{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T22:32:06Z","timestamp":1767825126738,"version":"3.49.0"},"reference-count":32,"publisher":"FapUNIFESP (SciELO)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pesq. agropec. bras."],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p>The objective of this work was to determine the geographic origin of the Madeiran common bean (Phaseolus vulgaris) gene pool. Phaseolin patterns of 50 accessions representing the diversity of common bean collected in Madeira, Portugal, and conserved in the ISOPlexis Germplasm Bank, were analysed using the Experion automated electrophoresis system, based on lab-on-a-chip technology. Five common bean standard varieties with typical phaseolin patterns were used to determine the phytogeographical origin of the Madeiran common bean accessions. Ninety two percent of the accessions exhibited a phaseolin pattern consistent with the one of common bean types belonging to the Andean gene pool, while the origin of the remaining 8% of the accessions was indistinguishable. The application of a similarity coefficient of 85%, based on Pearson correlations, increases the number of accessions with uncertain pattern. The analytical approach used permitted the determination of the origin of the common bean gene pool, which is Andean in 98% of the cases, and clustering of the observed variability among the Madeiran common beans.<\/jats:p>","DOI":"10.1590\/s0100-204x2010000800012","type":"journal-article","created":{"date-parts":[[2010,10,6]],"date-time":"2010-10-06T21:03:15Z","timestamp":1286398995000},"page":"863-871","source":"Crossref","is-referenced-by-count":5,"title":["Phytogeographical origin of Madeiran common beans based on phaseolin patterns"],"prefix":"10.1590","volume":"45","author":[{"given":"Emanuel Marques da","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade da Madeira, Portugal"}]},{"given":"An\u00edsia Soraia Abreu","family":"Correia","sequence":"additional","affiliation":[{"name":"Universidade da Madeira, Portugal"}]},{"given":"Nuno Alexandre Amaral","family":"Lopes","sequence":"additional","affiliation":[{"name":"Universidade da Madeira, Portugal"}]},{"given":"Humberto Gil Moreira","family":"N\u00f3brega","sequence":"additional","affiliation":[{"name":"Universidade da Madeira, Portugal"}]},{"given":"Jos\u00e9 Filipe Teixeira","family":"Ganan\u00e7a","sequence":"additional","affiliation":[{"name":"Universidade da Madeira, Portugal"}]},{"given":"Ana Maria","family":"Domingues","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o Cient\u00edfica Tropical, Portugal"}]},{"given":"Manhaz","family":"Khadem","sequence":"additional","affiliation":[{"name":"Universidade da Madeira, Portugal"}]},{"given":"Jan Jacek","family":"Slaski","sequence":"additional","affiliation":[{"name":"Alberta Innovates, Canada"}]},{"given":"Miguel \u00c2ngelo Almeida Pinheiro de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Universidade da Madeira, Portugal"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1590\/S0100-41582003000300001","article-title":"Coevoluci\u00f3n de interacciones hospedante-pat\u00f3geno en frijol","volume":"28","author":"ARAYA C.M","year":"2003","journal-title":"Fitopatologia Brasileira","ISSN":"https:\/\/id.crossref.org\/issn\/0100-4158","issn-type":"print"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"264","DOI":"10.2135\/cropsci2000.401264x","article-title":"Structure of genetic diversity among common bean landraces of Middle American origin based on correspondence analysis of RAPD","volume":"40","author":"BEEBE E","year":"2000","journal-title":"Crop Science"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1023\/A:1008621632680","article-title":"Wild-weed-crop complexes of common bean (Phaseolus vulgaris L., Fabaceae) in the Andes of Peru and Colombia, and their implications for conservation and, breeding","volume":"44","author":"BEEBE S","year":"1997","journal-title":"Genetic Resources and Crop Evolution"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1365\/s10337-008-0545-2","article-title":"Comparison of the results of SDS PAGE and chip electrophoresis of wheat storage proteins","volume":"67","author":"BRADOV\u00c1 J","year":"2008","journal-title":"Chromatographia","ISSN":"https:\/\/id.crossref.org\/issn\/0009-5893","issn-type":"print"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1023\/A:1024146710611","article-title":"Beans (Phaseolus spp.): model food legumes","volume":"252","author":"BROUGHTON W.J","year":"2003","journal-title":"Plant Soil","ISSN":"https:\/\/id.crossref.org\/issn\/0032-079X","issn-type":"print"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s10722-007-9230-9","article-title":"Evaluation of the maize (Zea mays L.) diversity on the Archipelago of Madeira","volume":"55","author":"CARVALHO M.\u00c2.A.P. de","year":"2008","journal-title":"Genetic Resourses and Crop Evolution"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.2135\/cropsci2004.0501","article-title":"Morphological and molecular characterization of common bean landraces and cultivars from the caribbean","volume":"45","author":"DUR\u00c1N L.A","year":"2005","journal-title":"Crop Science"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1439-0523.1998.tb01447.x","article-title":"Patterns of genetic diversity in landraces of common bean (Phaseolus vulgaris L.) from Galicia","volume":"117","author":"ESCRIBANO M.R","year":"1998","journal-title":"Plant Breeding"},{"key":"ref9","series-title":"Taxonomy, distribution, and ecology of the genus Phaseolus (Leguminosae-Papilinoideae) in North America, Mexico, and Central America","volume":"23","author":"FREYTAG G.F","year":"2002"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/BF02859038","article-title":"Dissemination paths of common bean (Phaseolus vulgaris Fabaceae) deduced from phaseolin electrophoretic variability: II. Europe and Africa","volume":"42","author":"GEPTS P","year":"1988","journal-title":"Economic Botany","ISSN":"https:\/\/id.crossref.org\/issn\/0013-0001","issn-type":"print"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/BF02859659","article-title":"Phaseolinprotein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): evidence for multiple centers of domestication","volume":"40","author":"GEPTS P","year":"1986","journal-title":"Economic Botany","ISSN":"https:\/\/id.crossref.org\/issn\/0013-0001","issn-type":"print"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"9254","DOI":"10.1021\/jf0612981","article-title":"Kaempferol in red and pinto bean seed (Phaseolus vulgaris L.). coats inhibits iron bioavailability using an in vitro digestion\/human caco-2 cell model","volume":"54","author":"HU Y","year":"2006","journal-title":"Journal of Agriculture and Food Chemistry"},{"key":"ref13","series-title":"International rules for seed testing","volume-title":"Verification of species\/cultivar","year":"2004"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1007\/BF00266663","article-title":"Allozyme diversity in wild Phaseolus vulgaris: further evidence for two major centers of genetic diversity","volume":"78","author":"KOENIG R","year":"1989","journal-title":"Theorotical and Applied Genetics"},{"key":"ref15","series-title":"Advances in legume systematics part 1","first-page":"301","volume-title":"Tribe 10: Phaseoleae DC, 1825","author":"LACKEY J.A","year":"1981"},{"key":"ref16","first-page":"317","article-title":"Geographical variation of phaseolin patterns in an old world collection of Phaseolus vulgaris","volume":"17","author":"LIOI L","year":"1989","journal-title":"Seed Science and Technolology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1007\/s10722-006-9185-2","article-title":"Analysis of the contribution of Mesoamerican and Andean gene pools to European common bean (Phaseolus vulgaris L.) germplasm and strategies to establish a core collection","volume":"54","author":"LOGOZZO G","year":"2007","journal-title":"Genetic Resources and Crop Evolution"},{"key":"ref18","series-title":"Performance comparison of the experionTM automated electrophoresis system and competing automated system for protein analysis, Rev A","author":"NGUYEN M","year":"2005"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/BF02310681","article-title":"Extensive introgression of Middle American germplasm into Chilean common bean cultivars","volume":"42","author":"PAREDES O.M","year":"1995","journal-title":"Genetic Resources and Crop Evolution"},{"key":"ref20","first-page":"79","article-title":"Diversity in common bean landraces from south Brazil","volume":"68","author":"PEREIRA T","year":"2009","journal-title":"Acta Botanica Croatica"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1023\/A:1012248002436","article-title":"Diversity of common bean (Phaseolus vulgaris L.) germplasm from Portugal","volume":"48","author":"RODI\u00d1O A.P","year":"2001","journal-title":"Genetic Resourses and Crop Evolution"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1007\/s10722-008-9371-5","article-title":"Morphological characterization of wheat genetic resources from the Island of Madeira, Portugal","volume":"56","author":"SANTOS T.M.M. dos","year":"2009","journal-title":"Genetic Resourses and Crop Evolution"},{"key":"ref23","series-title":"Elucid\u00e1rio madeirense","author":"SILVA F.A","year":"1984"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"807","DOI":"10.4141\/cjps95-136","article-title":"Potential of wild common bean for seed yield improvement of cultivars in the tropics","volume":"75","author":"SINGH S.P","year":"1995","journal-title":"Canadian Journal of Plant Science","ISSN":"https:\/\/id.crossref.org\/issn\/0008-4220","issn-type":"print"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2135\/cropsci1991.0011183X003100010004x","article-title":"Genetic diversity in cultivated common bean: I. 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