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Thus, as indicated by small interfering RNA experiments, the transient early expression of Lmx1a is necessary for the coordinated expression of all other dopamine (DA)-specific phenotypic traits as hES cells move from multipotent human neural progenitor cells (hNPs) to more restricted precursor cells in vitro. Moreover, only Lmx1a-specified hNPs have the potential to differentiate into bona fide mDA neurons after transplantation into the 6-hydroxydopamine-treated rat striatum. In contrast, cortical human neuronal precursor cells (HNPCs) and mouse subventricular zone cells do not express Lmx1a or become mDA neurons even when placed in an environment that fosters their DA differentiation in vitro or in vivo. These findings suggest that Lmx1a may be critical to the development of mDA neurons from hES cells and that, along with other key early DA markers (i.e., Aldh1a1), may prove to be extremely useful for the selection of appropriately staged and suitably mDA-specified hES cells for cell replacement in Parkinson's disease.<\/jats:p>","DOI":"10.1634\/stemcells.2008-0734","type":"journal-article","created":{"date-parts":[[2008,10,3]],"date-time":"2008-10-03T00:44:12Z","timestamp":1222994652000},"page":"220-229","source":"Crossref","is-referenced-by-count":69,"title":["The Role of Lmx1a in the Differentiation of Human Embryonic Stem Cells into Midbrain Dopamine Neurons in Culture and After Transplantation into a Parkinson's Disease Model"],"prefix":"10.1093","volume":"27","author":[{"given":"Jingli","family":"Cai","sequence":"first","affiliation":[{"name":"Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Donaldson","sequence":"additional","affiliation":[{"name":"Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[{"name":"Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"German","sequence":"additional","affiliation":[{"name":"University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grigori","family":"Enikolopov","sequence":"additional","affiliation":[{"name":"Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 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