{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T04:18:39Z","timestamp":1777522719282,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The Cleft Palate Craniofacial Journal"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the prevalence of mutations in transforming growth factor beta 3 (TGF\u03b23) and Jagged2 genes and their association with nonsyndromic cleft lip with or without cleft palate (CL\u00b1P) patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>Cross-sectional study on nonsyndromic CL\u00b1P and noncleft patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Reconstructive clinic and outpatient dental clinic, Hospital Universiti Sains Malaysia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients<\/jats:title>\n                    <jats:p>Blood samples of 96 nonsyndromic CL\u00b1P and 96 noncleft subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Outcome Measure<\/jats:title>\n                    <jats:p>Prevalence and association of mutations in TGF\u03b23 and Jagged2 genes with nonsyndromic CL\u00b1P.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Most of the nonsyndromic CL\u00b1P patients (53.1%) had left unilateral CLP. There were slightly more females (56.6%) compared with males. The prevalence of the mutations in the TGF\u03b23 gene was 17.7% (95% confidence interval [CI]: 9.5, 24.5) and in the Jagged2 gene was 12.5% (95% CI: 5.5, 18.5), which was higher compared with the noncleft group. For the TGF\u03b23 gene, there was no mutation in the coding region in either of the groups. All variants were single nucleotide polymorphisms located within the intronic flanking region. Two variants were identified (g.15812T&gt;G and g.15966A&gt;G) in both nonsyndromic CL\u00b1P and noncleft patients. However, the association was not significant ( P &gt; .05). Three variants (g.19779C&gt;T, g.19547G&gt;A, and g.19712C&gt;T) were identified in the Jagged2 gene among nonsyndromic CL\u00b1P and noncleft patients. Only g.19712C&gt;T showed a significant association with nonsyndromic CL\u00b1P patients ( P = .039).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>g.19712C&gt;T might play a crucial role in the development of cleft lip and palate. To the best of our knowledge, this is the first report of the mutation found within intron 13 of the Jagged2 gene among nonsyndromic CL\u00b1P Malay patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1597\/14-024","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T17:29:44Z","timestamp":1436290184000},"page":"88-94","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Screening of Transforming Growth Factor Beta 3 and Jagged2 Genes in the Malay Population with Nonsyndromic Cleft Lip with or without Cleft Palate"],"prefix":"10.1177","volume":"52","author":[{"given":"Norliana","family":"Ghazali","sequence":"first","affiliation":[{"name":"Dental Public Health Unit, Universiti Sains Malaysia, Kelantan, Malaysia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Normastura Abd","family":"Rahman","sequence":"additional","affiliation":[{"name":"Dental Public Health Unit, Universiti Sains Malaysia, Kelantan, Malaysia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thirumulu Ponnuraj","family":"Kannan","sequence":"additional","affiliation":[{"name":"Molecular Biology Unit, Universiti Sains Malaysia, Kelantan, Malaysia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saidi","family":"Jaafar","sequence":"additional","affiliation":[{"name":"Molecular Biology Unit, School of Dental Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,7,1]]},"reference":[{"key":"bibr1-14-024","doi-asserted-by":"publisher","DOI":"10.1038\/ng.580"},{"key":"bibr2-14-024","doi-asserted-by":"publisher","DOI":"10.1597\/12-135"},{"key":"bibr3-14-024","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(01)02626-9"},{"key":"bibr4-14-024","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(05)80111-3"},{"key":"bibr5-14-024","doi-asserted-by":"publisher","DOI":"10.1097\/00007611-199504000-00011"},{"key":"bibr6-14-024","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2933"},{"key":"bibr7-14-024","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1048803"},{"key":"bibr8-14-024","doi-asserted-by":"publisher","DOI":"10.1093\/ortho.26.2.115"},{"key":"bibr9-14-024","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1093\/oso\/9780195139068.003.0013","volume-title":"Cleft Lip & Palate: From Origin to 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