{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T10:25:05Z","timestamp":1768645505886,"version":"3.49.0"},"reference-count":45,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for Research and Innovation Support of the State of Santa Catarina","award":["2021TR872"],"award-info":[{"award-number":["2021TR872"]}]},{"name":"Foundation for Research and Innovation Support of the State of Santa Catarina","award":["001"],"award-info":[{"award-number":["001"]}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["2021TR872"],"award-info":[{"award-number":["2021TR872"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Bovine trypanosomiasis, caused by infection with the protozoan Trypanosoma vivax, is harmful to livestock worldwide. Knowing its epidemiology is relevant to evaluate the susceptibility, resistance, and tolerance of animals. The objective of this study was to determine the prevalence of T. vivax in Crioula Lageana cattle and relate them to clinical, hematological, and biochemical findings to elucidate the breed\u2019s health and disease tolerance characteristics. Venous blood samples from 310 bovines considered healthy during the clinical examination were used to perform polymerase chain reaction (PCR) analysis, Immunofluorescence Antibody Test (IFAT) assays, hemogram tests, and serum biochemistry. The collected data were subjected to statistical analyses to compare seropositive and negative groups. IFAT indicated that the seroprevalence for T. vivax was 8% (24\/310); however, all tested animals were negative in the conventional PCR (0%, 0\/310). Higher amounts of platelets and less cholesterol were detected in seropositive animals but were within the reference values. Ruminal hypomotility and mild tachycardia were observed in all sampled animals. Considering the non-specific clinical signs and the absence of hematological alterations in infected animals, the seropositivity found indicates previous exposure to the protozoan, and the absence of clinically affected animals may result from characteristics inherent to this breed.<\/jats:p>","DOI":"10.1590\/s1984-29612025019","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T22:25:17Z","timestamp":1744842317000},"source":"Crossref","is-referenced-by-count":2,"title":["Molecular and serological survey of Trypanosoma vivax in Crioulo Lageano Cattle from southern Brazil"],"prefix":"10.1590","volume":"34","author":[{"given":"Felipe Eduardo","family":"Fiorin","sequence":"first","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana da Silva","family":"Casa","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Bergmann","family":"Griebeler","sequence":"additional","affiliation":[{"name":"Universidade Federal de Pelotas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Fuchs","family":"Goedel","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianlucca Sim\u00e3o Nadal","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Fl\u00e1vio Nepomuceno","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Universidade de Patos de Minas,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella Bassi das","family":"Neves","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio FACVEST,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graziela Vieira","family":"Fonteque","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio FACVEST,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Cl\u00e1udio","family":"Miletti","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil; Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mere Erika","family":"Saito","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil; Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1194-6048","authenticated-orcid":false,"given":"Joandes Henrique","family":"Fonteque","sequence":"additional","affiliation":[{"name":"Universidade do Estado de Santa Catarina,  Brasil; Universidade do Estado de Santa Catarina,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"1","key":"ref1","article-title":"Diagnostic, clinical and Epidemiological aspects of dairy cows naturally infected by Trypanosoma vivax in the states of Pernambuco and Alagoas, Brazil","volume":"41","author":"Andrade AQ","year":"2019","journal-title":"Rev Bras Med Vet"},{"issue":"4","key":"ref2","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1590\/1089-6891v17i434091","article-title":"Seroprevalence of Trypanosoma vivax, Anaplasma marginale, and Babesia bovis in dairy cattle","volume":"17","author":"Barbieri JM","year":"2016","journal-title":"Cienc Anim Bras"},{"issue":"12","key":"ref3","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1017\/S0031182020001006","article-title":"Epidemiological and molecular identification of Trypanosoma vivax diagnosed in cattle during outbreaks in central 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what is the role of adipose tissue in the life cycle of this protozoon?","volume":"30","author":"Machado RZ","year":"2021","journal-title":"Rev Bras Parasitol Vet"},{"key":"ref28","series-title":"Animais do descobrimento: ra\u00e7as dom\u00e9sticas da Hist\u00f3ria do Brasil","author":"Mariante AS","year":"2000"},{"key":"ref29","series-title":"Ra\u00e7a Crioula Lageana: O esteio de ontem, o labor de hoje e a oportunidade do amanh\u00e3.","author":"Martins VMV","year":"2009"},{"issue":"3","key":"ref30","first-page":"274","article-title":"Experimental bovine trypanosomiasis (Trypanosoma vivax and T. congolense). 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Sumptomatology and clinical pathology","volume":"30","author":"Maxie MG","year":"1979","journal-title":"Tropenmed Parasitol"},{"key":"ref31","article-title":"Trypanosoma vivax in and outside cattle blood: Parasitological, molecular, and serological detection, reservoir tissues, histopathological lesions, and vertical transmission evaluation","volume":"174","author":"Melo-Junior RD","year":"2024","journal-title":"Res Vet Sci"},{"key":"ref32","article-title":"First record of a possible trypanotolerant cattle breed in Latin America: Parasitological, serological, and clinical aspects","volume":"54","author":"Mendon\u00e7a DR","year":"2024","journal-title":"Vet Parasitol Reg Stud Rep"},{"issue":"6","key":"ref33","doi-asserted-by":"crossref","first-page":"761","DOI":"10.3136\/fstr.18.761","article-title":"Characterization of Commercial Cuts from the Crioulo Lageano Beef Breed","volume":"18","author":"Mitterer-Dalto\u00e9 ML","year":"2012","journal-title":"Food Sci Technol 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Infections","volume":"2013","author":"Sharma A","year":"2013","journal-title":"BioMed Res Int"},{"key":"ref40","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2022.105495","article-title":"Trypanosoma vivax infection in dairy cattle: parasitological and serological diagnosis and its relationship with the percentage of red blood cells","volume":"166","author":"Silva AS","year":"2022","journal-title":"Microb Pathog"},{"issue":"1","key":"ref41","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1111\/j.1749-6632.2000.tb05330.x","article-title":"Babesiosis (Babesia bovis) Stability in Unstable Environments","volume":"916","author":"Smith RD","year":"2000","journal-title":"Ann N Y Acad Sci"},{"issue":"267","key":"ref42","doi-asserted-by":"crossref","first-page":"338","DOI":"10.21071\/az.v69i267.5353","article-title":"Resistencia y resiliencia a las enfermedades en las razas de rumiantes locales: un enfoque en Am\u00e9rica del Sur","volume":"69","author":"Soares Fioravanti MC","year":"2020","journal-title":"Arch Zootec"},{"key":"ref43","series-title":"Veterinary epidemiology.","author":"Thrusfield M","year":"2007","edition":"3rd"},{"issue":"1","key":"ref44","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1006\/expr.2001.4641","article-title":"Trypanosoma vivax: characterization of the spliced-leader gene of a brazilian stock and species-specific detection by PCR amplification of an intergenic spacer sequence","volume":"99","author":"Ventura RM","year":"2001","journal-title":"Exp Parasitol"},{"issue":"4","key":"ref45","first-page":"384","article-title":"The haematocrit centrifuge technique for the diagnosis of African trypanosomiasis","volume":"27","author":"Woo PT","year":"1970","journal-title":"Acta Trop"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":["Pesquisa Molecular e Sorol\u00f3gica de Trypanosoma vivax em gado Crioulo Lageano"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612025000100406&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T22:25:25Z","timestamp":1744842325000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612025000100406&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":45,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025]]}},"alternative-id":["S1984-29612025000100406"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612025019","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e019424"}}