{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T23:04:24Z","timestamp":1768691064096,"version":"3.49.0"},"reference-count":24,"publisher":"FapUNIFESP (SciELO)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. Dent. J."],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p>Abstract The aim of this study was to evaluate the density and the morphological aspects of biofilms adhered to different materials applied in oral rehabilitation supported by dental implants. Sixty samples were divided into four groups: feldspar-based porcelain, CoCr alloy, commercially pure titanium grade IV and yttria-stabilized zirconia. Human saliva was diluted into BHI supplemented with sucrose to grow biofilms for 24 or 48 h. After this period, biofilm was removed by 1% protease treatment and then analyzed by spectrophotometry (absorbance), colony forming unit method (CFU.cm-2) and field-emission guns scanning electron microscopy (FEG-SEM). The highest values of absorbance and CFU.cm-2 were recorded on biofilms grown on CoCr alloys when compared to the other test materials for 24 or 48 h. Also, FEG-SEM images showed a high biofilm density on CoCr. There were no significant differences in absorbance and CFU.cm-2 between biofilms grown on zirconia, porcelain and titanium (p&lt;0.05). Microbiological assays associated with microscopic analyses detected a higher accumulation of oral biofilms on CoCr-based materials than that on titanium or zirconia that are used for prosthetic structures.<\/jats:p>","DOI":"10.1590\/0103-6440201600625","type":"journal-article","created":{"date-parts":[[2016,4,2]],"date-time":"2016-04-02T21:50:02Z","timestamp":1459633802000},"page":"141-147","source":"Crossref","is-referenced-by-count":47,"title":["Biofilm Formation on Different Materials Used in Oral Rehabilitation"],"prefix":"10.1590","volume":"27","author":[{"given":"J\u00falio C. M.","family":"Souza","sequence":"first","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil; Universidade do Minho,  Portugal"}]},{"given":"Raquel R. C.","family":"Mota","sequence":"additional","affiliation":[{"name":"Universidade Fernando Pessoa,  Portugal"}]},{"given":"Mariane B.","family":"Sordi","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil"}]},{"given":"Bernardo B.","family":"Passoni","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil"}]},{"given":"Cesar A. M.","family":"Benfatti","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil"}]},{"given":"Ricardo S.","family":"Magini","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Catarina,  Brazil"}]}],"member":"530","reference":[{"key":"ref1","series-title":"Oral Microbiology","author":"Marsh PD","year":"1999","edition":"4th ed."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1600-0501.2006.01353.x","article-title":"Effect of material characteristics and\/or surface topography on biofilm development","volume":"17","author":"Teughels W","year":"2006","journal-title":"Clin Oral Implants Res"},{"key":"ref3","series-title":"Clinical periodontology and implant dentistry","author":"Karring T","year":"2008","edition":"5th ed."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/978-3-319-11038-7_4","article-title":"Peri-implant infections of oral biofilm etiology","volume":"830","author":"Belibasakis GN","year":"2015","journal-title":"Adv Exp Med Biol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1128\/MMBR.66.3.486-505.2002","article-title":"Communications among oral bacteria","volume":"66","author":"Kolenbrander PE","year":"2002","journal-title":"Microbiol Mol Bio Rev"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3201\/eid0809.020063","article-title":"Biofilms: microbial life on surfaces","volume":"8","author":"Donlan RM.","year":"2002","journal-title":"Emerg Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1111\/j.1600-051X.1990.tb01077.x","article-title":"The influence of surface free energy and surface roughness on early plaque formation. 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