{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:03:17Z","timestamp":1791198197405,"version":"4.1.0"},"reference-count":38,"publisher":"FapUNIFESP (SciELO)","issue":"3","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"INTA","award":["I115 P05 A025"],"award-info":[{"award-number":["I115 P05 A025"]}]},{"name":"INTA","award":["I113 P22 A03"],"award-info":[{"award-number":["I113 P22 A03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract Heterologous antibody\u2011mediated immunity was evaluated in cattle naturally infected with Ostertagia ostertagi (Oo) and Haemonchus sp. (Hp). Antibody responses were assessed using ovalbumin (OVA) as unrelated model antigen in three groups of eight heifers, matched for eggs per gram of feces (EPG) and allocated to independent, nematode\u2011free pastures. Anti\u2011OVA titers were determined in serum by ELISA. EPG counts and nematode genera were determined in feces using the modified McMaster technique and pooled cultures, respectively. All variables were analyzed using mixed models. One group was previously dewormed and considered the control (C) group. A second group was designated HO (90.4% Hp and 7.8% Oo). A third group was designated OH (19.6% Oo and 78.8% Hp). EPGs were absent in the C group, and no EPG differences were observed between the parasitized groups throughout the study. The OH group exhibited a lower anti\u2011OVA titer peak compared with the C (p = 0.015) and HO (p = 0.024) groups. Moderate prevalences of Oo may reduce heterologous antibody peaks without affecting the overall curve, whereas high prevalences of Hp may not alter heterologous antibody-based responses in cattle.<\/jats:p>","DOI":"10.1590\/s1984-29612026046","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:38:17Z","timestamp":1791196697000},"source":"Crossref","is-referenced-by-count":0,"title":["Heterologous antibody-based immunity in cattle naturally infected with Ostertagia ostertagi and Haemonchus sp."],"prefix":"10.1590","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6797-0509","authenticated-orcid":false,"given":"Dami\u00e1n Jes\u00fas","family":"Castro","sequence":"first","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","place":["C\u00f3rdoba, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego Daniel","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Instituto de Virolog\u00eda e Innovaciones Tecnol\u00f3gicas","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Augusto","family":"Margineda","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","place":["C\u00f3rdoba, Argentina"]},{"name":"Universidad Nacional de Rosario","place":["Santa Fe, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"530","published-online":{"date-parts":[[2026]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"2264","DOI":"10.3390\/ijms26052264","article-title":"Transcriptional profiling of abomasal mucosa from young calves experimentally infected with Ostertagia ostertagi.","volume":"26","author":"Boschiero C","year":"2025","journal-title":"Int J Mol Sci"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/j.rvsc.2013.09.001","article-title":"Serological evidence of Ostertagia ostertagi infection in dairy cows does not impact the efficacy of rabies vaccination during the housing period","volume":"95","author":"Charlier J","year":"2013","journal-title":"Res Vet Sci"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cvfa.2019.11.001","article-title":"Biology and epidemiology of gastrointestinal nematodes in cattle","volume":"36","author":"Charlier J","year":"2020","journal-title":"Vet Clin North Am Food Anim Pract"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.pt.2022.11.014","article-title":"To treat or not to treat: diagnostic thresholds in subclinical helminth infections of cattle","volume":"39","author":"Charlier J","year":"2023","journal-title":"Trends Parasitol"},{"issue":"1","key":"ref5","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.cvfa.2017.10.008","article-title":"Gastrointestinal nematodes, diagnosis and control","volume":"34","author":"Craig TM","year":"2018","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"ref6","first-page":"25","article-title":"Anthelmintic resistance in grazing beef cattle in central and northeastern areas of Argentina: an update","volume":"9","author":"Cristel S","year":"2017","journal-title":"Vet Parasitol Reg Stud Reports"},{"issue":"1&#8211;2","key":"ref7","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0304-4017(86)90006-3","article-title":"Lymphocyte blastogenic responses of calves experimentally infected with Ostertagia ostertagi.","volume":"22","author":"Cross DA","year":"1986","journal-title":"Vet Parasitol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.exppara.2016.09.017","article-title":"Experimental infection of calves with Haemonchus placei or Haemonchus contortus: assessment of clinical, hematological and biochemical parameters and histopathological characteristics of abomasums","volume":"170","author":"F\u00e1vero FC","year":"2016","journal-title":"Exp Parasitol"},{"key":"ref9","article-title":"Helminth infections affect host immune responses to viral infections and vaccines","volume":"49","author":"Garc\u00eda-Bernalt Diego J","year":"2025","journal-title":"FEMS Microbiol Rev"},{"issue":"3&#8211;4","key":"ref10","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0304-4017(97)00104-0","article-title":"Effect of gastrointestinal nematode infection on the ruminant immune system","volume":"72","author":"Gasbarre LC","year":"1997","journal-title":"Vet Parasitol"},{"issue":"2&#8211;3","key":"ref11","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/0304-4017(91)90126-G","article-title":"Pathophysiology of Haemonchus placei infection in calves","volume":"38","author":"Gennari SM","year":"1991","journal-title":"Vet Parasitol"},{"issue":"4","key":"ref12","first-page":"620","article-title":"The potential immunomodulatory effect of levamisole in humans and farm animals","volume":"10","author":"Gholami MH","year":"2024","journal-title":"J Adv Vet Anim Res"},{"issue":"3","key":"ref13","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1046\/j.1365-2567.2000.00974.x","article-title":"Induction of T helper 1- and T helper 2-type immune responses during Haemonchus contortus infection in sheep","volume":"99","author":"Gill HS","year":"2000","journal-title":"Immunology"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.vetpar.2004.01.006","article-title":"Inhibition of bovine T lymphocyte responses by extracts of the stomach worm Ostertagia ostertagi.","volume":"120","author":"G\u00f3mez-Mu\u00f1oz MT","year":"2004","journal-title":"Vet Parasitol"},{"issue":"3-4","key":"ref15","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0168-1591(94)90026-4","article-title":"Relationship between immune response, liveweight gain, behaviour and adrenal function in red deer (Cervus elaphus) calves derived from wild and farmed stock, maintained at two housing densities","volume":"41","author":"Hanlon AJ","year":"1994","journal-title":"Appl Anim Behav Sci"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0021-9975(71)90064-8","article-title":"Experimental Haemonchus placei infection in calves. Influence of anaemia and numbers of larvae on worm development","volume":"81","author":"Harness E","year":"1971","journal-title":"J Comp Pathol"},{"key":"ref17","series-title":"Clinical biochemistry of domestic animals.","author":"Kaneko JJ","year":"2008","edition":"6th"},{"issue":"2","key":"ref18","first-page":"106","article-title":"The measurement of total serum proteins by the biuret method","volume":"8","author":"Lubran MM","year":"1978","journal-title":"Ann Clin Lab Sci"},{"issue":"3-4","key":"ref19","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0304-4017(91)90049-2","article-title":"Immune modulation by Ostertagia ostertagi and the effects of diet","volume":"39","author":"Mansour MM","year":"1991","journal-title":"Vet Parasitol"},{"issue":"3","key":"ref20","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0165-2427(92)90186-T","article-title":"Modulation of calf immune responses by Ostertagia ostertagi: the effect of diet during trickle infection","volume":"33","author":"Mansour MM","year":"1992","journal-title":"Vet Immunol Immunopathol"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1017\/S0031182000031127","article-title":"Some observations on the worm burdens of calves infected daily with Ostertagia ostertagi.","volume":"59","author":"Michel JF","year":"1969","journal-title":"Parasitology"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1177\/104063878900100225","article-title":"Ostertagiasis in cattle","volume":"1","author":"Myers GH","year":"1989","journal-title":"J Vet Diagn Invest"},{"key":"ref23","series-title":"Enfermedades parasitarias de importancia cl\u00ednica y productiva en rumiantes: fundamentos epidemiol\u00f3gicos para su diagn\u00f3stico y control.","author":"Nari A","year":"2013","edition":"2nd"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1071\/AR9500099","article-title":"Methods for egg counts and larval cultures for strongyles infesting the gastro-intestinal tract of cattle","volume":"1","author":"Roberts FHS","year":"1950","journal-title":"Aust J Agric Res"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s00436-024-08228-8","article-title":"Biomarkers of gastrointestinal nematodes in beef cattle raised in a tropical area","volume":"123","author":"Rodrigues VD","year":"2024","journal-title":"Parasitol Res"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s13071-023-05680-w","article-title":"Practical guide to the diagnostics of ruminant gastrointestinal nematodes, liver fluke and lungworm infection: interpretation and usability of results","volume":"16","author":"Sabatini GA","year":"2023","journal-title":"Parasit Vectors"},{"issue":"Pt 3","key":"ref27","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1017\/S0031182005009108","article-title":"Immunomodulatory effect of various anti-parasitics: a review","volume":"132","author":"Sajid MS","year":"2006","journal-title":"Parasitology"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0165-2427(86)90005-X","article-title":"Immunosupression of lymphocite blastogenesis in cattle infected with Ostertagia ostertagi and\/or Trichostrongylus axei.","volume":"11","author":"Snider 3rd TG","year":"1986","journal-title":"Vet Immunol Immunopathol"},{"issue":"3-4","key":"ref29","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0304-4017(89)90076-9","article-title":"A Comparison of two methods and two additives for faecal cultivation of bovine trichostrongyle larvae","volume":"31","author":"Steffan P","year":"1989","journal-title":"Vet Parasitol"},{"key":"ref30","series-title":"Enfermedades parasitarias de importancia cl\u00ednica y productiva en rumiantes: fundamentos epidemiol\u00f3gicos para su diagn\u00f3stico y control.","first-page":"59","volume-title":"Epidemiolog\u00eda e impacto productivo de nematodos en la Pampa Central de Argentina.","author":"Su\u00e1rez V","year":"2013","edition":"2nd"},{"issue":"6","key":"ref31","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.imbio.2007.03.009","article-title":"Chronic helminth infections induce immunomodulation: consequences and mechanisms","volume":"212","author":"van Riet E","year":"2007","journal-title":"Immunobiology"},{"issue":"35","key":"ref32","doi-asserted-by":"crossref","first-page":"5582","DOI":"10.1016\/j.vaccine.2020.06.064","article-title":"Do parasite infections interfere with immunisation? A review and meta-analysis","volume":"38","author":"Wait LF","year":"2020","journal-title":"Vaccine"},{"issue":"1","key":"ref33","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/0009-8981(74)90357-X","article-title":"An assessment of the suitability of bromocresol green for the determination of serum albumin","volume":"53","author":"Webster D","year":"1974","journal-title":"Clin Chim Acta"},{"issue":"10","key":"ref34","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.2460\/ajvr.1989.50.10.1764","article-title":"Studies of the immunomodulatory effects of low-level infection with Ostertagia ostertagi in calves","volume":"50","author":"Wiggin CJ","year":"1989","journal-title":"Am J Vet Res"},{"issue":"7","key":"ref35","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.2460\/ajvr.1993.54.07.1074","article-title":"Immunologic changes in Ostertagia ostertagi-infected calves treated strategically with an anthelmintic","volume":"54","author":"Yang C","year":"1993","journal-title":"Am J Vet Res"},{"issue":"12","key":"ref36","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.2460\/ajvr.1993.54.12.2048","article-title":"Prevention of pathophysiologic and immunomodulatory effects of gastrointestinal nematodiasis in calves by use of strategic anthelmintic treatments","volume":"54","author":"Yang C","year":"1993","journal-title":"Am J Vet Res"},{"key":"ref37","article-title":"The combination of vaccines and adjuvants to prevent the occurrence of high incidence of infectious diseases in bovine","volume":"10","author":"Yao Y","year":"2023","journal-title":"Front Vet Sci"},{"issue":"3","key":"ref38","doi-asserted-by":"crossref","first-page":"179","DOI":"10.2174\/1573395509666131126230832","article-title":"Dysfunctional adaptive immunity during parasitic infections","volume":"9","author":"Zander RA","year":"2013","journal-title":"Curr Immunol Rev"}],"container-title":["Revista Brasileira de Parasitologia Veterin\u00e1ria"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S1984-29612026000300209&tlng=en","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:38:23Z","timestamp":1791196703000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1984-29612026000300209&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":38,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026]]}},"alternative-id":["S1984-29612026000300209"],"URL":"https:\/\/doi.org\/10.1590\/s1984-29612026046","relation":{},"ISSN":["1984-2961","0103-846X"],"issn-type":[{"value":"1984-2961","type":"electronic"},{"value":"0103-846X","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e010726"}}