{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:16:00Z","timestamp":1753888560866,"version":"3.41.2"},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Opossums are synanthropic animals that participate in the zoonotic transmission cycles. Chagas disease, a neglected tropical disease caused by Trypanosoma cruzi, affects many domestic and wild animals and humans worldwide. This study aimed to determine the occurrence of T. cruzi in free-ranging opossums in Canoinhas, Santa Catarina, Brazil. Fifty opossums (Didelphis albiventris) (33 captured and 17 road-killed) were evaluated using Nested-PCR assay. All tissue samples were negative (0\/17). Eight of the 33 (24.24%; 95% CI:11.94\u201340,89%) blood samples were positive for T. cruzi. No significant associations were found between the sex (male\/ female, p = 0.423), the trap area (rural\/urban, p = 0.163), and positivity for T. cruzi in opossum blood samples. All samples showed 100% identity with T. cruzi (KF788250) isolated from Panstrongylus megistus in S\u00e3o Paulo, Brazil. The phylogenetic analysis model allocated all sequences obtained from D. albiventris to the large TcI clade of T. cruzi. This study provides the first record of T. cruzi in white-eared opossums in Canoinhas, Santa Catarina, southern Brazil.<\/jats:p>","DOI":"10.1590\/s1984-29612025003","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T11:30:00Z","timestamp":1738582200000},"source":"Crossref","is-referenced-by-count":0,"title":["Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil"],"prefix":"10.1590","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8449-1083","authenticated-orcid":false,"given":"Giane Helenita","family":"Pontarolo","sequence":"first","affiliation":[{"name":"Universidade Federal do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6283-4981","authenticated-orcid":false,"given":"Daniela","family":"Pedrassani","sequence":"additional","affiliation":[{"name":"Universidade do Contestado,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds Felipe","family":"K\u00fchl","sequence":"additional","affiliation":[{"name":"Universidade do Contestado,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0082-0362","authenticated-orcid":false,"given":"Monique Paiva","family":"Campos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2368-7509","authenticated-orcid":false,"given":"Thais Cristina","family":"Tirado","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6861-0997","authenticated-orcid":false,"given":"Fabiano Borges","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8631-2712","authenticated-orcid":false,"given":"Th\u00e1llitha Samih Wischral Jayme","family":"Vieira","sequence":"additional","affiliation":[{"name":"The University of North Carolina at Charlotte,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4096-5937","authenticated-orcid":false,"given":"Ana Cl\u00e1udia","family":"Calchi","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6613-0287","authenticated-orcid":false,"given":"Rafael Felipe da Costa","family":"Vieira","sequence":"additional","affiliation":[{"name":"The University of North Carolina at Charlotte,  USA; The University of North Carolina at Charlotte,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-9358","authenticated-orcid":false,"given":"Ivan Roque de","family":"Barros Filho","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul SF","year":"1990","journal-title":"J Mol Biol"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.actatropica.2012.06.010","article-title":"The sylvatic transmission cycle of Trypanosoma cruzi in a rural area in the humid Chaco of Argentina","volume":"124","author":"Alvarado-Otegui JA","year":"2012","journal-title":"Acta Trop"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/nar\/30.1.17","article-title":"GenBank","volume":"30","author":"Benson DA","year":"2002","journal-title":"Nucleic Acids Res"},{"issue":"9","key":"ref4","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1128\/JCM.41.9.4172-4177.2003","article-title":"Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples","volume":"41","author":"Birkenheuer AJ","year":"2003","journal-title":"J Clin Microbiol"},{"edition":"4th","year":"2019","series-title":"Guia de Vigil\u00e2ncia em Sa\u00fade: volume \u00fanico.","key":"ref5"},{"year":"2021","series-title":"Doen\u00e7a de chagas","key":"ref6"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1101\/gr.8.3.186","article-title":"Base-calling of automated sequencer traces using Phred. 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