{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T10:25:04Z","timestamp":1768645504816,"version":"3.49.0"},"reference-count":33,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005667","name":"FAPESC","doi-asserted-by":"crossref","award":["2015TR1543"],"award-info":[{"award-number":["2015TR1543"]}],"id":[{"id":"10.13039\/501100005667","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005667","name":"FAPESC","doi-asserted-by":"crossref","award":["FAPESC1827\/2015"],"award-info":[{"award-number":["FAPESC1827\/2015"]}],"id":[{"id":"10.13039\/501100005667","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005667","name":"FAPESC","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100005667","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["2015TR1543"],"award-info":[{"award-number":["2015TR1543"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["FAPESC1827\/2015"],"award-info":[{"award-number":["FAPESC1827\/2015"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The objective of this study was to determine the prevalence of Babesia bigemina infection and associated risk factors in Crioula Lageana cattle, a Brazilian native breed known for its tick resistance. Blood samples were collected from 311 registered cattle (62 males, 249 females) from conservation nucleus properties in Santa Catarina State, Brazil. Samples underwent DNA extraction and nested PCR targeting the rap-1a gene for B. bigemina detection. Animals were categorized by sex, age class, and tick presence during sampling. An epidemiological questionnaire assessed potential risk factors. The overall B. bigemina prevalence was 60% (186\/311; 95% CI: 56,95%-62,67%). Males showed significantly higher infection rates (79%; 49\/62) compared to females (55%; 137\/249; OR=3.36, p&lt;0.001). Bulls (81%; 26\/32) and calves (78%; 56\/72) exhibited higher infection rates than cows (50%; 70\/141) and heifers (52%; 34\/66; p&lt;0.001). Tick presence during sampling increased infection probability (OR=2.00, p=0.006). Contact with other animal species (OR=1.57, p=0.037) and regular veterinary care (OR=6.77, p=0.009) were identified as significant risk factors. Results indicate enzootic instability in the studied population, with distinct sex-based susceptibility patterns. These findings provide baseline data for developing targeted control strategies for B. bigemina in Crioula Lageana breeding programs.<\/jats:p>","DOI":"10.1590\/s1984-29612025018","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T21:26:52Z","timestamp":1744061212000},"source":"Crossref","is-referenced-by-count":1,"title":["Prevalence and factors associated with Babesia bigemina infection in Crioula Lageana cattle breed"],"prefix":"10.1590","volume":"34","author":[{"given":"Mariana da Silva","family":"Casa","sequence":"first","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio de Mattos","family":"Vettori","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ketriane Mota","family":"de Souza","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Ricardo Benetti","family":"Todeschini","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Cl\u00e1udio","family":"Miletti","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil; Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Ivane Ganz","family":"Vogel","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil; Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Lu\u00eds Ferreira","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1194-6048","authenticated-orcid":false,"given":"Joandes Henrique","family":"Fonteque","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil; Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.ttbdis.2013.11.002","article-title":"Quantitative study of Babesia bovis infection in beef cattle from S\u00e3o Paulo state, Brazil","volume":"5","author":"Bilhassi TB","year":"2014","journal-title":"Ticks Tick Borne Dis"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"S247","DOI":"10.1017\/S0031182004005190","article-title":"Babesiosis of cattle","volume":"129","author":"Bock R","year":"2004","journal-title":"Parasitology"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1111\/j.1751-0813.1997.tb15706.x","article-title":"Effect of breed of cattle on innate resistance to infection with Babesia bovis, B. bigemina and Anaplasma marginale.","volume":"75","author":"Bock RE","year":"1997","journal-title":"Aust Vet J"},{"issue":"7","key":"ref4","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/j.1751-0813.1999.tb12093.x","article-title":"Effect of breed of cattle on transmission rate and innate resistance to infection with Babesia bovis and B bigemina transmitted by Boophilus microplus.","volume":"77","author":"Bock RE","year":"1999","journal-title":"Aust Vet J"},{"issue":"1-2","key":"ref5","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.vetpar.2007.03.031","article-title":"A quantitative PCR assay for the detection and quantification of Babesia bovis and B. bigemina.","volume":"147","author":"Buling A","year":"2007","journal-title":"Vet Parasitol"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"507","DOI":"10.3347\/kjp.2014.52.5.507","article-title":"First Evaluation of an Outbreak of Bovine Babesiosis and Anaplasmosis in Southern Brazil Using Multiplex PCR","volume":"52","author":"Canever MF","year":"2014","journal-title":"Korean J Parasitol"},{"issue":"1-3","key":"ref7","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.vetpar.2012.10.018","article-title":"Resistance against gastrointestinal nematodes in Crioulo Lageano and crossbred Angus cattle in southern Brazil","volume":"192","author":"Cardoso CP","year":"2013","journal-title":"Vet Parasitol"},{"issue":"2","key":"ref8","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1590\/S0100-736X2014000200008","article-title":"Resist\u00eancia contra ectoparasitas em bovinos da ra\u00e7a Crioula Lageana e meio-sangue Angus avaliada em condi\u00e7\u00f5es naturais","volume":"34","author":"Cardoso CP","year":"2014","journal-title":"Pesq Vet Bras"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"3190","DOI":"10.1128\/IAI.00008-06","article-title":"Involvement of gonadal steroids and gamma interferon in sex differences in response to Blood-Stage Malaria infection","volume":"74","author":"Cernetich A","year":"2006","journal-title":"Infect Immun"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ttbdis.2016.10.008","article-title":"Seroprevalence of Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginale antibodies in cattle in southern Egypt","volume":"8","author":"Fereig RM","year":"2017","journal-title":"Ticks Tick Borne Dis"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1998.tb11027.x","article-title":"Bovine Babesiosis and Anaplasmosis Follow\u2010up on Cattle relocated in an endemic area for hemoparasitic diseases","volume":"849","author":"Figueroa JV","year":"1998","journal-title":"Ann N Y Acad Sci"},{"issue":"1-2","key":"ref12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0304-4017(93)90008-B","article-title":"Multiplex polymerase chain reaction based assay for the detection of Babesia bigemina, Babesia bovis and Anaplasma marginale DNA in bovine blood","volume":"50","author":"Figueroa JV","year":"1993","journal-title":"Vet Parasitol"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"165","DOI":"10.5455\/jasa.20130411110618","article-title":"Ocorrence of antibodies against bovine Herpesvirus 1 and Bovine Viral Diarrhoea Virus in Crioula Lageana Cattle","volume":"3","author":"Fino TCM","year":"2013","journal-title":"J Anim Sci Adv"},{"issue":"9","key":"ref14","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1046\/j.1365-3024.2001.00402.x","article-title":"The age\u2010related immunity in cattle to Babesia bovis infection involves the rapid induction of interleukin\u201012, interferon\u2010\u03b3 and inducible nitric oxide synthase mRNA expression in the spleen","volume":"23","author":"Goff WL","year":"2001","journal-title":"Parasite Immunol"},{"issue":"1-3","key":"ref15","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0304-4017(94)03115-D","article-title":"Epidemiology of babesiosis and anaplasmosis in South and Central America","volume":"57","author":"Guglielmone AA","year":"1995","journal-title":"Vet Parasitol"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1590\/S1413-70542011000400024","article-title":"Soropreval\u00eancia e fatores de risco para Babesia bovis em rebanhos leiteiros na regi\u00e3o sul de Minas Gerais","volume":"35","author":"Guimar\u00e3es AM","year":"2011","journal-title":"Cienc Agrotec"},{"issue":"1-2","key":"ref17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2008.03.022","article-title":"Productivity and health effects of anaplasmosis and babesiosis on Bos indicus cattle and their crosses, and the effects of differing intensity of tick control in Australia","volume":"155","author":"Jonsson NN","year":"2008","journal-title":"Vet Parasitol"},{"issue":"8","key":"ref18","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/BF00930957","article-title":"Effects of testosterone on blood leukocytes in Plasmodium berghei-infected mice","volume":"75","author":"Kamis AB","year":"1989","journal-title":"Parasitol Res"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S0034-5288(03)00114-0","article-title":"Factors associated with bovine leptospirosis in Rio de Janeiro, Brazil","volume":"75","author":"Lilenbaum W","year":"2003","journal-title":"Res Vet Sci"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0065-308X(08)60285-7","article-title":"The piroplasms: life cycle and sexual stages","volume":"23","author":"Mehlhorn H","year":"1985","journal-title":"Adv Parasitol"},{"issue":"1","key":"ref21","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.ijpara.2004.09.002","article-title":"PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle","volume":"35","author":"Oliveira-Sequeira TCG","year":"2005","journal-title":"Int J Parasitol"},{"issue":"4","key":"ref22","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.ijpara.2009.09.009","article-title":"Tick-susceptible Bos taurus cattle display an increased cellular response at the site of larval Rhipicephalus (Boophilus) microplus attachment, compared with tick-resistant Bos indicus cattle","volume":"40","author":"Piper EK","year":"2010","journal-title":"Int J Parasitol"},{"issue":"10","key":"ref23","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1007\/s004360050639","article-title":"Influence of male gonadal hormones on the parasitemia and humoral response of male Calomys callosus infected with the Y strain of Trypanosoma cruzi.","volume":"85","author":"Prado Jr JC","year":"1999","journal-title":"Parasitol Res"},{"issue":"5","key":"ref24","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1071\/AR9640802","article-title":"The life cycle of Babesia bigemina (Smith and Kilborne, 1893) in the tick vector Boophilus microplus (Canestrini)","volume":"15","author":"Riek RF","year":"1964","journal-title":"Aust J Agric Res"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"367","DOI":"10.4269\/ajtmh.2012.12-0338","article-title":"Effect of sex steroids on Babesia microti infection in mice","volume":"88","author":"Sasaki M","year":"2013","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"ref26","first-page":"3139","article-title":"Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil","volume":"35","author":"Silva JB","year":"2014","journal-title":"Semina: Ci\u00eanc Agr\u00e1r"},{"issue":"2","key":"ref27","first-page":"95","article-title":"Epidemiologia de la babesiosis bovina. II. Indicadores epidemiol\u00f3gicos y elementos para el dise\u00f1o de estrategias de control","volume":"8","author":"Solorio-Rivera JL","year":"1997","journal-title":"Rev Biomed"},{"issue":"1","key":"ref28","first-page":"6","article-title":"Preval\u00eancia de anticorpos anti-Babesia em bovinos no Planalto Norte de Santa Catarina","volume":"1","author":"Souza AP","year":"2002","journal-title":"Rev Ci\u00eanc Agrovet"},{"key":"ref29","series-title":"Veterinary epidemiology.","author":"Thrusfield M","year":"2007","edition":"3rd"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/j.1751-0813.1978.tb02465.x","article-title":"The effect of age on resistance of cattle to Babesia bovis.","volume":"54","author":"Trueman KF","year":"1978","journal-title":"Aust Vet J"},{"issue":"1","key":"ref31","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1111\/j.1749-6632.1998.tb11087.x","article-title":"Seroprevalence of Anaplasma marginale in cattle in Parana State, Brazil, by MSP-5 competitive ELISA","volume":"849","author":"Vidotto MC","year":"2006","journal-title":"Ann N Y Acad Sci"},{"key":"ref32","doi-asserted-by":"crossref","DOI":"10.1016\/j.parepi.2019.e00114","article-title":"Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR","volume":"6","author":"Vieira LL","year":"2019","journal-title":"Parasite Epidemiol Control"},{"issue":"4","key":"ref33","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/j.1365-3024.2005.00748.x","article-title":"Possible mechanisms underlying age\u2010related resistance to bovine babesiosis","volume":"27","author":"Zintl A","year":"2005","journal-title":"Parasite Immunol"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":["Preval\u00eancia e fatores de risco associados com infec\u00e7\u00e3o por Babesia bigemina em bovinos da ra\u00e7a Crioula Lageana"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612025000100404&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T21:27:30Z","timestamp":1744061250000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612025000100404&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":33,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025]]}},"alternative-id":["S1984-29612025000100404"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612025018","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e005424"}}