{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:16:40Z","timestamp":1780586200650,"version":"3.54.1"},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["302961\/2019-4"],"award-info":[{"award-number":["302961\/2019-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["303701\/2021-8"],"award-info":[{"award-number":["303701\/2021-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Equine Piroplasmosis (EP) is a tick-borne disease caused by the protozoan parasites Babesia caballi, Theileria equi, and Theileria haneyi, characterized by intravascular hemolysis and associated systemic illness. Although T. equi and B. caballi have been widely reported in some regions of Brazil, data from other states are limited. Additionally, despite reports of T. equi genotype C, currently recognized as T. haneyi, has been identified in Brazil, there are no investigations using T. haneyi-specific molecular tools. This study assessed the presence of these three agents in horses from Baixada Maranhense microregion (n = 34), northeastern Brazil, and in horses from an equestrian center (n = 12) in Guar\u00e1, southeastern Brazil. Of 46 horse DNA samples, one (2.1%) from an imported animal in the equestrian center tested positive for T. haneyi in ema-10 and ema-11-based PCR assays. Two animals tested positive for T. equi in a species-specific PCR (ema-1), and all samples were negative for B. caballi. BLASTn analysis showed ema-10 and ema-11 sequences shared 98.9% to 99.3% identity with T. haneyi detected in a horse at the U.S.-Mexico border. Despite the small sample size, this study confirms the presence of T. haneyi in Brazil and the need for monitoring imported animals.<\/jats:p>","DOI":"10.1590\/s1984-29612025049","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T15:02:49Z","timestamp":1760713369000},"source":"Crossref","is-referenced-by-count":2,"title":["Newly designed PCR assays based on the ema-10 and ema-11 genes confirm the circulation of Theileria haneyi in horses in Brazil"],"prefix":"10.1590","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5176-2940","authenticated-orcid":false,"given":"Rosangela Zacarias","family":"Machado","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Gomes","family":"Pereira","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria do Socorro Costa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa Sarmento dos Santos","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Maranh\u00e3o,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen Zilda Pereira de","family":"Toledo","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Ricardo","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Imunodot Diagn\u00f3sticos Veterin\u00e1rios,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/S0020-7519(01)00120-5","article-title":"Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks","volume":"31","author":"Battsetseg B","year":"2001","journal-title":"Int J Parasitol"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s11259-024-10618-3","article-title":"Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina","volume":"49","author":"Benitez-Ibalo AP","year":"2025","journal-title":"Vet Res Commun"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","DOI":"10.1016\/j.ttbdis.2019.101358","article-title":"Molecular genotyping and epidemiology of equine piroplasmids in South Africa","volume":"11","author":"Bhoora RV","year":"2020","journal-title":"Ticks Tick Borne Dis"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.vetpar.2008.10.004","article-title":"Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa","volume":"159","author":"Bhoora RV","year":"2009","journal-title":"Vet Parasitol"},{"issue":"3","key":"ref5","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1590\/s1984-29612017046","article-title":"Genetic diversity of piroplasmids species in equids from island of S\u00e3o Lu\u00eds, northeastern Brazil","volume":"26","author":"Braga MSCO","year":"2017","journal-title":"Rev Bras Parasitol Vet"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.ttbdis.2019.03.002","article-title":"Assessment of equine piroplasmids in the Nhecol\u00e2ndia sub-region of Brazilian Pantanal wetland using serological, parasitological, molecular, and hematological approaches","volume":"10","author":"Campos JBV","year":"2019","journal-title":"Ticks Tick Borne Dis"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1111\/evj.13579","article-title":"A risk assessment of equine piroplasmosis entry, exposureand consequences in the UK","volume":"55","author":"Coultous RM","year":"2023","journal-title":"Equine Vet J"},{"issue":"11","key":"ref8","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.3390\/pathogens10111414","article-title":"Rapid detection of equine piroplasms using multiplex PCR and first genetic characterization of Theileria haneyi in Egypt","volume":"10","author":"Elsawy BSM","year":"2021","journal-title":"Pathogens"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.cimid.2016.06.004","article-title":"Serological and molecular detection of Theileria equi in sport horses of northeastern Brazil","volume":"47","author":"Ferreira 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Brasil"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612025000300806&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T15:02:53Z","timestamp":1760713373000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612025000300806&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":24,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025]]}},"alternative-id":["S1984-29612025000300806"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612025049","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e005225"}}