{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:49:28Z","timestamp":1740160168639,"version":"3.37.3"},"reference-count":41,"publisher":"FapUNIFESP (SciELO)","issue":"1","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Bras. Parasitol. Vet."],"abstract":"<jats:p>Abstract  This work describes the detailed ultrastructural morphology of the phagocyte imprisoning an oyster of Nematopsis (Apicomplexa) found in Crassostrea rhizophorae, in the city of Macei\u00f3 (AL), Brazil. The highly infected hosts had half-open leaflets with weak, slow retraction of the adductor muscles. Variable number of ellipsoid oocytes, either isolated and or clustered, was found between myofibrils of the adductor muscle. Each oocyst was incarcerated in a parasitophorous vacuole of host uninucleated phagocyte. The oocysts were composed of a dense wall containing a uninucleate vermiform sporozoite. The wall of the fine oocysts was composed of homogeneous electron-lucent material formed by three layers of equal thickness, having a circular orifice-micropyle obstructed by the operculum. The oocysts presented ellipsoid morphology with their wall was surrounded by a complex network of numerous microfibrils. Important details of the taxonomic value were visualized such as the ultrastructural organization of the oocyst wall and the organization of the micropyle and operculum, beyond the microfibrils that protrude from the oocyst wall only observed by transmission electron microscopy (TEM) and that may aid in the identification of the species. However, in order to clarify the systematic position of the species reported of the genus Nematopsis, it is important to proceed with genetic analyses.<\/jats:p>","DOI":"10.1590\/s1984-29612019010","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T02:06:47Z","timestamp":1553652407000},"page":"97-104","source":"Crossref","is-referenced-by-count":4,"title":["Ultrastructure of phagocytes and oocysts of Nematopsis sp. (Apicomplexa, Porosporidae) infecting Crassostrea rhizophorae in Northeastern Brazil"],"prefix":"10.1590","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7443-1063","authenticated-orcid":false,"given":"Themis Jesus","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Federal de Alagoas,  Brasil"}]},{"given":"Emerson Carlos","family":"Soares","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alagoas,  Brasil"}]},{"given":"Gra\u00e7a","family":"Casal","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Forma\u00e7\u00e3o Avan\u00e7ada em Ci\u00eancias e Tecnologias da Sa\u00fade,  Portugal; Universidade do Porto,  Portugal"}]},{"given":"S\u00f3nia","family":"Rocha","sequence":"additional","affiliation":[{"name":"Universidade do Porto,  Portugal; Universidade do Porto,  Portugal"}]},{"given":"Elton Lima","family":"Santos","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alagoas,  Brasil"}]},{"given":"Renato","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alagoas,  Brasil"}]},{"given":"Elsa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade do Porto,  Portugal"}]},{"given":"Carlos","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Universidade do Porto,  Portugal; Universidade do Porto,  Portugal"}]}],"member":"530","published-online":{"date-parts":[[2019,3]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.14411\/fp.2012.012","article-title":"Ultrastructural characteristics of Nematopsis sp. oocysts (Apicomplexa: Porosporidae), a parasite of the clam Meretrix meretrix (Veneridae) from the Arabian Gulf, Saudi Arabia","volume":"59","author":"Abdel-Baki AA","year":"2012","journal-title":"Folia Parasitol (Praha)"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3354\/dao014069","article-title":"Fine structure of the Apicomplexa oocyst of Nematopsis sp. of two marine bivalve molluscs","volume":"14","author":"Azevedo C","year":"1992","journal-title":"Dis Aquat Organ"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/S0932-4739(99)80052-2","article-title":"Description of Nematopsis mytella n. sp. (Apicomplexa), parasite of the mussel Mytella guyanensis (Mytelidae) from the Amazon estuary and description of its oocysts","volume":"35","author":"Azevedo C","year":"1999","journal-title":"Eur J Protistol"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/j.1550-7408.2004.tb00548.x","article-title":"Nematopsis gigas n. sp. (Apicomplexa), a parasite of Nerita ascencionis (Gastropoda, Neritidae) from Brazil","volume":"51","author":"Azevedo C","year":"2004","journal-title":"J Eukaryot Microbiol"},{"key":"ref5","first-page":"159","article-title":"Gregarines of the genus Nematopsis (Eugregarinida, Porosporidae) - parasites of the Black Sea invertebrates","volume":"30","author":"Belafastova IP","year":"1996","journal-title":"Parazitologiya"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jip.2009.10.008","article-title":"Parasites of three commercially exploited bivalve mollusc species of the estuarine region of the Cachoeira River (Ilh\u00e9us, Bahia, Brazil)","volume":"103","author":"Boehs G","year":"2010","journal-title":"J Invertebr Pathol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0959-8030(94)90028-0","article-title":"Synopsis of infectious diseases and parasites of commercially exploited shellfish","volume":"4","author":"Bower SM","year":"1994","journal-title":"Annu Rev Fish Dis"},{"key":"ref8","series-title":"Parasitic Protozoa.","first-page":"170","volume-title":"Parasitic Protozoa of Mollusca and Crustacea.","author":"Bradbury PC","year":"1994"},{"issue":"1","key":"ref9","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1590\/S1984-29612013005000007","article-title":"Health assessment of the oyster Crassostrea rhizophorae on the southern coast of Bahia, northeastern Brazil","volume":"22","author":"Brand\u00e3o RP","year":"2013","journal-title":"Rev Bras Parasitol Vet"},{"issue":"1","key":"ref10","first-page":"60","article-title":"Presence of Nematopsis sp. (Protozoa, Apicomplexa) in the oyster, Crassostrea rhizophorae (Guilding, 1828), cultivated in the state of Pernambuco, Brazil","volume":"41","author":"Brito LO","year":"2010","journal-title":"World Aquac"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"157","DOI":"10.3354\/dao042157","article-title":"Monitoring of infections by Protozoa by the genera Nematopsis, Perkinsus and Porospora in the smooth Venus clam Callista chione from the North-Western Adriatic Sea (Italy)","volume":"42","author":"Canestri-Trotti G","year":"2000","journal-title":"Dis Aquat Organ"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1590\/S1519-69842012000300002","article-title":"Parasites in the mangrove mussel Mytella guyanensis (Bivalvia: Mytilidae) in Camamu Bay, Bahia, Brazil","volume":"72","author":"Ceuta LO","year":"2012","journal-title":"Braz J Biol"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s12639-010-0002-7","article-title":"First record of a parasitic septate gregarines (Apicomplexa: Sporozoea) in the shrimp Peneaus monodon in Sundarbans of West Bengal","volume":"34","author":"Chakraborti J","year":"2010","journal-title":"J Parasit Dis"},{"issue":"5","key":"ref14","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1111\/1758-2229.12421","article-title":"Nematopsis temporariae (Gregarinasina, Apicomplexa, Alveolata) is an intracellular infectious agent of tadpole livers","volume":"8","author":"Chambouvet A","year":"2016","journal-title":"Environ Microbiol Rep"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"vii","DOI":"10.1016\/S0065-2881(08)60321-1","article-title":"Marine molluscs as hosts for symbioses with a review of known parasites of commercially important species","volume":"5","author":"Cheng TC","year":"1967","journal-title":"Adv Mar Biol"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1590\/S1984-29612015012","article-title":"Parasites in the mangrove oyster Crassostrea rhizophorae cultivated in the estuary of the Graciosa River in Tapero\u00e1, Bahia","volume":"24","author":"Cova AW","year":"2015","journal-title":"Rev Bras Parasitol Vet"},{"issue":"4","key":"ref17","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1046\/j.1355-557x.2002.00647.x","article-title":"Nematopsis marinus n. sp., a new septate gregarine from cultured penaeoid shrimp Litopenaeus vannamei (Boone), in Ecuador","volume":"33","author":"Jim\u00e9nez R","year":"2002","journal-title":"Aquacult Res"},{"issue":"3","key":"ref18","first-page":"217","article-title":"A case study of protozoan parasite gregarine Nematopsis spp. (Apicomplexa: Sporozoa) infection in mangrove oyster Crassostrea belcheri imported from Thailand","volume":"36","author":"Kua BC","year":"2013","journal-title":"Trop Agricult Sci"},{"key":"ref19","series-title":"Diseases of marine animals: 2. Introduction, Bivalvia to Scaphopoda","first-page":"542","volume-title":"Diseases of Mollusca: Bivalvia.","author":"Lauckner G","year":"1983"},{"key":"ref20","first-page":"120","article-title":"Parasites in the oyster Crassostrea rhizophorae from farmed and natural stocks in the Bay of Camamu, Bahia, northeastern Brazil","volume":"76","author":"Luz MSA","year":"2015","journal-title":"J Parasitol Vector Biol"},{"key":"ref21","first-page":"251","article-title":"Ultrastructural morphology of Nematopsis mytella parasite Apicomplexa of bivalve molluscs of south of Brazil","volume":"46","author":"Magalh\u00e3es A","year":"2006","journal-title":"Rev Cienc Agrar"},{"issue":"3","key":"ref22","doi-asserted-by":"crossref","first-page":"207","DOI":"10.3354\/dao01829","article-title":"Pathogens in Crassostrea ariakensis and other Asian oyster species: implications for non-native oyster introduction to Chesapeake Bay","volume":"77","author":"Moss JA","year":"2007","journal-title":"Dis Aquat Organ"},{"issue":"8","key":"ref23","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1017\/S0025315415000740","article-title":"Seasonality and host-parasite interrelationship of Mytilus galloprovincialis parasites in Turkish Sea coast","volume":"95","author":"Ozer A","year":"2015","journal-title":"J Mar Biol"},{"key":"ref24","first-page":"121","article-title":"Fine structure of the oocyst of Nematopsis mytella (Apicomplexa, Porosporidae), a parasite of the mussel Mytella falcata and of the oyster Crassostrea rhizophorae (Mollusca, Bivalvia) from the northeastern Atlantic coast of Brazil","volume":"20","author":"Padovan IP","year":"2003","journal-title":"Braz J Morphol Sci"},{"key":"ref25","series-title":"Microscopic Anatomy of Invertebrates","first-page":"261","volume-title":"\u201cSporozoa\u201d: Apicomplexa, Microsporidia, Haplosporidia, Paramyxea, Myxosporidia, and Actinosporidia","author":"Perkins FO","year":"1991"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"95","DOI":"10.11606\/issn.1678-4456.bjvras.2008.26705","article-title":"Nematopsis sp. 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(Apicomplexa: Cephalina) from the brackishwater prawn, Macrobrachium rosenbergii (De Man)","volume":"137","author":"Shanavas KR","year":"1989","journal-title":"Arch Protistenkd"},{"issue":"5","key":"ref36","first-page":"157","article-title":"Nematopsis spp. Schneider, 1892 (Apicomplexa: Gregarinida) in bivalve molluscs off Ria de Vigo (Galicia, NW Spain)","volume":"16","author":"Soto M","year":"1996","journal-title":"Bull Eur Assoc Fish Pathol"},{"key":"ref37","series-title":"A symposium on diseases of fishes and shellfishes","first-page":"511","volume-title":"Some protozoan parasites and hyperparasites in marine bivalve molluscs.","author":"Sprague V","year":"1970"},{"issue":"1","key":"ref38","doi-asserted-by":"crossref","first-page":"29","DOI":"10.6024\/jmbai.2016.58.1.1890-04","article-title":"Nematopsis sp. 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