{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:36:20Z","timestamp":1793439380456,"version":"4.5.0"},"reference-count":11,"publisher":"American Association of Zoo Veterinarians","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Zoo and Wildlife Medicine"],"published-print":{"date-parts":[[2021,4,1]]},"abstract":"<jats:p>This study describes the novel use of diffusible iodine-based contrast-enhanced computed tomography (diceCT) as a digital necropsy aid. DiceCT was used postmortem to evaluate the cause of progressive respiratory disease in a juvenile maleo (Macrocephalon maleo). The technique facilitated soft-tissue contrast and a three-dimensional investigation of sinus and choanal anatomy as a means to identify normal and pathologic morphologies. Results showed right-sided narial occlusion by mucoid debris, along with left-sided choanal stenosis caused by osteomyelitis and reactive bone formation. The high spatial resolution afforded by diceCT enabled targeted histology and quantification of the clinical impact of pathologies, which contributed to an effective 60% loss in nasal airway aperture for this individual. This study demonstrates how adding diceCT to traditional necropsy can proffer additional understanding of an individual's pathology, and the resulting data can enhance research programs in vertebrate anatomy, evolution, and health.<\/jats:p>","DOI":"10.1638\/2020-0086","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T10:04:25Z","timestamp":1617357865000},"page":"406-412","source":"Crossref","is-referenced-by-count":1,"title":["DIFFUSIBLE IODINE-BASED CONTRAST-ENHANCED COMPUTED TOMOGRAPHY AS A NECROPSY AID: A CASE REPORT EVALUATING RESPIRATORY DISEASE IN MACROCEPHALON MALEO"],"prefix":"10.1638","volume":"52","author":[{"given":"Paul M.","family":"Gignac","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd L.","family":"Green","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Oehler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Malatos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean A.","family":"Par\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Hollinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1213","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"RWRJ. Dekker,  \"Biol Conser.\"  51, \"The distribution and status of nesting grounds of the maleo, Macrocephalon maleo in Sulawesi, Indonesia.\" 139 (1990)","DOI":"10.1016\/0006-3207(90)90108-2"},{"key":"2","doi-asserted-by":"crossref","unstructured":"PM Gignac  and NJ Kley  \"J Exp Zool B (Mol Dev Evol).\"  322B, \"Iodine-enhanced microCT imaging: Methodological refinements for the study of soft-tissue anatomy of post-embryonic vertebrates.\" 166 (2014)","DOI":"10.1002\/jez.b.22561"},{"key":"3","doi-asserted-by":"crossref","unstructured":"PM Gignac  and NJ Kley  \"Brain Behav Evol.\"  91, \"The utility of diceCT imaging for high-throughput comparative neuroanatomical studies.\" 180 (2018)","DOI":"10.1159\/000485476"},{"key":"4","doi-asserted-by":"crossref","unstructured":"PM Gignac, NJ Kley, JA Clarke, et al.  \"J Anat.\"  228, \"Diffusible iodine-based contrast-enhanced computed tomography (diceCT): an emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues.\" 889 (2016)","DOI":"10.1111\/joa.12449"},{"key":"5","doi-asserted-by":"crossref","unstructured":"ME Gold, D Shulz, M Budassi, et al.  \"Curr Biol.\"  26, \"Flying starlings and PET lend insights on the evolution of volant dinosaurs.\" R265 (2016)","DOI":"10.1016\/j.cub.2016.02.025"},{"key":"6","doi-asserted-by":"crossref","unstructured":"AN Herdina, H Plenk, P Benda, et al.  \"J Morph.\"  276, \"Correlative 3D-imaging of Pipistrellus penis micromorphology: validating quantitative microCT images with undecalcified serial ground section histomorphology.\" 695 (2015)","DOI":"10.1002\/jmor.20372"},{"key":"7","unstructured":"et al.  \"Macrocephalon maleo.\"  , \"The IUCN Red List of Threatened Species.\"  ()"},{"key":"8","unstructured":"et al.  \"The megapodes.\"  ,  (2018)"},{"key":"9","doi-asserted-by":"crossref","unstructured":"T. McNamara,  \"Int Zoo Yb.\"  41, \"The role of zoos in biosurveillance.\" 12 (2007)","DOI":"10.1111\/j.1748-1090.2007.00019.x"},{"key":"10","doi-asserted-by":"crossref","unstructured":"HD O'Brien, PM Gignac, T Hieronymus, et al.  \"PeerJ.\"  4, \"Post-natal growth of the cranial arteries of the giraffe (Artiodactyla: Giraffa camelopardalis).\" e1696 (2016)","DOI":"10.7717\/peerj.1696"},{"key":"11","doi-asserted-by":"crossref","unstructured":"A Watanabe, PM Gignac, TL Green, et al.  \"J Anat.\"  234, \"Brain-endocast dissimilarity through postnatal ontogeny in archosaurs, with recommendations for comparative neuroanatomical studies.\" 291 (2019)","DOI":"10.1111\/joa.12918"}],"container-title":["Journal of Zoo and Wildlife Medicine"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:28:11Z","timestamp":1793438891000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-zoo-and-wildlife-medicine\/volume-52\/issue-1\/2020-0086\/DIFFUSIBLE-IODINE-BASED-CONTRAST-ENHANCED-COMPUTED-TOMOGRAPHY-AS-A-NECROPSY\/10.1638\/2020-0086.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,1]]},"references-count":11,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,3,1]]}},"URL":"https:\/\/doi.org\/10.1638\/2020-0086","relation":{},"ISSN":["1042-7260","1937-2825"],"issn-type":[{"value":"1042-7260","type":"print"},{"value":"1937-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,1]]}}}