{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T12:45:28Z","timestamp":1794487528495,"version":"4.9.1"},"reference-count":26,"publisher":"American Association of Avian Pathologists (AAAP)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Avian Diseases"],"published-print":{"date-parts":[[2023,6,5]]},"abstract":"<jats:p>Reporte de caso- Caracterizaci\u00f3n de un brote reciente de Enterococcus cecorum causante de una enfermedad sist\u00e9mica grave simult\u00e1neamente con problemas en las patas en un integrador de pollo de engorde en el sur de los Estados Unidos. La bacteria Enterococcus cecorum se ha asociado principalmente con osteomielitis de la v\u00e9rtebra tor\u00e1cica m\u00f3vil de los pollos. Sin embargo, existen reportes de E. cecorum produciendo lesiones septic\u00e9micas y presentando tropismo por los cart\u00edlagos, resultando en la presentaci\u00f3n de necrosis de la cabeza femoral y sinovitis. Este art\u00edculo analiza la presentaci\u00f3n de E. cecorum relacionada con un brote en un integrador vertical donde las principales lesiones estaban relacionadas con septicemia. Utilizando un m\u00e9todo de muestreo de conveniencia, se analizaron 100 casos de pollos de engorde recibidos en el Laboratorio de Investigaci\u00f3n y Diagn\u00f3stico Av\u00edcolas de la Universidad Estatal de Mississippi de abril a diciembre del 2021. El mayor n\u00famero de casos se observ\u00f3 de junio a agosto. La edad promedio de los pollos de engorde fue de 21 d\u00edas con un rango de 15 a 31 d\u00edas. La mayor\u00eda de estos casos estaban relacionados con enfermedad sist\u00e9mica y problemas en las patas, con lesiones macrosc\u00f3picas que inclu\u00edan pericarditis caracter\u00edstica junto con perihepatitis, osteomielitis y artritis. En seis de los 100 casos, E cecorum se aisl\u00f3 de la v\u00e9rtebra tor\u00e1cica m\u00f3vil, y el resto de los casos se recuper\u00f3 de otros lugares, incluyendo el h\u00edgado, el pericardio, articulaci\u00f3n del corvej\u00f3n, la cabeza femoral y la m\u00e9dula \u00f3sea. La identificaci\u00f3n de E. cecorum se realiz\u00f3 utilizando la plataforma Vitek de espectrometr\u00eda de masas MALDI-TOF. Posteriormente, estos resultados se enviaron a la base de datos SARAMIS de uso exclusivo para investigaci\u00f3n para su an\u00e1lisis. Una vez importados los espectros de los aislados, se analiz\u00f3 la taxonom\u00eda relativa y absoluta. Se identificaron dos superespectros y tres grupos mediante homolog\u00eda. Las concentraciones inhibitorias m\u00ednimas obtenidas mediante pruebas de sensibilidad antimicrobiana se analizaron utilizando el software de base de datos de laboratorio de microbiolog\u00eda de WHONET. Ning\u00fan aislamiento fue pan-susceptible, se observ\u00f3 que el 80% de los aislamientos eran resistentes a tres o m\u00e1s clases de antibi\u00f3ticos y en general, los aislamientos exhibieron un alto grado de variabilidad al examinar los patrones de resistencia a los antimicrobianos.<\/jats:p>","DOI":"10.1637\/aviandiseases-d-22-00085","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T03:47:03Z","timestamp":1685591223000},"page":"137-144","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of an Emerging Enterococcus cecorum Outbreak Causing Severe Systemic Disease with Concurrent Leg Problems in a Broiler Integrator in the Southern United States"],"prefix":"10.1637","volume":"67","author":[{"given":"Gunnar","family":"Dunnam","sequence":"first","affiliation":[{"name":"Mississippi State University, College of Veterinary Medicine, Poultry Research and Diagnostic Laboratory, 39208"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay Kay","family":"Thornton","sequence":"additional","affiliation":[{"name":"Mississippi State University, College of Veterinary Medicine, Poultry Research and Diagnostic Laboratory, 39208"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Pulido-Landinez","sequence":"additional","affiliation":[{"name":"Mississippi State University, College of Veterinary Medicine, Poultry Research and Diagnostic Laboratory, 39208"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1212","reference":[{"key":"17","unstructured":"\"SAS\/ACCESS\u00ae 9.4 Interface to ADABAS: reference.\"  (2013)"},{"key":"21","doi-asserted-by":"crossref","unstructured":"J Pitcovski  and SM Goyal  \"Diseases of poultry.\"  \"Avian reovirus infections.\" 382 (2020)","DOI":"10.1002\/9781119371199.ch11"},{"key":"20","doi-asserted-by":"crossref","unstructured":"JM Sharma, K Karaca, T Pertile,  \"Poult Sci.\"  73, \"Virus-induced immunosuppression in chickens.\" 1082 (1994)","DOI":"10.3382\/ps.0731082"},{"key":"1","doi-asserted-by":"publisher","unstructured":"MJ Stalker, ML Brash, A Weisz, et al.  \"J Vet Diagn Invest.\"  22, \"Arthritis and osteomyelitis associated with Enterococcus cecorum infection in broiler and broiler breeder chickens in Ontario, Canada.\" 643 (2010)","DOI":"10.1177\/104063871002200426"},{"key":"2","doi-asserted-by":"crossref","unstructured":"G. Klein,  \"Int J Food Microbiol.\"  88, \"Taxonomy, ecology, and antibiotic resistance of enterococci from food and the gastro-intestinal tract.\" 123 (2003)","DOI":"10.1016\/S0168-1605(03)00175-2"},{"key":"3","doi-asserted-by":"crossref","unstructured":"A Jung, LR Chen, MM Suyemoto, et al.  \"Avian Dis.\"  62, \"A review of Enterococcus cecorum infection in poultry.\" 261 (2018)","DOI":"10.1637\/11825-030618-Review.1"},{"key":"4","doi-asserted-by":"publisher","unstructured":"LB Borst, M Suyemoto, AH Sarsour, et al.  \"Vet Pathol.\"  54, \"Pathogenesis of enterococcal spondylitis caused by Enterococcus cecorum in broiler chickens.\" 61 (2016)","DOI":"10.1177\/0300985816658098"},{"key":"5","doi-asserted-by":"crossref","unstructured":"B Dolka, D Chrobak-Chmiel, M Czopowicz, et al.  \"PLoS ONE.\"  12, \"Characterization of pathogenic Enterococcus cecorum from different poultry groups: broiler chickens, layers, turkeys, and waterfowl.\" e0185199 (2017)","DOI":"10.1371\/journal.pone.0185199"},{"key":"6","doi-asserted-by":"publisher","unstructured":"B Dolka, D Chrobak-Chmiel, L Makrai, et al.  \"BMC Vet Res.\"  12, \"Phenotypic and genotypic characterization of Enterococcus cecorum strains associated with infections in poultry.\" 129 (2016)","DOI":"10.1186\/s12917-016-0761-1"},{"key":"7","doi-asserted-by":"crossref","unstructured":"MJ Kense  and WJM Landman  \"Avian Pathol.\"  40, \"Enterococcus cecorum infections in broiler breeders and their offspring: molecular epidemiology.\" 603 (2011)","DOI":"10.1080\/03079457.2011.619165"},{"key":"8","doi-asserted-by":"crossref","unstructured":"R Thiemann, J Thornton, P Stayer, et al.  \"Avian Dis.\"  66, \"Novel presentation of Clostridium perfringens in young broilers.\" 1 (2022)","DOI":"10.1637\/aviandiseases-D-21-00107"},{"key":"9","unstructured":"\"National Poultry Improvement Plan Program Standards.\"  (2017)"},{"key":"10","unstructured":"MS. Vitek\u00ae,  \"Knowledge Base Version 2.2. Customer training manual. SARAMIS\u2122 software: SuperSpectra creation and other research functionalities. Software version 3.1.\"  (2020)"},{"key":"11","doi-asserted-by":"crossref","unstructured":"C Leyer, G Gregorowicz, F Mougari, et al.  \"J Clin Microbiol.\"  55, \"Comparison of Saramis 4.12 and IVD 3.0 Vitek MS matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry for identification of mycobacteria from solid and liquid culture media.\" 2045 (2017)","DOI":"10.1128\/JCM.00006-17"},{"key":"12","doi-asserted-by":"publisher","unstructured":"SS Nielsen, DJ Bicout, P Calistri, et al.  \"EFSA J.\"  20, \"Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016\/429): antimicrobial-resistant Enterococcus cecorum in poultry.\" e7126 (2022)","DOI":"10.2903\/j.efsa.2022.7126"},{"key":"13","unstructured":"\"Sensititre SWIN Software System. Catalog number SW1202.\"  (2022)"},{"key":"14","unstructured":"P. Weinstein Melvin,  \"Performance standards for antimicrobial susceptibility testing.\"  2021"},{"key":"15","unstructured":"\"Break points used for susceptibility testing of Enterococcus.\""},{"key":"16","unstructured":"\"Glossary of terms related to antibiotic resistance.\"  (2019)"},{"key":"18","unstructured":"\"WHONET: Windows-based database software for surveillance of antimicrobial resistance, version 22.16.24.\"  (2011)"},{"key":"19","doi-asserted-by":"crossref","unstructured":"M Robbins Kabel, M Mitsu Suyemoto, RL Lyman, et al.  \"Avian Dis.\"  56, \"An outbreak and source investigation of enterococcal spondylitis in broilers caused by Enterococcus cecorum.\" 768 (2012)","DOI":"10.1637\/10253-052412-Case.1"},{"key":"22","doi-asserted-by":"publisher","unstructured":"MD Winfield  and EA Groisman  \"Appl Environ Microb.\"  69, \"Role of nonhost environments in the lifestyles of Salmonella and Escherichia coli.\" 3687 (2003)","DOI":"10.1128\/aem.69.7.3687-3694.2003"},{"key":"23","doi-asserted-by":"crossref","unstructured":"C Jackson, S Kariyawasam, L Borst, et al.  \"Lett Appl Microbiol.\"  60, \"Antimicrobial resistance, virulence determinants and genetic profiles of clinical and nonclinical Enterococcus cecorum from poultry.\" 111 (2015)","DOI":"10.1111\/lam.12374"},{"key":"24","doi-asserted-by":"crossref","unstructured":"JL Mart\u00ednez  and F Baquero  \"Clin Microbiol Rev.\"  15, \"Interactions among strategies associated with bacterial infection: pathogenicity, epidemicity, and antibiotic resistance.\" 647 (2002)","DOI":"10.1128\/CMR.15.4.647-679.2002"},{"key":"25","doi-asserted-by":"crossref","unstructured":"KM Krause, AW Serio, TR Kane, et al.  \"Cold Spring Harb Perspect Med.\"  6, \"Aminoglycosides: an overview.\" a027029 (2016)","DOI":"10.1101\/cshperspect.a027029"},{"key":"26","doi-asserted-by":"crossref","unstructured":"N Ahmadpoor, R Ahmadrajabi, S Esfahani, et al.  \"Med Princ Pract.\"  30, \"High-level resistance to erythromycin and tetracycline and dissemination of resistance determinants among clinical enterococci in Iran.\" 272 (2021)","DOI":"10.1159\/000516216"}],"container-title":["Avian Diseases"],"original-title":[],"link":[{"URL":"https:\/\/bioone.org\/journals\/avian-diseases\/volume-67\/issue-2\/aviandiseases-D-22-00085\/Characterization-of-an-Emerging-Enterococcus-cecorum-Outbreak-Causing-Severe-Systemic\/10.1637\/aviandiseases-D-22-00085.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:51:10Z","timestamp":1793425870000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/avian-diseases\/volume-67\/issue-2\/aviandiseases-D-22-00085\/Characterization-of-an-Emerging-Enterococcus-cecorum-Outbreak-Causing-Severe-Systemic\/10.1637\/aviandiseases-D-22-00085.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,5]]},"references-count":26,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,8,4]]}},"URL":"https:\/\/doi.org\/10.1637\/aviandiseases-d-22-00085","relation":{},"ISSN":["0005-2086","1938-4351"],"issn-type":[{"value":"0005-2086","type":"print"},{"value":"1938-4351","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,5]]}}}