{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:06:54Z","timestamp":1773835614513,"version":"3.50.1"},"reference-count":6,"publisher":"FapUNIFESP (SciELO)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Braz. arch. biol. technol."],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>Ink disease is one of the most destructive diseases in Castanea sativa. The most common symptoms are root necrosies and a reduction in root growth, which invariably lead to the death of the trees. Phytophthora cinnamomi is an oomycete associated with this disease whose life cycle develops integrally in the soil. In the present work, was a fragment with 1231bp of the glucan endo-1,3-\u03b2-D-glucosidase gene obtained by amplification, using conserved primers and the full-length gene sequence by flanking this known sequence by asymmetric PCR. This fragment was obtained from genomic DNA of Phytophthora cinnamomi isolated in the European Regions of Castilla-Leon (Spain) and Tr\u00e1s-os-Montes (Portugal) and associated with the ink disease of Castanea sativa Mill.<\/jats:p>","DOI":"10.1590\/s1516-89132010000300003","type":"journal-article","created":{"date-parts":[[2010,6,16]],"date-time":"2010-06-16T20:36:51Z","timestamp":1276720611000},"page":"513-518","source":"Crossref","is-referenced-by-count":16,"title":["Isolation and characterization by asymmetric PCR of the ENDO1 gene for glucan endo-1,3-\u03b2-D-glucosidase in Phytophthora cinnamomi associated with the ink disease of Castanea sativa Mill"],"prefix":"10.1590","volume":"53","author":[{"given":"Sofia","family":"Meirinho","sequence":"first","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha,  Portugal"}]},{"given":"Marisa","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha,  Portugal"}]},{"given":"\u00c1ngel","family":"Dominguez","sequence":"additional","affiliation":[{"name":"Universidad de Salamanca,  Spain"}]},{"given":"Altino","family":"Choupina","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha,  Portugal"}]}],"member":"530","reference":[{"key":"ref1","first-page":"722","article-title":"Nucleotide Sequence of cDNA for a 1,3-Beta-Glucanase Associated with Aluminum Toxicity in Wheat Roots","volume":"109","author":"Cruz-Ortega R.","year":"1995","journal-title":"Plant Physiol","ISSN":"https:\/\/id.crossref.org\/issn\/0032-0889","issn-type":"print"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/S1087-1845(02)00523-6","article-title":"Characterization of 1,3-beta-glucanase and 1,3; 1,4-beta-glucanase genes from Phytophthora infestans","volume":"38","author":"McLeod A.","year":"2003","journal-title":"Fungal Genetics and Biology","ISSN":"https:\/\/id.crossref.org\/issn\/1087-1845","issn-type":"print"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/BF02772805","article-title":"Chromosomal walking of flanking regions from short known sequences in GC-Rich plant genomic DNA","volume":"21","author":"Michiels A.N.","year":"2003","journal-title":"Plant Molecular Reporter"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1046\/j.1365-313X.1995.08030457.x","article-title":"Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junction by termal asymmetric interlaced PCR","volume":"8","author":"Liu Y.G.","year":"1995","journal-title":"The Plant Jounal"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1128\/EC.2.2.191-199.2003","article-title":"Molecular Genetics of Pathogenic Oomycetes","author":"Kamoun S.","year":"2003","journal-title":"Eukaryotic Cell"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.1105\/tpc.10.9.1413","article-title":"\"Resistance of Nicotiana benthamiana to Phytophthora infestans is mediated by the recognition of the elicitor protein INF1\"","volume":"10","author":"Kamoun S.","year":"1998","journal-title":"The Plant Cell"}],"container-title":["Brazilian Archives of Biology and Technology"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/pdf\/babt\/v53n3\/a03v53n3.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T16:07:52Z","timestamp":1495814872000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_arttext&pid=S1516-89132010000300003&lng=en&tlng=en"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6]]},"references-count":6,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["S1516-89132010000300003"],"URL":"https:\/\/doi.org\/10.1590\/s1516-89132010000300003","relation":{},"ISSN":["1516-8913"],"issn-type":[{"value":"1516-8913","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6]]}}}