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A nomogram for CHD prediction was constructed and validated via calibration curves and ROC curves. Finally, analyses related to functional enrichment, cell infiltration, T cell state score (TCSS) evaluation, regulatory network construction, drug prediction, and molecular docking were performed for key genes. Initially, the 48 candidate genes were identified, followed by the determination of two key genes (BST2 and XPC). A nomogram integrating BST2 and XPC showed high prediction performance. Functional enrichment analysis indicated their involvement in oxidative phosphorylation, spliceosome, and other processes. Notably, CHD patients exhibited altered infiltration of CD8+ effector memory T cells and chondrocytes, with imbalanced TCSS in Quiescence and Terminal Exhaustion T cell subsets. Regulatory network analysis identified transcription factors (e.g., GATA1, and SP1), microRNAs (e.g., hsa-miR-942-5p), and long non-coding RNAs (e.g., H19) associated with key genes. Potential drugs interacting with key genes (e.g., alvespimycin, MG-262) were predicted, though molecular docking revealed weak binding ability. Collectively, these findings provided valuable insights into the molecular mechanisms of CHD, offering a theoretical basis for understanding the disease and developing targeted therapeutic strategies.<\/p>","DOI":"10.1615\/critreveukaryotgeneexpr.2026063041","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T19:48:59Z","timestamp":1772221739000},"page":"13-25","source":"Crossref","is-referenced-by-count":0,"title":["Exploration of Potential Molecular Mechanisms and Key Genes Related to T-Cell Exhaustion in Coronary Heart Disease from a Bioinformatics Perspective"],"prefix":"10.1615","volume":"36","author":[{"given":"Fei","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"613","reference":[{"key":"2","doi-asserted-by":"crossref","unstructured":"Shaya GE, Leucker TM, Jones SR, Martin SS, Toth PP. 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