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Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract The diversity of Hepatozoon spp., piroplasmids, and onchocercids that parasitize birds worldwide has been underestimated, especially in Brazil. The present work aimed to investigate, using molecular assays, the occurrence of Hepatozoon spp., piroplasmids, and onchocercids in tropical birds from the Brazilian Pantanal wetland, in the states of Mato Grosso and Mato Grosso do Sul. Blood sampling and DNA extraction were performed on 517 birds from 13 avian orders. DNA samples positive to endogenous gene (avian \u03b2-actin) were subjected to PCR assays targeting the 18S rRNA gene of Hepatozoon spp. and piroplasmids as well as PCR assays for onchocercids (cox-1, 28S rRNA and 18S rRNA genes). As a result, two onchocercids (0.4%) were identified. None was positive in the PCR assays for Hepatozoon spp. or piroplasmids. The cox-1 sequence detected in Ramphocelus carbo grouped with Cardiofilaria sp., and onchocercid cox-1 sequence obtained from Taraba major grouped with Splendidofilaria spp. This is the first molecular report of onchocercids closely related to Cardiofilaria spp. and Splendidofilaria spp. in birds from the Brazilian Pantanal.<\/jats:p>","DOI":"10.1590\/s1984-29612025065","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T14:57:39Z","timestamp":1763477859000},"source":"Crossref","is-referenced-by-count":0,"title":["Molecular survey of Hepatozoon spp., piroplasmids, and onchocercids in wild birds from the Brazilian Pantanal"],"prefix":"10.1590","volume":"34","author":[{"given":"Amir Salvador","family":"Alab\u00ed C\u00f3rdova","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Cl\u00e1udia","family":"Calchi","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Fecchio","sequence":"additional","affiliation":[{"name":"Academy of Natural Sciences of Drexel University,  USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizeth Fernanda","family":"Banguero-Micolta","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1007\/s11259-024-10341-z","article-title":"Molecular evidence of Bartonella spp. in tropical wild birds from the Brazilian Pantanal, the largest wetland in South America","volume":"48","author":"Alab\u00ed C\u00f3rdova AS","year":"2024","journal-title":"Vet Res Commun"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"962","DOI":"10.3390\/microorganisms12050962","article-title":"Novel tick-borne Anaplasmataceae genotypes in tropical birds from the Brazilian Pantanal wetland","volume":"12","author":"Alab\u00ed C\u00f3rdova AS","year":"2024","journal-title":"Microorganisms"},{"issue":"5","key":"ref3","doi-asserted-by":"crossref","first-page":"491","DOI":"10.3390\/pathogens14050491","article-title":"Validation of a high-throughput microfluidic real-time 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(Nematoda: Spirurida: Filarioidea) in Mexican ramphastids","volume":"11","author":"Sanchez-Godoy FD","year":"2020","journal-title":"Int J Parasitol Parasites Wildl"},{"issue":"20","key":"ref27","doi-asserted-by":"crossref","first-page":"R1149","DOI":"10.1016\/j.cub.2022.08.061","article-title":"Passerine birds","volume":"32","author":"Schmitt CJ","year":"2022","journal-title":"Curr Biol"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1590\/S1984-29612012000100003","article-title":"Blood parasites in passerine birds from the Brazilian Atlantic Forest","volume":"21","author":"Sebaio F","year":"2012","journal-title":"Rev Bras Parasitol Vet"},{"issue":"8","key":"ref29","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1007\/s00436-023-07894-4","article-title":"A novel variant of Babesia sp. (Piroplasmida) as a hemoparasite in procellariiform seabirds","volume":"122","author":"Sgarioni AZ","year":"2023","journal-title":"Parasitol Res"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1590\/S1984-29612014031","article-title":"New record of Pelecitus sp. (Nematoda, Onchocercidae) as a parasite of Athene cunicularia (Strigiformes, Strigidae) in southeastern Brazil","volume":"23","author":"Silva TM","year":"2014","journal-title":"Rev Bras Parasitol Vet"},{"issue":"4","key":"ref31","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.7589\/0090-3558-46.4.1305","article-title":"Microfilariae infection in wild birds from the Brazilian Cerrado","volume":"46","author":"Silveira P","year":"2010","journal-title":"J Wildl Dis"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s13071-024-06154-3","article-title":"Hepatozoon (Eucoccidiorida: Hepatozoidae) in wild mammals of the Americas: a systematic review","volume":"17","author":"Thomas R","year":"2024","journal-title":"Parasit Vectors"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.cimid.2017.09.003","article-title":"Arthropod-borne agents in wild Orinoco geese (Neochen jubata) in Brazil","volume":"55","author":"Werther K","year":"2017","journal-title":"Comp Immunol Microbiol Infect Dis"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":["Investiga\u00e7\u00e3o molecular de Hepatozoon spp., piroplasm\u00eddeos e oncocerc\u00eddeos em aves selvagens do Pantanal brasileiro"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612025000400405&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T14:57:51Z","timestamp":1763477871000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612025000400405&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":33,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025]]}},"alternative-id":["S1984-29612025000400405"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612025065","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e010725"}}