{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:25:05Z","timestamp":1791120305828,"version":"4.1.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"NIH","award":["HL071303"],"award-info":[{"award-number":["HL071303"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The epithelium of the mouse tongue and soft palate consists of at least three distinct epithelial cell populations: basal cells, keratinized cells organized into filiform and fungiform papillae, and taste receptor cells present in tight clusters known as taste buds in the fungiform and circumvallate papillae and soft palate. All three cell types develop from the simple epithelium of the embryonic tongue and palate, and are continually replaced in the adult by cell turnover. Previous studies using pulse-chase tritiated thymidine labeling in the adult mouse provided evidence for a high rate of cell turnover in the keratinocytes (5\u20137 days) and taste buds (10 days). However, little is known about the localization and phenotype of the long-term stem or progenitor cells that give rise to the mature taste bud cells and surrounding keratinocytes in these gustatory tissues. Here, we make use of a tamoxifen-inducible K14-CreER transgene and the ROSA26 LacZ reporter allele to lineage trace the mature keratinocytes and taste bud cells of the early postnatal and adult mouse tongue and soft palate. Our results support the hypothesis that both the pore keratinocytes and receptor cells of the taste bud are derived from a common K14+K5+Trp63+Sox2+ population of bipotential progenitor cells located outside the taste bud. The results are also compatible with models in which the keratinocytes of the filiform and fungiform papillae are derived from basal progenitor cells localized at the base of these structures.<\/jats:p>","DOI":"10.1634\/stemcells.2008-0611","type":"journal-article","created":{"date-parts":[[2008,11,27]],"date-time":"2008-11-27T02:55:27Z","timestamp":1227754527000},"page":"442-450","source":"Crossref","is-referenced-by-count":198,"title":["Cell Lineage Mapping of Taste Bud Cells and Keratinocytes in the Mouse Tongue and Soft Palate"],"prefix":"10.1093","volume":"27","author":[{"given":"Tadashi","family":"Okubo","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"Center for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigid L.M.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,2,2]]},"reference":[{"key":"2022011221095257100_bib1","doi-asserted-by":"crossref","first-page":"1916","DOI":"10.1073\/pnas.92.6.1916","article-title":"Taste receptor cells arise from local epithelium, not neurogenic ectoderm","volume":"92","author":"Stone","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2022011221095257100_bib2","doi-asserted-by":"crossref","first-page":"4522","DOI":"10.1523\/JNEUROSCI.22-11-04522.2002","article-title":"Analysis of cell lineage relationships in taste buds","volume":"22","author":"Stone","year":"2002","journal-title":"J Neurosci"},{"key":"2022011221095257100_bib3","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/0012-1606(65)90040-0","article-title":"Electron microscope study of the developing taste bud in rat fungiform papilla","volume":"11","author":"Farbman","year":"1965","journal-title":"Dev Biol"},{"key":"2022011221095257100_bib4","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/aja.1001430303","article-title":"Morphogenesis of rat lingual filiform papillae","volume":"143","author":"Baratz","year":"1975","journal-title":"Am J Anat"},{"key":"2022011221095257100_bib5","doi-asserted-by":"crossref","first-page":"2654","DOI":"10.1101\/gad.1457106","article-title":"Sox2 is required for development of taste bud sensory cells","volume":"20","author":"Okubo","year":"2006","journal-title":"Genes Dev"},{"key":"2022011221095257100_bib6","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s00441-008-0600-1","article-title":"Expression of Sox2 in mouse taste buds and its relation to innervation","volume":"332","author":"Suzuki","year":"2008","journal-title":"Cell Tissue Res"},{"key":"2022011221095257100_bib7","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1016\/j.mod.2004.07.002","article-title":"Pax9 is required for filiform papilla development and suppresses skin-specific differentiation of the mammalian tongue epithelium","volume":"121","author":"Jonker","year":"2004","journal-title":"Mech Dev"},{"key":"2022011221095257100_bib8","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1038\/19531","article-title":"p63 is a p53 homologue required for limb and epidermal morphogenesis","volume":"398","author":"Mills","year":"1999","journal-title":"Nature"},{"key":"2022011221095257100_bib9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1242\/jcs.22.1.149","article-title":"The ordered columnar structure of mouse filiform papillae","volume":"22","author":"Hume","year":"1976","journal-title":"J Cell Sci"},{"key":"2022011221095257100_bib10","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1083\/jcb.27.2.263","article-title":"Renewal of cells within taste buds","volume":"27","author":"Beidler","year":"1965","journal-title":"J Cell Biol"},{"key":"2022011221095257100_bib11","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/cne.902530210","article-title":"Ultrastructure of mouse vallate taste buds: II. 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