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Gastric cancer (GC) remains a significant worldwide health issue, Dapper Antagonist of &beta;-Catenin-1 (<i>DACT1<\/i>) is a gene implicated in various cancers. Bioinformatics analysis across multiple databases were utilized to investigate <i>DACT1<\/i> expression and its correlation with GC prognosis. Subsequent experimental work involved cell culture, Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), and Western Blot to explore the role of <i>DACT1<\/i> in GC cell lines. Bioinformatics analysis revealed that <i>DACT1 <\/i>was highly expressed and linked to a low overall survival rate in GC. <i>DACT1 <\/i>expression was linked to epithelial mesenchymal transition (EMT) and Notch signaling pathways, both critical in cancer progression. Experimental validation further demonstrated that the knockdown of <i>DACT1<\/i> inhibits GC cell proliferation, migration, the EMT process, and the activity of the Notch signaling pathway. <i>DACT1<\/i> may serve as a promising target in GC treatment through its involvement in EMT and Notch signaling. The study provides a foundation for further investigation of <i>DACT1<\/i> in GC.<\/p>","DOI":"10.1615\/jenvironpatholtoxicoloncol.2025054852","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T14:38:58Z","timestamp":1752158338000},"page":"55-70","source":"Crossref","is-referenced-by-count":0,"title":["DACT1 as a Potential Therapeutic Target in Gastric Cancer: Insights from Integrative Bioinformatics and Experimental Analysis"],"prefix":"10.1615","volume":"45","author":[{"given":"Rui-Sheng","family":"Ke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-ling","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun-Zhai","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu-Xing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao-Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"613","reference":[{"key":"2","doi-asserted-by":"crossref","unstructured":"Richman DM, Tirumani SH, Hornick JL, Fuchs CS, Howard S, Krajewski K, Ramaiya N, Rosenthal M. 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