{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:07:42Z","timestamp":1793660862871,"version":"4.5.1"},"reference-count":25,"publisher":"FapUNIFESP (SciELO)","issue":"2","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Coinfection with Trypanosoma evansi and Hepatozoon felis represents a rare occurrence in wild felids, with no previous reports in Panthera onca. A free-ranging adult male jaguar weighing approximately 70 kg was examined after being rescued from wildfires in the Brazilian Pantanal (Corumb\u00e1, Mato Grosso do Sul). The initial hematological evaluation (D0) revealed mild anemia (PCV: 27.7%; reference: 30\u201345%), with Trypanosoma spp. trypomastigotes forms detected by direct microscopy. Treatment with diminazene aceturate (3.5 mg\/kg, IM) was instituted on D1. On D5, microscopy revealed Hepatozoon felis gamonts within leukocytes, characterizing coinfection. Molecular analysis by PCR confirmed the presence of both agents in the D0 sample, with amplification products of approximately 600 bp (Hepatozoon spp.) and 196 bp (T. evansi), validating the microscopic diagnosis. Despite treatment and the absence of microscopically detectable parasitemia on D12, anemia persisted (PCV: 21.7%). The animal progressed to death on D19 during sedation for wound management. At necropsy, severe pulmonary involvement (bronchiolitis, alveolitis, and endogenous lipid pneumonia) compatible with smoke inhalation injury was observed. These findings highlight the importance of integrating hemoparasite surveillance into rehabilitation and conservation protocols for threatened felids in fire-affected ecosystems.<\/jats:p>","DOI":"10.1590\/s1984-29612026033","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T11:28:46Z","timestamp":1784546926000},"source":"Crossref","is-referenced-by-count":1,"title":["Coinfection with Trypanosoma evansi and Hepatozoon felis in a free-ranging jaguar (Panthera onca) in the Pantanal biome, Brazil"],"prefix":"10.1590","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5784-0727","authenticated-orcid":false,"given":"Brenda Maria Emanuela Silva","family":"Reis","sequence":"first","affiliation":[{"name":"Universidade Federal de Mato Grosso do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vithoria Eduarda","family":"Barboza Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordana","family":"Toqueto","sequence":"additional","affiliation":[{"name":"Instituto de Meio Ambiente de Mato Grosso do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma Gabrieli","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto de Meio Ambiente de Mato Grosso do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie Carrelo de","family":"Lima","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alda Izabel","family":"de Souza","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Alberto do Nascimento","family":"Ramos","sequence":"additional","affiliation":[{"name":"Universidade Federal de Mato Grosso do Sul,  Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"key":"ref1","article-title":"Molecular detection of Hepatozoon spp. and Cytauxzoon felis in wild carnivorans and domestic dogs in southern Pantanal wetlands of Brazil with new host records","volume":"59","author":"Alves MH","year":"2025","journal-title":"Vet Parasitol Reg Stud Reports"},{"issue":"1-2","key":"ref2","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.vetpar.2010.06.014","article-title":"Molecular detection of Hepatozoon spp. in Brazilian and exotic wild carnivores","volume":"173","author":"Andr\u00e9 MR","year":"2010","journal-title":"Vet Parasitol"},{"key":"ref3","series-title":"Avalia\u00e7\u00e3o de um protocolo de extra\u00e7\u00e3o de DNA gen\u00f4mico a partir de sangue total.","author":"Ara\u00fajo 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