{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:06:29Z","timestamp":1753887989221,"version":"3.41.2"},"reference-count":29,"publisher":"FapUNIFESP (SciELO)","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"FCT","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"FCT","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]},{"name":"WP9","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"WP9","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"WP9","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]},{"name":"PRR","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"PRR","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"PRR","award":["C644915664-00000026"],"award-info":[{"award-number":["C644915664-00000026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Bot. Bras."],"abstract":"<jats:p>Abstract This study aims to reevaluate the diversity of 16S rRNA gene sequences associated with Scytonema (Nostocales, Cyanobacteria). For that, we first conducted a search for \u2018Scytonema\u2019 sequences available in GenBank (NCBI) and aligned the results with reference strains from other Nostocales genera. The alignment totalized 383 OTUs and 800 nucleotide positions analyzed. A FastTree phylogenetic analysis revealed that the \u2018Scytonema\u2019 sequences are distributed across 15 distinct clades in addition to the Scytonema sensu stricto clade. These additional clades are positioned among Nostocales genera and phylogenetically distant from the type species, S. hofmanni. Within these clades, we had access to the strain Scytonema sp. CCIBt3568, identified as S. schmidtii, which we believe should be separated from Scytonema, and described as a new genus in the future. Additionally, Bayesian Inference and Maximum Likelihood phylogenies were performed with 97 OTUs and 971 bp, and the polyphyletic status of Scytonema as currently recognized was confirmed.<\/jats:p>","DOI":"10.1590\/1677-941x-abb-2024-0044","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T11:12:00Z","timestamp":1748603520000},"source":"Crossref","is-referenced-by-count":0,"title":["Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont"],"prefix":"10.1590","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4396-024X","authenticated-orcid":false,"given":"Guilherme Scotta","family":"Hentschke","sequence":"first","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research,  Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7706-756X","authenticated-orcid":false,"given":"C\u00e9lia Leite","family":"Sant\u2019Anna","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisas Ambientais,  Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"Vitor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research,  Portugal; University of Porto,  Portugal"}]}],"member":"530","published-online":{"date-parts":[[2025]]},"reference":[{"key":"ref1","first-page":"51","article-title":"Revision des Nostocac\u00e9es h\u00e9t\u00e9rocyst\u00e9es contenues dans les principaux herbiers de France (Troisi\u00e8me fragment)","volume":"5","author":"Bornet \u00c9","year":"1887","journal-title":"Annales des Sciences Naturelles, Botanique"},{"year":"1959","series-title":"Cyanophyta","author":"Desikachary TV","key":"ref2"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1111\/j.1529-8817.2007.00376.x","article-title":"The cyanobacterial genus Brasilonema, gen. nov., a molecular and phenotypic evaluation","volume":"43","author":"Fiore MF","year":"2007","journal-title":"Journal of Phycology"},{"year":"1927","series-title":"New Myxophyceae from Porto Rico","author":"Gardner NL","key":"ref4"},{"key":"ref5","series-title":"Kryptogamen Flora von Deutschl and, \u00d6sterreichund Der Schweiz","volume-title":"Cyanophyceae","author":"Geitler L","year":"1932"},{"key":"ref6","series-title":"The Pharmacological Potential of Cyanobacteria","first-page":"1","volume-title":"Trends in Cyanobacteria: A contribution to systematics and biodiversity studies","author":"Hentschke GS","year":"2022"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s40415-014-0069-y","article-title":"Scytonema santannae, a new morphospecies of Cyanobacteria from the Atlantic rainforest, southeastern Brazil","volume":"37","author":"Hentschke GS","year":"2014","journal-title":"Brazilian Journal of Botany"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1127\/algol_stud\/2014\/0185","article-title":"Current trends and prospects for cyanobacterial taxonomy - are only cultured populations enough?","volume":"147","author":"Hentschke GS","year":"2015","journal-title":"Algological Studies"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"63","DOI":"10.5252\/cryptogamie-algologie2024v45a6","article-title":"A journey through cyanobacteria in Brazil: A comprehensive review of genera and 16S rDNA sequence analyses","volume":"45","author":"Hentschke G","year":"2024","journal-title":"Cryptogamie Algologie"},{"key":"ref10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0186393","article-title":"Highly divergent 16S rRNA sequences in ribosomal operons of Scytonema hyalinum (Cyanobacteria)","volume":"12","author":"Johansen JR","year":"2017","journal-title":"PLoS One"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1007\/s10750-023-05356-7","article-title":"Welcome to the jungle!: An overview of modern taxonomy of cyanobacteria","volume":"851","author":"Kastovsky J","year":"2023","journal-title":"Hydrobiologia"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh K","year":"2002","journal-title":"Nucleic Acids Research"},{"key":"ref13","series-title":"S\u00fc\u00dfwasserflora von Mitteleuropa 19\/3, XVIII","volume-title":"Cyanoprokaryota: 3rd Part: Heterocystous Genera","author":"Kom\u00e1rek J","year":"2013"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"173","DOI":"10.5507\/fot.2013.015","article-title":"Phenotype diversity and phylogeny of selected Scytonema-species (Cyanoprokaryota) from SE Brazil","volume":"13","author":"Kom\u00e1rek J","year":"2013","journal-title":"Fottea"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1111\/jpy.13335","article-title":"CyanoSeq: A database of cyanobacterial 16S rRNA sequences with curated taxonomy","volume":"59","author":"Lefler DE","year":"2023","journal-title":"Journal of Phycology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (iTOL) v5: An online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic I","year":"2021","journal-title":"Nucleic Acids Research"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1111\/jpy.13396","article-title":"Description of four new filamentous cyanobacterial taxa from freshwater habitats in the Azores Archipelago","volume":"59","author":"Luz R","year":"2023","journal-title":"Journal of Phycology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.hal.2016.11.021","article-title":"Iningainema pulvinus gen nov., sp nov. 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