{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:48:44Z","timestamp":1791413324067,"version":"4.3.3"},"reference-count":30,"publisher":"Tohoku University Medical Press","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tohoku J. Exp. Med."],"published-print":{"date-parts":[[2015]]},"DOI":"10.1620\/tjem.237.45","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T18:03:39Z","timestamp":1441217019000},"page":"45-50","source":"Crossref","is-referenced-by-count":24,"title":["A Synthetic Serine Protease Inhibitor, Nafamostat Mesilate, Is a Drug Potentially Applicable to the Treatment of Ebola Virus Disease"],"prefix":"10.1620","volume":"237","author":[{"given":"Hidekazu","family":"Nishimura","sequence":"first","affiliation":[{"name":"Virus Research Center, Clinical Research Division, Sendai Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mutsuo","family":"Yamaya","sequence":"additional","affiliation":[{"name":"Department of Advanced Preventive Medicine for Infectious Disease, Tohoku University Graduate School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"622","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"Aoyama, T., Ino, Y., Ozeki, M., Oda, M., Sato, T., Koshiyama, Y., Suzuki, S. &amp; Fujita, M. (1984) Pharmacological studies of FUT-175, nafamstat mesilate. I. Inhibition of protease activity in in vitro and in vivo experiments. <i>Jpn. J. Pharmacol<\/i>., 35, 203-227.","DOI":"10.1254\/jjp.35.203"},{"key":"2","doi-asserted-by":"crossref","unstructured":"Bray, M., Hatfill, S., Hensley, L. &amp; Huggins, J.W. (2001) Haematological, biochemical and coagulation changes in mice, guinea-pigs and monkeys infected with a mouse-adapted variant of Ebola Zaire virus. <i>J. Comp. Pathol<\/i>., 125, 243-253.","DOI":"10.1053\/jcpa.2001.0503"},{"key":"3","unstructured":"Centers for Disease Control and Prevention (2015) Ebola virus disease (EVD) information for clinicians in U.S. healthcare settings. http:\/\/www.cdc.gov\/vhf\/ebola\/hcp\/clinician-information-us-healthcare-settings.html [<i>Accessed<\/i>: January 30, 2015]."},{"key":"4","doi-asserted-by":"crossref","unstructured":"Chandran, K., Sullivan, N.J., Felbor, U., Whelan, S.P. &amp; Cunningham, J.M. (2005) Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection. <i>Science<\/i>, 308, 1643-1645.","DOI":"10.1126\/science.1110656"},{"key":"5","unstructured":"Feldmann, H., Sanchez, A. &amp; Geisbert, T.W. (2013) Filoviridae: Marburg and Ebola viruses. Chapter 32. In <i>Fields Virology<\/i>, 6th ed., edited by Knipe, D.M. &amp; Howley, P.M. Lippincott Williams &amp; Wilkins, Philadelphia, PA, pp. 923-956."},{"key":"6","doi-asserted-by":"crossref","unstructured":"Fujii, S. &amp; Hitomi, Y. (1981) New synthetic inhibitors of C1r, C1 esterase, thrombin, plasmin, kallikrein and trypsin. <i>Biochim. Biophys. Acta<\/i>., 661, 342-345.","DOI":"10.1016\/0005-2744(81)90023-1"},{"key":"7","doi-asserted-by":"crossref","unstructured":"Geisbert, T.W., Hensley, L.E., Jahrling, P.B., Larsen, T., Geisbert, J.B., Paragas, J., Young, H.A., Fredeking, T.M., Rote, W.E. &amp; Vlasuk, G.P. (2003) Treatment of Ebola virus infection with a recombinant inhibitor of factor VIIa\/tissue factor: a study in rhesus monkeys. <i>Lancet<\/i>, 362, 1953-1958.","DOI":"10.1016\/S0140-6736(03)15012-X"},{"key":"8","doi-asserted-by":"crossref","unstructured":"Hitomi, Y., Ikari, N. &amp; Fujii, S. (1985) Inhibitory effect of a new synthetic protease inhibitor (FUT-175) on the coagulation system. <i>Haemostasis<\/i>, 15, 164-168.","DOI":"10.1159\/000215139"},{"key":"9","doi-asserted-by":"crossref","unstructured":"Hofmann-Winkler, H., Kaup, F. &amp; P\u00f6hlmann, S. (2012) Host cell factors in filovirus entry: novel players, new insights. <i>Viruses<\/i>, 4, 3336-3362.","DOI":"10.3390\/v4123336"},{"key":"10","doi-asserted-by":"crossref","unstructured":"Hunt, C.L., Lennemann, N.J. &amp; Maury, W. (2012) Filovirus entry: a novelty in the viral fusion world. <i>Viruses<\/i>, 4, 258-275.","DOI":"10.3390\/v4020258"},{"key":"11","unstructured":"Japanese Society of Hospital Pharmacists (2015) Medical drugs interview form Futhan10 and Futhan5. Medical interview form 4th edition. http:\/\/www.torii.co.jp\/iyakuDB\/data\/if\/if_fut.pdf [<i>Accessed<\/i>: July 16, 2015]."},{"key":"12","doi-asserted-by":"crossref","unstructured":"Koshiyama, Y., Kobori, A., Ogihara, M., Yokomoto, Y., Ohtani, K., Shimamura, K. &amp; Iwaki, M. (1987) The effects of FUT-175 (nafamostat mesilate) on blood coagulation and experimental disseminated intravascular coagulation (DIC). <i>Nihon Yakurigaku Zasshi<\/i>, 90, 313-320 (in Japanese, abstract in English).","DOI":"10.1254\/fpj.90.313"},{"key":"13","doi-asserted-by":"crossref","unstructured":"Koshiyama, Y., Ozeki, M., Motoyoshi, A., Fujita, M., Iwaki, M. &amp; Aoyama, T. (1984) Pharmacological studies of FUT-175, nafamstat mesilate. IV. Effects on coagulation, platelets and fibrinolysis. <i>Nihon Yakurigaku Zasshi<\/i>, 84, 417-428 (in Japanese, abstract in English).","DOI":"10.1254\/fpj.84.417"},{"key":"14","unstructured":"Manabe, T., Hirano, T., Imanishi, K., Ando, K., Yotsumoto, F. &amp; Tobe, T. (1992) Protective effect of nafamostat mesilate on cellular and lysosomal fragility of acinar cells in rat cerulein pancreatitis. <i>Int. J. Pancreatol<\/i>., 12, 167-172."},{"key":"15","doi-asserted-by":"crossref","unstructured":"Marzi, A. &amp; Falzarano, D. (2015) An updated Ebola vaccine: immunogenic, but will it protect? <i>Lancet<\/i>, 385, 2229-2230.","DOI":"10.1016\/S0140-6736(15)60613-4"},{"key":"16","doi-asserted-by":"crossref","unstructured":"Marzi, A., Reinheckel, T. &amp; Feldmann, H. (2012) Cathepsin B &amp; L are not required for ebola virus replication. <i>PLoS. Negl. Trop. Dis<\/i>., 6, e1923.","DOI":"10.1371\/journal.pntd.0001923"},{"key":"17","doi-asserted-by":"crossref","unstructured":"Mohammadi, D. (2014) First trials for Ebola treatments announced. <i>Lancet<\/i>, 384, 1883.","DOI":"10.1016\/S0140-6736(14)62043-2"},{"key":"18","doi-asserted-by":"crossref","unstructured":"Muto, S., Imai, M. &amp; Asano, Y. (1994) Mechanisms of the hyperkalaemia caused by nafamostat mesilate: effects of its two metabolites on Na<sup>+<\/sup> and K<sup>+<\/sup> transport properties in the rabbit cortical collecting duct. <i>Br. J. Pharmacol<\/i>., 111, 173-178.","DOI":"10.1111\/j.1476-5381.1994.tb14040.x"},{"key":"19","unstructured":"Nanpo, T., Ohtsuki, T., Jin, Y., Matsunaga, K., Takahashi, M, Shibuya, M., Sasaki, H. &amp; Kurumi, M. (1984) Pharmakokinetic studies of FUT-175 (Nafamostat mesilate) (1) Blood level profiles, tissue distribution, metabolism and excretion in rats after intravenous administration. <i>Kiso to Rinsho<\/i>, 18, 3971-3992 (in Japanese, abstract in English)."},{"key":"20","unstructured":"Okamoto, K., Takeda, S. &amp; Ohsato, K. (1991) Treatment of disseminated intravascular coagulation with proteinase inhibitor. <i>Nihon Rinsho<\/i>, 49, 2130-2135 (in Japanese)."},{"key":"21","unstructured":"Pak, K., Shirasu, A., Okino, K., Sako, H., Nakane, Y., Umemura, K., Maeda, S. &amp; Tomoyoshi, T. (1988) Effectiveness of FUT-175, protease inhibitor, as an anticoagulant to hemodialysis. <i>Hinyokika Kiyo<\/i>, 34, 1077-1081 (in Japanese, abstract in English)."},{"key":"22","unstructured":"Shibata, A., Takahashi, H., Aoki, N., Matsuda, T., Yasunaga, K., Kondoh, M., Matsuda, M., Hattori, A., Kobayashi, N., Ogawa, N., Kawakami, Y., Miyazaki, T., Hirayama, A., Yoshida, Y., Chiba, Y., et al. (1988) Clinical effects of FUT-175 (nafamostat mesilate) on disseminated intravascular coagulation (DIC): multi institutional clinical study. <i>Rinsho to Kenkyu<\/i>, 65, 921-940 (in Japanese)."},{"key":"23","unstructured":"Shibata, A., Takahashi, H., Hattori, A., Kawakami, Y., Hasegawa, H., Miyazaki, T., Morioka, M., Hirayama, A., Uehara, S., Yoshida, Y., Aihara, M., Chiba, Y., Miura, A., Mamiya, S., Goto, Y., et al. (1987) Clinical effects of nafamostat mesilate (FUT-175) on DIC: multi institutional later phase II clinical study. <i>Rinsho to Kenkyu<\/i>, 64, 1887-1900 (in Japanese)."},{"key":"24","doi-asserted-by":"crossref","unstructured":"Takemoto, Y., Tanaka, S., Tanabe, J., Nakamura, Y., Kohama, A. &amp; Shibata, S. (1986) Studies on the effects of primary therapy for DIC following circulatory arrest. <i>Am. J. Hematol<\/i>., 21, 377-382.","DOI":"10.1002\/ajh.2830210406"},{"key":"25","unstructured":"Toya, D., Hattori, N. &amp; Sawabu, N. (1984) Effects of FUT-175 on pancreatitis. <i>Kiso to Rinsho<\/i>, 18, 3006-3012 (in Japanese)."},{"key":"26","unstructured":"Tsukagoshi, S. (2000) Pharmacokinetics studies of nafamostat mesilate (FUT), a synthetic protease inhibitor, which has been used for the treatments of DIC and acute pancreatitis, and as an anticoagulant in extracorporeal circulation. <i>Gan To Kagaku Ryoho<\/i>, 27, 767-774 (in Japanese, abstract in English)."},{"key":"27","doi-asserted-by":"crossref","unstructured":"Uchiba, M., Okajima, K., Abe, H., Okabe, H. &amp; Takatsuki, K. (1994) Effect of nafamostat mesilate, a synthetic protease inhibitor, on tissue factor-factor VIIa complex activity. <i>Thromb. Res<\/i>., 74, 155-161.","DOI":"10.1016\/0049-3848(94)90008-6"},{"key":"28","doi-asserted-by":"crossref","unstructured":"Wong, A.C., Sandesara, R.G., Mulherkar, N., Whelan, S.P. &amp; Chandran, K. (2010) A forward genetic strategy reveals destabilizing mutations in the Ebolavirus glycoprotein that alter its protease dependence during cell entry. <i>J. Virol<\/i>., 84, 163-175.","DOI":"10.1128\/JVI.01832-09"},{"key":"29","unstructured":"World Health Organization (2014) Consultation on potential Ebola therapies and vaccines. http:\/\/www.who.int\/mediacentre\/events\/meetings\/2014\/ebola-interventions\/en\/ [<i>Accessed<\/i>: August 7, 2015]."},{"key":"30","doi-asserted-by":"crossref","unstructured":"Yoshikawa, T., Murakami, M., Furukawa, Y., Kato, H., Takemura, S. &amp; Kondo, M. (1983) Effects of FUT-175, a new synthetic protease inhibitor on endotoxin-induced disseminated intravascular coagulation in rats. <i>Haemostasis<\/i>, 13, 374-378.","DOI":"10.1159\/000214825"}],"container-title":["The Tohoku Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/tjem\/237\/1\/237_45\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T14:49:37Z","timestamp":1498229377000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/tjem\/237\/1\/237_45\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015]]}},"URL":"https:\/\/doi.org\/10.1620\/tjem.237.45","relation":{},"ISSN":["0040-8727","1349-3329"],"issn-type":[{"value":"0040-8727","type":"print"},{"value":"1349-3329","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}