{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:35:03Z","timestamp":1793439303677,"version":"4.5.0"},"reference-count":26,"publisher":"American Association of Avian Pathologists (AAAP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Avian Diseases"],"published-print":{"date-parts":[[2021,5,17]]},"abstract":"<jats:p>Avian erythroblastosis (AE; erythroid leukosis) was detected in a 78-day-old Japanese native chicken. At necropsy, the liver was enlarged and diffusely dark red in color. Moderate splenomegaly was observed. Histologically, round to polygonal tumor cells were observed only in the blood vessels of the liver and other organs. Immunohistochemistry (IHC) was performed on formalin-fixed, paraffin-embedded (FFPE) sections to characterize tumor cells. Tumor cells, as well as normal erythrocytes as positive controls, were consistently positive for IHC by using the commercially available anti-human hemoglobin antibody. Exogenous avian leukosis virus (ALV) subgroup B and endogenous ALV-E genes were detected by PCR, although ultrastructural observation revealed no viral particles associated with tumor cells. Results suggest that the commercial anti-human hemoglobin antibody used in the study cross-react to chicken erythrocytes on FFPE sections by IHC and can also be used for definitive diagnosis of the spontaneous case of AE in chickens.<\/jats:p>","DOI":"10.1637\/0005-2086-65.3.381","type":"journal-article","created":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T02:15:35Z","timestamp":1627870535000},"page":"381-384","source":"Crossref","is-referenced-by-count":1,"title":["Spontaneous Avian Erythroblastosis in a Chicken Confirmed by Immunohistochemical Detection of Hemoglobin in Tumor Cells"],"prefix":"10.1637","volume":"65","author":[{"given":"Seiji","family":"Fujiki","sequence":"first","affiliation":[{"name":"Gifu Prefectural Chuo Livestock Hygiene Service Center, 1-1 Yanagito, Gifu 501-1112, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoi","family":"Kurokawa","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1212","reference":[{"key":"1","unstructured":"HJ Barnes  and OJ Fletcher  \"Avian histopathology.\"  \"Hemic system.\" 1 (2016)"},{"key":"2","doi-asserted-by":"crossref","unstructured":"T Graf  and H Beug  \"Biochim Biophys Acta.\"  516, \"Avian leukemia viruses interaction with their target cells in vivo and in vitro.\" 269 (1978)","DOI":"10.1016\/0304-419X(78)90011-2"},{"key":"3","unstructured":"MJ Hayman  and H Beug  \"Cancer Surv.\"  15, \"Avian erythroblastosis: a model system to study oncogene co-operation in leukemia.\" 53 (1992)"},{"key":"4","doi-asserted-by":"crossref","unstructured":"H Beug, A von Kirchbach, G D\u00f6derlein, et al.  \"Cell.\"  18, \"Chicken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiation.\" 375 (1979)","DOI":"10.1016\/0092-8674(79)90057-6"},{"key":"5","unstructured":"BR Burmester, M Adrian Gross, WG Walter, et al.  \"J Natl Cancer Inst.\"  22, \"Pathogenicity of a viral strain (RPL12) causing avian visceral lymphomatosis and related neoplasms. 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