{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T16:33:33Z","timestamp":1758040413896,"version":"3.44.0"},"reference-count":25,"publisher":"Begell House","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Crit Rev Immunol"],"published-print":{"date-parts":[[2025]]},"abstract":"<p>&lt;b&gt;Objective:&lt;\/b&gt; This study aimed to probe the role of Shenling Baizhu powder (SLBZP) in inhibiting breast cancer (BC) lung metastasis, focusing on epithelial-to-mesenchymal transition (EMT) and ferroptosis.\n\n&lt;b&gt;Methods:&lt;\/b&gt; BC 4T1 cells were treated with low (3.13 &amp;#181;g\/mL) and high (12.5 &amp;#181;g\/mL) doses of SLBZP. Cell proliferation, migration, and invasion were assessed via CCK-8 and transwell assays. Intracellular reactive oxygen species (ROS), malondialdehyde (MDA), and Fe&lt;sup&gt;2&amp;#43;&lt;\/sup&gt; levels were measured using commercial kits. Western blot was used to detect EMT markers (E-cadherin, N-cadherin, Vimentin). In vivo, Balb\/c mice injected with 4T1 cells received SLBZP or cyclophosphamide\n(CTX). Tumor volume, mass, and lung metastases were recorded. MDA, Fe&lt;sup&gt;2&amp;#43;&lt;\/sup&gt;, EMT markers, and ferroptosis-related\nGPX4 were evaluated in tumor tissues.\n\n&lt;b&gt;Results:&lt;\/b&gt; SLBZP dose-dependently suppressed 4T1 cell proliferation, migration, invasion, and EMT, as indicated by upregulated E-cadherin and downregulated N-cadherin and Vimentin. SLBZP increased cellular ROS, MDA, and Fe&lt;sup&gt;2&amp;#43;&lt;\/sup&gt; levels (&lt;i&gt;P&lt;\/i&gt; &amp;#60; 0.05). In vivo, SLBZP and CTX significantly reduced tumor burden and lung metastases, elevated MDA, Fe&lt;sup&gt;2&amp;#43;&lt;\/sup&gt;, and E-cadherin, and decreased N-cadherin, Vimentin, and GPX4 in tumor tissues (&lt;i&gt;P&lt;\/i&gt; &amp;#60; 0.05).\n\n&lt;b&gt;Conclusion:&lt;\/b&gt; SLBZP inhibits BC lung metastasis by modulating EMT and ferroptosis, highlighting its therapeutic potential.<\/p>","DOI":"10.1615\/critrevimmunol.2025059697","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T10:08:47Z","timestamp":1750846127000},"page":"11-18","source":"Crossref","is-referenced-by-count":0,"title":["Shenling Baizhu Powder Inhibits Lung Metastasis of Breast Cancer via Regulation of Epithelial-Mesenchymal Transition and Ferroptosis"],"prefix":"10.1615","volume":"45","author":[{"given":"Wei","family":"Tian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junquan","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"613","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"Barzaman K, Karami J, Zarei Z, Hosseinzadeh A, Kazemi MH, Moradi-Kalbolandi S, Safari E, Farahmand L. 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