{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T06:10:37Z","timestamp":1708236637320},"reference-count":23,"publisher":"FapUNIFESP (SciELO)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Soc. Bras. Med. Trop."],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p>Foram analisadas a freq\u00fc\u00eancia e distribui\u00e7\u00e3o de muta\u00e7\u00f5es nos genes dihidrofolato redutase e dihidropteroato sintetase do Plasmodium falciparum, usando a metodologia de rea\u00e7\u00e3o em cadeia da polimerase e polimorfismos de hidr\u00f3lise por enzimas de restri\u00e7\u00e3o, em amostras de sangue infectado proveniente de crian\u00e7as mo\u00e7ambicanas, residentes em Maputo. A an\u00e1lise foi feita antes e 7 dias ap\u00f3s o tratamento com sulfadoxina-pirimetamina (S\/P). Os resultados mostraram a ocorr\u00eancia de muta\u00e7\u00f5es pontuais nos genes estudados e a presen\u00e7a de combina\u00e7\u00f5es de tr\u00eas alelos em dhfr (51Ile, 59Arg e 108Asn) e do quint\u00faplo mutante (dhfr 51Ile, 59Arg, 108Asn e dhps 437Gly, 540Glu), ambas situa\u00e7\u00f5es associadas \u00e0 falha terap\u00eautica no s\u00e9timo dia ap\u00f3s tratamento com S\/P. Esses achados mostram a import\u00e2ncia de se estudar a resist\u00eancia \u00e0 S\/P em Mo\u00e7ambique, e como os marcadores moleculares de resist\u00eancia aos antimal\u00e1ricos podem fornecer dados importantes para a pol\u00edtica nacional de controlo da mal\u00e1ria.<\/jats:p>","DOI":"10.1590\/s0037-86822007000400015","type":"journal-article","created":{"date-parts":[[2007,9,27]],"date-time":"2007-09-27T16:17:47Z","timestamp":1190909867000},"page":"447-450","source":"Crossref","is-referenced-by-count":1,"title":["Resist\u00eancia \u00e0 sulfadoxina-pirimetamina em Maputo, Mo\u00e7ambique: presen\u00e7a de muta\u00e7\u00f5es nos genes dhfr e dhps do Plasmodium falciparum"],"prefix":"10.1590","volume":"40","author":[{"given":"Nat\u00e9rcia Em\u00edlia Pedro","family":"Fernandes","sequence":"first","affiliation":[{"name":"Universidade Eduardo Mondlane"}]},{"given":"Pedro","family":"Cravo","sequence":"additional","affiliation":[{"name":"Universidade Nova de Lisboa, 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