{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:05:18Z","timestamp":1764601518828,"version":"3.46.0"},"reference-count":40,"publisher":"FapUNIFESP (SciELO)","issue":"4","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["2020\/001"],"award-info":[{"award-number":["2020\/001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Fasciolosis is a foodborne anthropozoonotic disease caused by Fasciola spp. that affects multiple hosts worldwide. Genetic characterization studies have revealed considerable diversity within F. hepatica populations owing to human and animal migration and intermediate snail hosts. Molecular markers such as microsatellites and mitochondrial DNA sequences are useful tools for assessing parasite population dynamics and evolutionary history. In this study, 16 F. hepatica samples were analyzed using cytochrome c oxidase 1 (CO1) and nicotinamide dehydrogenase (NADH) genes. Parasites were collected from the livers of naturally infected cattle originating from 14 municipalities in Paran\u00e1, Brazil. Both CO1 and NADH sequences showed high homogeneity, with mean genetic distances &lt;1% (0.0084 and 0.0092, respectively). Network analyses revealed seven CO1 haplotypes and four NADH haplotypes among the new Paran\u00e1 samples. When compared with reference sequences available in GenBank from other Brazilian states (Rio Grande do Sul and S\u00e3o Paulo), the Paran\u00e1 samples also displayed &lt;1% genetic divergence. This high level of homogeneity suggests a common and stable origin, with limited gene flow across regions. The genetic information reported here can support the development of targeted interventions, such as vaccines and drugs, aimed at controlling similar F. hepatica genetic variants.<\/jats:p>","DOI":"10.1590\/s1984-29612025069","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:03:14Z","timestamp":1764601394000},"source":"Crossref","is-referenced-by-count":0,"title":["Genetic characterization of Fasciola hepatica (Linnaeus, 1758) in cattle from Paran\u00e1, Brazil"],"prefix":"10.1590","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3790-6335","authenticated-orcid":false,"given":"Desiree Vera","family":"Pontarolo","sequence":"first","affiliation":[{"name":"Universidade Federal do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto Augusto","family":"Krug","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Mesquita","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Angelo Sganzerla","family":"Graichen","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Beltr\u00e3o","family":"Molento","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1,  Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.4081\/gh.2014.22","article-title":"Impact of climate change and man-made irrigation systems on the transmission risk, long-term trend and seasonality of human and animal fascioliasis in 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region","volume":"102","author":"Valero MA","year":"2001","journal-title":"Vet Parasitol"},{"issue":"3","key":"ref38","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1017\/S1751731109990991","article-title":"Climate change and infectious disease: helminthological challenges to farmed ruminants in temperate regions","volume":"4","author":"van Dijk J","year":"2010","journal-title":"Animal"},{"issue":"6","key":"ref39","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1017\/S0022149X15001029","article-title":"Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba","volume":"90","author":"V\u00e1zquez AA","year":"2016","journal-title":"J Helminthol"},{"issue":"2","key":"ref40","doi-asserted-by":"crossref","first-page":"199","DOI":"10.2478\/jvetres-2022-0018","article-title":"Molecular characteristics and genetic diversity of Fasciola hepatica from Sheep in Xinjiang, China","volume":"66","author":"Xifeng 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Brasil"],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612025000400407&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:03:20Z","timestamp":1764601400000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612025000400407&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":40,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025]]}},"alternative-id":["S1984-29612025000400407"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612025069","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e008325"}}