural por Cryptosporidium andersoni em ovinos do Paran\u00e1, Brasil"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612023000400404&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T11:09:09Z","timestamp":1701688149000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612023000400404&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":26,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023]]}},"alternative-id":["S1984-29612023000400404"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612023076","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2023]]},"article-number":"e010023"}}