{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T05:29:59Z","timestamp":1739338199956,"version":"3.37.0"},"reference-count":36,"publisher":"FapUNIFESP (SciELO)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pesq. Vet. Bras."],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:p>A paratuberculose \u00e9 uma enterite cr\u00f4nica granulomatosa causada por Mycobacterium avium subsp. paratuberculosis que afeta principalmente os ruminantes. A cultura de bact\u00e9rias a partir de amostras de fezes e tecidos constitui um dos m\u00e9todos mais eficazes de diagn\u00f3stico, sendo ainda o \u00fanico m\u00e9todo dispon\u00edvel para obten\u00e7\u00e3o de isolamentos e estirpes de micobact\u00e9rias. Contudo, este m\u00e9todo apresenta baixa sensibilidade e requer meses de incuba\u00e7\u00e3o antes do crescimento de col\u00f4nias. Neste estudo, utilizou-se a cultura fecal como m\u00e9todo de diagn\u00f3stico em ovinos de diferentes ra\u00e7as portuguesas, com sinais compat\u00edveis com a doen\u00e7a. Fez-se ainda a compara\u00e7\u00e3o entre os meios de cultura L\u00f6wenstein Jensen\u00ae com micobactina\u00ae J e o de Middlebrook\u00ae 7H11 com OADC\u00ae, utilizados no isolamento da bact\u00e9ria. As percentagens de isolamento em cada um os meios foram de 2,0% (6\/300) para L\u00f6wenstein Jensen\u00ae com micobactina J e 1,0% (3\/300) para Middlebrook\u00ae 7H11\/OADC. As tr\u00eas amostras positivas no meio de Middlebrook\u00ae 7H11\/OADC tamb\u00e9m foram positivas no meio de L\u00f6wenstein Jensen\u00ae com micobactina J e nenhuma foi somente positiva no meio de Middlebrook\u00ae 7H11\/OADC. Os resultados deste estudo sugerem que o meio de L\u00f6wenstein-Jensen\u00ae com micobactina\u00ae J \u00e9 mais efetivo para a obten\u00e7\u00e3o de estirpes ovinas em Portugal.<\/jats:p>","DOI":"10.1590\/s0100-736x2009000500010","type":"journal-article","created":{"date-parts":[[2009,8,13]],"date-time":"2009-08-13T05:58:55Z","timestamp":1250143135000},"page":"415-420","source":"Crossref","is-referenced-by-count":0,"title":["Compara\u00e7\u00e3o de duas t\u00e9cnicas de isolamento do Mycobacterium avium subsp. paratuberculosis em amostras de fezes de ovinos com suspeita cl\u00ednica de paratuberculose"],"prefix":"10.1590","volume":"29","author":[{"given":"Ana Cl\u00e1udia","family":"Coelho","sequence":"first","affiliation":[{"name":"Universidade de Tr\u00e1s-os-Montes e Alto Douro; CECAV, Portugal"}]},{"given":"Maria de Lurdes","family":"Pinto","sequence":"additional","affiliation":[{"name":"Universidade de Tr\u00e1s-os-Montes e Alto Douro; CECAV, Portugal"}]},{"given":"Adosinda Maria","family":"Coelho","sequence":"additional","affiliation":[{"name":"Divis\u00e3o de Interven\u00e7\u00e3o Veterin\u00e1ria de Vila Real, Portugal"}]},{"given":"Jorge","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Universidade de Tr\u00e1s-os-Montes e Alto Douro; CECAV, Portugal"}]}],"member":"530","reference":[{"year":"1993","author":"Ad\u00fariz J.J.","series-title":"Epidemiologia, diagnostico y control de la paratuberculosis ovina en la Comunidad Autonoma del Pais Vasco","key":"ref1"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0378-1135(95)00037-B","article-title":"Lack of mycobactin dependence of mycobacteria isolated on Middlebrook 7H11 from clinical cases of ovine paratuberculosis","volume":"45","author":"Ad\u00fariz J.J.","year":"1995","journal-title":"Vet. 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