{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T08:51:31Z","timestamp":1768726291102,"version":"3.49.0"},"reference-count":45,"publisher":"FapUNIFESP (SciELO)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Bras. Med. Vet. Zootec."],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p>Three methods for the analysis of cell proliferation, mitotic index\/10 high-power fields (10 HPF), mitotic index\/four sets of 10 HPF (40 HPF), and MIB-1 index were evaluated in a series of canine mammary gland tumors, as well as the possible correlation between them. Fifty-six canine mammary gland tumors, including 23 benign and 33 malignant, were studied. In addition, the prognostic impact of mitotic index\/10 HPF, and histological malignancy grade were evaluated in 17 malignant tumors, being seven ductal and 10 metaplastic carcinomas. The three methods used to evaluate cell proliferation were correlated with the prognostic impact of mitotic index\/10 HPF and histological malignancy grade. The results showed a strong association between mitotic figure counts and MIB-1 index (P&lt;0.0001). A correlation was observed between mitotic count per 40 HPF and MIB-1, and between mitotic index per 10 HPF and 40 HPF (P&lt;0.05). Moreover, histological malignancy grade and mitotic figure counts were excellent prognostic factors during three-year follow-up (P&lt;0.05). There was a correlation between the three methods used for the evaluation of cell proliferation and prognostic factors as observed in human breast cancer studies.<\/jats:p>","DOI":"10.1590\/s0102-09352008000600015","type":"journal-article","created":{"date-parts":[[2009,2,11]],"date-time":"2009-02-11T13:14:00Z","timestamp":1234358040000},"page":"1403-1412","source":"Crossref","is-referenced-by-count":27,"title":["Assessment of cell proliferation and prognostic factors in canine mammary gland tumors"],"prefix":"10.1590","volume":"60","author":[{"given":"A.P.","family":"Dutra","sequence":"first","affiliation":[{"name":"UFMG"}]},{"given":"G.M.","family":"Azevedo J\u00fanior","sequence":"additional","affiliation":[{"name":"UFMG"}]},{"given":"F.C.","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Universidade do Porto Porto,  Portugal"}]},{"given":"G.D.","family":"Cassali","sequence":"additional","affiliation":[{"name":"UFMG"}]}],"member":"530","reference":[{"key":"ref1","first-page":"589","article-title":"So-called mixed tumors of the mammary gland of dog and man, with special reference to the general problem gland of dog and man bone formation","volume":"29","author":"ALLEN A.C","year":"1940","journal-title":"Arch. Pathol."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1002\/1097-0142(19891101)64:9<1901::AID-CNCR2820640924>3.0.CO;2-W","article-title":"Immunohistochemical estrogen receptor determination in paraffin-embedded tissue","volume":"64","author":"ANDERSEN J.","year":"1989","journal-title":"Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref3","first-page":"683","article-title":"Cellular proliferation in breast ductal infiltrating carcinoma","volume":"193","author":"ARANDA F.I.","year":"1997","journal-title":"Correlation with clinical and histopathological variables. Pathol. Res. Pract."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/0046-8177(90)90026-2","article-title":"Mitosis count in tumors","volume":"21","author":"BAAK J.P.A","year":"1990","journal-title":"Hum. Pathol."},{"key":"ref5","first-page":"359","article-title":"Histological grading and prognosis in breast cancer","volume":"9","author":"BLOOM H.J.G.","year":"1957","journal-title":"A study of 1409 cases of which 359 have been followed for 15 years. Br. J. Cancer"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/0169-2607(88)90086-7","article-title":"Microcomputer-assisted univariate survival data analysis using Kaplan-Meier life table estimators","volume":"27","author":"CAMPOS-FILHO N.","year":"1990","journal-title":"Comput. Meth. Prog. Biomed."},{"key":"ref7","series-title":"Estudo morfol\u00f3gico, imuno-histoqu\u00edmico e citom\u00e9trico de tumores mam\u00e1rios da cadela Aspectos comparativos com neoplasias da mama humana","author":"CASSALI G.D.","year":"2000"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1354\/vp.36-6-601","article-title":"Secretory carcinoma of the canine mammary gland","volume":"36","author":"CASSALI G.D.","year":"1999","journal-title":"Vet. Pathol."},{"key":"ref9","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1590\/S0102-09352001000300010","article-title":"A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies","volume":"53","author":"CASSALI G.D.","year":"2001","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref10","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1590\/S0102-09352002000400006","article-title":"Invasive micropapillary carcinoma of the dog mammary gland: a case report","volume":"54","author":"CASSALI G.D.","year":"2002","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1590\/S0102-09352007000500011","article-title":"DNA flow cytometry of canine mammary tumors: comparative aspects with human breast tumors","volume":"59","author":"CASSALI G.D.","year":"2007","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1097\/00000478-199802000-00006","article-title":"Metaplastic carcinoma of the breast with osteocartilaginous heterologous elements","volume":"22","author":"CHHIENG C.","year":"1998","journal-title":"Am. J. Surg. Pathol."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1002\/1097-0142(19930301)71:5<1780::AID-CNCR2820710512>3.0.CO;2-2","article-title":"Pathologic correlates of prognosis in lymph node positive breast carcinomas","volume":"71","author":"CLAYTON F.","year":"1993","journal-title":"Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/j.1365-2559.1991.tb00229.x","article-title":"Pathological prognostic factors in breast cancer: experience from a large study with long-term follow-up","volume":"19","author":"ELSTON C.W.","year":"1991","journal-title":"Histopathology","ISSN":"https:\/\/id.crossref.org\/issn\/0309-0167","issn-type":"print"},{"key":"ref15","series-title":"The breast","first-page":"365","author":"ELSTON C.W.","year":"1998"},{"key":"ref16","first-page":"1311","article-title":"The biological behavior of canine mammary neoplasms","volume":"151","author":"FIDLER I.J.","year":"1967","journal-title":"J. Am. Vet."},{"key":"ref17","series-title":"Statistical methods for rates and proportions","first-page":"212","author":"FLEISS J.L.","year":"1981"},{"key":"ref18","first-page":"1091","article-title":"The histological grading of neoplasms","volume":"112","author":"HENSON D.E","year":"1988","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref19","first-page":"2142","article-title":"Relation among outcome, stage of disease and histologic grade for 22616 cases of breast cancer","volume":"68","author":"HENSON D.E.","year":"1991","journal-title":"The basis for prognostic index. Cancer"},{"key":"ref20","first-page":"1078","article-title":"Metaplastic breast carcinoma","volume":"1","author":"HUVOS A.G.","year":"1973","journal-title":"Rare form of mammary cancer. N. Y. St. J. Med."},{"key":"ref21","first-page":"1180","article-title":"Evaluation of cell proliferation in breast carcinoma","volume":"65","author":"ISOLA J.J.","year":"1990","journal-title":"Comparison of Ki-67 immunohistochemical study, DNA flow cytometry analysis and mitotic count. Cancer"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1002\/1097-0142(19840501)53:9<1908::AID-CNCR2820530917>3.0.CO;2-F","article-title":"Carcinoma of the breast with pseudosarcomatous metaplasia","volume":"53","author":"KAUFMAN M.W.","year":"1984","journal-title":"Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"LANDIS J.R.","year":"1977","journal-title":"Biometrics","ISSN":"https:\/\/id.crossref.org\/issn\/0006-341X","issn-type":"print"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1002\/1097-0142(19891101)64:9<1914::AID-CNCR2820640926>3.0.CO;2-G","article-title":"Prognostic value of histologic grade nuclear components of Scarff-Bloom-Richardson (SBR): An improvement score modification based on a multivariate analysis of 1262 invasive ductal breast carcinoma","volume":"64","author":"LE DOUSSAL V.","year":"1989","journal-title":"Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref25","first-page":"229","article-title":"L'\u00e2ge du chien et celui del'homine","volume":"26","author":"LEBEAU A","year":"1953","journal-title":"Essai de statistique sur la mortalite canine. Bull. Acad. Vet. France"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1016\/S0046-8177(99)90062-X","article-title":"Assessment of proliferative activity in breast cancer: MIB-1 immunohistochemistry versus mitotic figure count","volume":"30","author":"LEHR H.A.","year":"1999","journal-title":"Hum. Pathol."},{"key":"ref27","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/1097-0142(19870101)59:1<83::AID-CNCR2820590119>3.0.CO;2-I","article-title":"The correlation of grow fractions with histologic grading and lymph node status in human mammary carcinoma","volume":"59","author":"LELL\u00c9 R.J.","year":"1987","journal-title":"Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref28","first-page":"4","article-title":"Clinical application of morphologic and immunocytochemical assessments of cell proliferation","volume":"97, suppl. 1","author":"LINDEN M.D.","year":"1992","journal-title":"Am. J. Clin. Pathol."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1177\/030098589703400306","article-title":"Characterization of the proliferation state canine mammary tumors by standardized AgNOR method with post fixation and immunohistologic detection of ki-67 and PCNA","volume":"34","author":"L\u00d6HR C.V.","year":"1997","journal-title":"Vet. Pathol."},{"key":"ref30","first-page":"19","article-title":"Detec\u00e7\u00e3o de receptores de estrog\u00eanio e progesterona e do \u00edndice de prolifera\u00e7\u00e3o em citologia aspirativa de cancros de mama avan\u00e7ado","volume":"11","author":"MARINHO A.","year":"1997","journal-title":"Arq. Med."},{"key":"ref31","series-title":"Histological classification of mammary tumors of the dog and the cat.","author":"MISDORP W.","year":"1999"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1177\/030098587000700401","article-title":"Canine mammary tumors","volume":"7","author":"MOULTON J.E.","year":"1970","journal-title":"Pathol. Vet."},{"key":"ref33","doi-asserted-by":"crossref","first-page":"2819","DOI":"10.1002\/1097-0142(19800615)45:12<2917::AID-CNCR2820451203>3.0.CO;2-M","article-title":"Management and survival of female breast cancer: results of a national survey by American college of Surgeons","volume":"45","author":"NEMOTO T.","year":"1980","journal-title":"Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1177\/104063879801000303","article-title":"Immunohistochemical detection of Ki-67 and PCNA in canine mammary tumors: relationship to clinical and pathologic variables","volume":"10","author":"PE\u00d1A L.L.","year":"1998","journal-title":"J. Vet. Diag. Invest."},{"key":"ref35","first-page":"1514","article-title":"Estrogen receptor immunocytochemistry in paraffin embedded tissues with ER1D5 predicts breast cancer response more accurately than H22sp in frozen sections or cytosol ligand-binding assays","volume":"77","author":"PERTSCHUCK L.P.","year":"1996","journal-title":"Cancer","ISSN":"https:\/\/id.crossref.org\/issn\/0008-543X","issn-type":"print"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/path.1711600202","article-title":"The clinical assessment of proliferation and growth in human tumors: evaluation of methods and application as prognostic variables","volume":"160","author":"QUINN C.M.","year":"1990","journal-title":"J. Pathol.","ISSN":"https:\/\/id.crossref.org\/issn\/0022-3417","issn-type":"print"},{"key":"ref37","series-title":"Tumors of the mammary gland","first-page":"157","author":"ROSEN P.P.","year":"1993"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1016\/S0046-8177(98)90248-9","article-title":"Correlation between mitotic and Ki-67 labeling indices in paraffin-embedded carcinoma specimens","volume":"29","author":"RUDOLPH P.","year":"1998","journal-title":"Hum. Pathol."},{"key":"ref39","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3109\/10520299909066480","article-title":"Evaluating mitotic activity in canine and feline solid tumors: standardizing the parameter","volume":"74","author":"SARLI G.","year":"1999","journal-title":"Biot. Histoch."},{"key":"ref40","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1177\/106689699500200405","article-title":"MIB-1 is a suitable marker of proliferative activity in formalin-fixed, paraffin-embedded sections of breast cancer","volume":"2","author":"SCHMITT F.C.","year":"1995","journal-title":"Int. J. Surg. Pathol."},{"key":"ref41","first-page":"31","article-title":"Factores predictivos en la terapia del carcinoma de mama","volume":"13","author":"SCHMITT F.C.","year":"2000","journal-title":"Rev. Senol. Patol. Mam."},{"key":"ref42","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3109\/07357900009012210","article-title":"Spontaneously occurring tumors of companion animals as models for human breast cancer","volume":"18","author":"VAIL D.M.","year":"2000","journal-title":"Cancer Invest."},{"key":"ref43","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/0046-8177(92)90313-R","article-title":"Reproducibility of mitosis counting in 2469 breast cancer specimens: Results from the multicenter morphometric mammary carcinoma project","volume":"23","author":"VAN DIEST J.P.","year":"1992","journal-title":"Hum. Pathol."},{"key":"ref44","first-page":"628","article-title":"Metaplastic carcinoma of the breast","volume":"20","author":"WARGOTZ E.S.","year":"1989","journal-title":"I. Matrix-producing carcinoma. Hum. Pathol."},{"key":"ref45","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0046-8177(94)90140-6","article-title":"Correlation of Ki-67 antigen expression with mitotic figure index and tumor grade in breast carcinomas using the novel \"paraffin\"-reactive MIB-1 antibody","volume":"25","author":"WEIDNER N.","year":"1994","journal-title":"Hum. Pathol."}],"container-title":["Arquivo Brasileiro de Medicina Veterin\u00e1ria e Zootecnia"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/abmvz\/v60n6\/15.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T01:42:59Z","timestamp":1495762979000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S0102-09352008000600015&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["S0102-09352008000600015"],"URL":"https:\/\/doi.org\/10.1590\/s0102-09352008000600015","relation":{},"ISSN":["0102-0935"],"issn-type":[{"value":"0102-0935","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}