{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:15:12Z","timestamp":1760044512264},"reference-count":34,"publisher":"FapUNIFESP (SciELO)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Bras. Med. Vet. Zootec."],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:p>Tr\u00eas garanh\u00f5es foram utilizados para estudar o efeito da adi\u00e7\u00e3o de trolox e pentoxifilina na motilidade, integridade do acrossoma e DNA de espermatozoides p\u00f3s-descongela\u00e7\u00e3o. Para congela\u00e7\u00e3o, utilizou-se Tris-gema com glicerol (5%) em m\u00e1quina de congela\u00e7\u00e3o de s\u00eamen. As amostras foram descongeladas a 37\u00baC durante 30 segundos e tratadas com: T1= 150\u00b5L de s\u00eamen + 150\u00b5L de Tris; T2= 150\u00b5L de s\u00eamen + 150\u00b5L de Tris + 120\u00b5M\/mL de trolox; T3= 150\u00b5L de s\u00eamen + 150\u00b5L de Tris + 3,5mM de pentoxifilina e T4= 150\u00b5L de s\u00eamen + 150\u00b5L de Tris + 3,5mM de pentoxifilina + 120\u00b5M\/mL de trolox. Ap\u00f3s 0, 60 e 120 minutos de incuba\u00e7\u00e3o (37\u00baC), as amostras foram analisadas quanto \u00e0 motilidade, vigor, integridade de acrossoma e DNA. N\u00e3o houve diferen\u00e7a (P&gt;0,05) entre tratamentos ap\u00f3s 0 e 60 minutos de incuba\u00e7\u00e3o em todos os par\u00e2metros estudados. Ap\u00f3s 120 minutos de incuba\u00e7\u00e3o, verificou-se maior porcentual (P&lt;0,05) de c\u00e9lulas com motilidade total e progressiva nas amostras do T2. Conclui-se que a adi\u00e7\u00e3o de trolox ap\u00f3s descongela\u00e7\u00e3o do s\u00eamen equino preserva a motilidade total e progressiva dos espermatozoides submetidos \u00e0 incuba\u00e7\u00e3o a 37\u00baC durante 120 minutos.<\/jats:p>","DOI":"10.1590\/s0102-09352009000100007","type":"journal-article","created":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T02:23:54Z","timestamp":1242267834000},"page":"42-49","source":"Crossref","is-referenced-by-count":9,"title":["Efeito da adi\u00e7\u00e3o de trolox e pentoxifilina na motilidade, integridade do acrossoma e do DNA de espermatozoides equinos ap\u00f3s descongela\u00e7\u00e3o"],"prefix":"10.1590","volume":"61","author":[{"given":"K.M.G.","family":"Silva","sequence":"first","affiliation":[{"name":"UFRPE"}]},{"given":"T.A.P.","family":"Moraes","sequence":"additional","affiliation":[{"name":"UFRPE"}]},{"given":"E.C.B.","family":"Silva","sequence":"additional","affiliation":[{"name":"UFRPE"}]},{"given":"S.C.","family":"Gamboa","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico de Coimbra, Portugal"}]},{"given":"M.M.P.","family":"Guerra","sequence":"additional","affiliation":[{"name":"UFRPE"}]}],"member":"530","reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"508","DOI":"10.2460\/ajvr.2001.62.508","article-title":"Generation of rective oxygen species by equine spermatozoa","volume":"62","author":"BALL B.A","year":"2001","journal-title":"Am . 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