{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:45:37Z","timestamp":1765233937340,"version":"3.41.2"},"reference-count":32,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The tucunar\u00e9 (Cichla sp.) is an Amazonian fish that is heavily commercialized in the state of Amap\u00e1, and it can be infected by a variety of parasites, including coccidia of the genus Calyptospora, which are identified at the genus level by analyzing the structures that comprise its morphology. This study aimed to describe the morphology and histopathology of Calyptospora sp. parasitism in Cichla monoculus Spix, 1929 in the Municipality of Pracu\u00faba, Amap\u00e1, Brazil. Nine specimens were acquired from the Lake Sacaizal by artisanal fishermen and transported in isothermal boxes to the Integrated Morpho-molecular and Technologies Laboratory (LIMT) of the Federal Rural University of the Amazon in Bel\u00e9m, Par\u00e1, where they were necropsied. Fragments of the liver were removed to visualize cysts using light microscopy and processed for scanning electron microscopy and histology analyses. The analysis revealed that 66.6% of the fish examined had clusters of oocysts in the hepatic region, resulting in the formation of melanomacrophagic centers. The oocysts were sphere-like, with a diameter of 21 \u00b5m. They contained four pyriform sporocysts, 8.7 \u00b5m long and 4.9 \u00b5m wide, with sporopods in the posterior region.<\/jats:p>","DOI":"10.1590\/s1984-29612024078","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T11:10:31Z","timestamp":1734693031000},"source":"Crossref","is-referenced-by-count":1,"title":["Morphological and histopathological description of Calyptospora sp. parasitism in Cichla monoculus Spix, 1929 (Osteichthyes, Cichlidae) from the lake region of Pracu\u00faba-Amap\u00e1, Brazil"],"prefix":"10.1590","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3201-2459","authenticated-orcid":false,"given":"Eloiza Sarmento","family":"Amoras","sequence":"first","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5642-5361","authenticated-orcid":false,"given":"Jhonata","family":"Eduard","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1,  Brasil; Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria do Perp\u00e9tuo Socorro","family":"Progene","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Francisco Berr\u00eado Reis da","family":"Silva","sequence":"additional","affiliation":[{"name":"Museu Paraense Em\u00edlio Goeldi,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9559-8521","authenticated-orcid":false,"given":"Marcela Nunes","family":"Videira","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amap\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8784-0891","authenticated-orcid":false,"given":"Jos\u00e9 Ledamir","family":"Sindeaux-Neto","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil; Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2525-423X","authenticated-orcid":false,"given":"Michele","family":"Velasco","sequence":"additional","affiliation":[{"name":"Universidade Federal Rural da Amaz\u00f4nia,  Brasil; Universidade Federal Rural da Amaz\u00f4nia,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref1","series-title":"Integridade ambiental da represa de Jurumirim: ictiofauna e rela\u00e7\u00f5es ecol\u00f3gicas.","first-page":"115","volume-title":"Aspectos parasitol\u00f3gicos dos peixes.","author":"Acosta AA","year":"2016"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.ejop.2009.12.005","article-title":"First report of Calyptospora sp. (Apicomplexa, Calyptosporidae) in forage characid fish from the Tr\u00eas Marias Reservoir, S\u00e3o Francisco Basin, Brazil","volume":"46","author":"Albuquerque MC","year":"2010","journal-title":"Eur J Protistol"},{"issue":"6","key":"ref3","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/j.1365-2761.1995.tb00351.x","article-title":"Ultrastructural data on sporogony of the coccidian parasite Calyptospora spinosa from the liver of the Amazonian fish, Crenicichla lepidota Heckel","volume":"18","author":"Azevedo C","year":"1995","journal-title":"J Fish Dis"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/BF00017665","article-title":"Calyptospora tucunarensis n. sp. (Apicomplexa: Sporozoea) from the liver of tucunare Cichla ocellaris in Brazil","volume":"18","author":"B\u00e9k\u00e9si L","year":"1991","journal-title":"Syst Parasitol"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S1984-29612014001","article-title":"Studies on coccidian oocysts (Apicomplexa: eucoccidiorida)","volume":"23","author":"Berto BP","year":"2014","journal-title":"Rev Bras Parasitol Vet"},{"issue":"1-2","key":"ref6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3354\/dao070081","article-title":"Hepatic Calyptospora sp. (Apicomplexa) infection in a wild-born, aquarium-held clutch of juvenile arapaima Arapaima gigas (Osteoglossidae)","volume":"70","author":"Bonar CJ","year":"2006","journal-title":"Dis Aquat Organ"},{"year":"2016","series-title":"Parasitos de peixes marinhos da Am\u00e9rica do Sul.","author":"Eiras JC","key":"ref7"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1111\/j.1550-7408.1985.tb04058.x","article-title":"Calyptospora empristica n. sp. (Eimeriorina: Calyptosporidae) from the liver of the starhead topminnow, Fundulus notti.","volume":"32","author":"Fournie JW","year":"1985","journal-title":"J Protozool"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"720","DOI":"10.2307\/3283611","article-title":"Host Specificity of Calyptospora funduli (Apicomplexa: Calyptosporidae) in Atheriniform Fishes","volume":"79","author":"Fournie JW","year":"1993","journal-title":"J Parasitol"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1645\/0022-3395(2000)086[0501:LCOCFA]2.0.CO;2","article-title":"Life cycle of Calyptospora funduli (Apicomplexa: calyptosporidae)","volume":"86","author":"Fournie JW","year":"2000","journal-title":"J Parasitol"},{"issue":"1","key":"ref11","first-page":"4","article-title":"Past: paleontological statistics software package for education and data analysis","volume":"4","author":"Hammer \u00d8","year":"2001","journal-title":"Palaeontol Electronica"},{"issue":"4","key":"ref12","first-page":"1078","article-title":"Cichlid fishes of the Amazon River drainage of Peru","volume":"4","author":"Kullander S","year":"1987","journal-title":"Copeia"},{"issue":"4","key":"ref13","first-page":"289","article-title":"A review of the South American cichlid genus Cichla, with descriptions of nine new species (Teleostei: cichlidae)","volume":"17","author":"Kullander SO","year":"2006","journal-title":"Ichthyol Explor Freshwat"},{"key":"ref14","series-title":"Checklist of the Freshwater Fishes of South America.","first-page":"605","volume-title":"Family Cichlidae.","author":"Kullander SO","year":"2003"},{"year":"1968","series-title":"Manual of histologic staining methods of the Armed Forces Institute of Pathology.","author":"Luna LG","key":"ref15"},{"issue":"4","key":"ref16","first-page":"575","article-title":"Ultraestrutura de protozo\u00e1rios parasitas de peixes da regi\u00e3o amaz\u00f4nica","volume":"29","author":"Matos E","year":"1999","journal-title":"Acta"},{"key":"ref17","article-title":"Prevalence and molecular characterisation of Calyptospora parasites Overstreet, Hawkins and Fourni\u00e9, 1984 (Apicomplexa: Calyptosporidae) in fishes from the Eastern Amazon, Brazil","volume":"73","author":"Negr\u00e3o MCS","year":"2019","journal-title":"Parasitol Int"},{"issue":"1","key":"ref18","first-page":"60","article-title":"Dieta do tucunar\u00e9 Cichla monoculus Agassiz, 1831- a\u00e7ude Thomaz Osterne de Alencar semi\u00e1rido cearense","volume":"16","author":"Neto CF","year":"2017","journal-title":"Cad Cult Cien"},{"issue":"1","key":"ref19","article-title":"Hepatic coccidiosis in Serrasalmus rhombeus Linnaeus, 1766 from the Amazon basin: morphological and histopathological aspects","volume":"30","author":"Oliveira JEF","year":"2021","journal-title":"Rev Bras Parasitol Vet"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1590\/1809-43922002324724","article-title":"A dieta e o consumo di\u00e1rio de alimento de Cichla monoculus na Amaz\u00f4nia central","volume":"32","author":"Rabelo H","year":"2002","journal-title":"Acta Amazon"},{"issue":"3","key":"ref21","first-page":"63","article-title":"Coccidiose hep\u00e1tica em Aspistor quadriscutis, Valenciennes, 1840 (Teleostei: Ariidae) capturados no Estu\u00e1rio do nordeste paraense, Amaz\u00f4nia, Brasil","volume":"23","author":"Ramos ABMA","year":"2018","journal-title":"Arch Vet Sci"},{"issue":"6","key":"ref22","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1007\/s00436-011-2770-0","article-title":"Morphology and histopathology of Calyptospora sp. (Apicomplexa: Calyptosporidae) in speckled peacock bass, Cichla temensis Humboldt, 1821 (Perciformes: Cichlidae), from the Maraj\u00f3-A\u00e7u River, Maraj\u00f3 Island, Brazil","volume":"110","author":"Santiago H","year":"2012","journal-title":"Parasitol Res"},{"issue":"7","key":"ref23","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider CA","year":"2012","journal-title":"Nat Methods"},{"issue":"1","key":"ref24","first-page":"43","article-title":"A pesca artesanal no estado do Amap\u00e1: estado atual e desafios","volume":"10","author":"Silva LMA","year":"2010","journal-title":"Bol Tec-Cient CEPNOR"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1590\/S1984-29612012000200020","article-title":"Calyptospora sp in Brachyplatystoma vaillantii trapped at the Vigia, State of Par\u00e1, Brazil","volume":"21","author":"Silva MC","year":"2012","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/jeu.12785","article-title":"Hepatic Coccidiosis in Triportheus angulatus Spix & Agassiz, 1829 (Characiformes: Triportheidae), a Tropical Fish from the Eastern Brazilian Amazon, with the Description of a New Species of Calyptospora (Apicomplexa: Calyptosporidae)","volume":"67","author":"Silva MF","year":"2020","journal-title":"J Eukaryot Microbiol"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1111\/jeu.12707","article-title":"Morphology and Phylogeny of Calyptospora paranaidji n. sp. (Eimeriorina: Calyptosporidae), an Apicomplexan Parasite of the Hepatic Tissue of Cichla piquiti Kullander & Ferreira, 2006, From a Reservoir in the Brazilian Amazon Region","volume":"66","author":"Silva MF","year":"2019","journal-title":"J Eukaryot Microbiol"},{"issue":"3","key":"ref28","first-page":"231","article-title":"Morfologia e nova ocorr\u00eancia de um cocc\u00eddio hepatopancre\u00e1tico parasita de peixe amaz\u00f4nico","volume":"55","author":"Velasco M","year":"2012","journal-title":"Rev Cienc Agrar (Belem)"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1590\/S0102-09352013000100014","article-title":"An anatomopathological study of hepatic coccidiosis (Calyptospora sp.) in the Acar\u00e1-pixuna, Aequidens plagiozonatus Kullander, 1984 from the Brazilian state of Par\u00e1","volume":"65","author":"Videira M","year":"2013","journal-title":"Arq Bras Med Vet Zootec"},{"key":"ref30","series-title":"Handbook of the Protists.","first-page":"1","volume-title":"Apicomplexa.","author":"Vot\u00fdpka J","year":"2016","edition":"2nd"},{"issue":"3","key":"ref31","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1007\/s00436-012-2969-8","article-title":"Phylogeny of fish- infecting Calyptospora species (Apicomplexa: eimeriorina)","volume":"111","author":"Whipps CM","year":"2012","journal-title":"Parasitol Res"},{"issue":"1","key":"ref32","first-page":"85","article-title":"A pesca artesanal na regi\u00e3o dos lagos: o caso do munic\u00edpio de Pracu\u00faba, Amap\u00e1","volume":"08","author":"Zacardi DM","year":"2021","journal-title":"Rev Desafios"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":["Descri\u00e7\u00e3o morfol\u00f3gica e histopatol\u00f3gica do parasitismo por Calyptospora sp. em Cichla monoculus Spix, 1929 (Osteichthyes, Cichlidae) oriundos da regi\u00e3o dos lagos de Pracu\u00faba-Amap\u00e1, Brasil"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612024000400408&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T11:10:40Z","timestamp":1734693040000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612024000400408&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":32,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024]]}},"alternative-id":["S1984-29612024000400408"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612024078","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"type":"electronic","value":"1984-2961"},{"type":"print","value":"0103-846X"}],"subject":[],"published":{"date-parts":[[2024]]},"article-number":"e012324"}}