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Santo  \"Small Ruminant Research.\"  106, \"Tick-borne diseases in sheep and goats: Clinical and diagnostic aspects.\" S6 (2012)","DOI":"10.1016\/j.smallrumres.2012.04.026"},{"key":"4","unstructured":"A. Ali, M. Ijaz, A. Z. Durrani, et al.  \"Pakistan. Journal of Zoology.\"  46, \"Prevalence and chemotherapy of anaplasmosis in clinically affected small ruminants in the River Ravi Region, Lahore.\" 876 (2014)"},{"key":"5","doi-asserted-by":"crossref","unstructured":"S. Ali, M. Ijaz, A. Ghaffar, et al.  \"Pakistan Veterinary Journal.\"  40, \"Species distribution and seasonal dynamics of equine tick infestation in two subtropical climate niches in Punjab, Pakistan.\" 25 (2020)","DOI":"10.29261\/pakvetj\/2019.095"},{"key":"6","doi-asserted-by":"crossref","unstructured":"M. Azmat, M. Ijaz, S. 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Bevan,  \"Veterinary Journal (1900).\"  68, \"Anaplasmosis of sheep.\" 400 (1912)","DOI":"10.1016\/S0372-5545(17)66040-1"},{"key":"10","doi-asserted-by":"crossref","unstructured":"Z. Bursac, C. H. Gauss, D. K. Williams, et al.  \"Source Code for Biology and Medicine.\"  3, \"Purposeful selection of variables in logistic regression.\"  (2008)","DOI":"10.1186\/1751-0473-3-17"},{"key":"11","doi-asserted-by":"crossref","unstructured":"D. Chochlakis, I. Ioannou, Y. Tselentis, et al.  \"Emerging Infectious Diseases.\"  16, \"Human anaplasmosis and Anaplasma ovis variant.\" 1031 (2010)","DOI":"10.3201\/eid1606.090175"},{"key":"12","doi-asserted-by":"crossref","unstructured":"S. T. De Echaide, M. F. Bono, C. Lugaresi, et al.  \"Veterinary Microbiology.\"  106, \"Detection of antibodies against Anaplasma marginale in milk using a recombinant MSP5 indirect ELISA.\" 287 (2005)","DOI":"10.1016\/j.vetmic.2004.12.026"},{"key":"13","doi-asserted-by":"crossref","unstructured":"M. Derd\u00e1kov\u00e1, A. \u0160tefan\u010d\u00edkov\u00e1, E. \u0160pitalsk\u00e1, et al.  \"Veterinary Microbiology.\"  153, \"Emergence and genetic variability of Anaplasma species in small ruminants and ticks from Central Europe.\" 293 (2011)","DOI":"10.1016\/j.vetmic.2011.05.044"},{"key":"14","unstructured":"et al.  \"Economic Advisor Wing, Islamabad (2018\u20132019).\"  , 27 ()"},{"key":"15","doi-asserted-by":"crossref","unstructured":"S. H. Farooqi, M. Ijaz, M. I. Rashid, et al.  \"Tropical Animal Health and Production.\"  50, \"Molecular epidemiology of bovine anaplasmosis in Khyber Pakhtunkhwa, Pakistan.\" 1591 (2018)","DOI":"10.1007\/s11250-018-1599-2"},{"key":"16","doi-asserted-by":"crossref","unstructured":"D. B. Fishbein, J. E. Dawson, L. E. Robinson,  \"Annals of Internal Medicine.\"  120, \"Human ehrlichiosis in the United States, 1985 to 1990.\" 736 (1994)","DOI":"10.7326\/0003-4819-120-9-199405010-00003"},{"key":"17","doi-asserted-by":"crossref","unstructured":"J. Gomes, R. Soares, M. Santos, et al.  \"Ticks and Tick-Borne Diseases.\"  4, \"Detection of Theileria and Babesia infections amongst asymptomatic cattle in Portugal.\" 148 (2013)","DOI":"10.1016\/j.ttbdis.2012.07.002"},{"key":"18","unstructured":"M. Kashif  and M. S. Ahmad  \"Journal of Zoology Studies.\"  1, \"Geographical seroprevalence of Anaplasma marginale infection (anaplasmosis) by ELISA in Ovis aries, in district Peshawar, Pakistan.\" 15 (2014)"},{"key":"19","unstructured":"A. Khan, M. R. Khan, M. Tufail, et al.  \"Scholar's Advances in Animal and Veterinary Research.\"  2, \"Pathogenesis of anaplasmosis in Bulkhi sheep and their confirmation with polymerase chain reaction.\" 107 (2015)"},{"key":"20","doi-asserted-by":"crossref","unstructured":"A. Khan, R. D. Mitchell, S. Niaz, et al.  \"Small Ruminant Research.\"  176, \"Seroprevalence of Anaplasma spp. among sheep and goats in Charsadda District, Pakistan.\" 5 (2019)","DOI":"10.1016\/j.smallrumres.2019.05.008"},{"key":"21","doi-asserted-by":"crossref","unstructured":"K. M. Kocan, J. De La Fuente, A. A. Guglielmone, et al.  \"Clinical Microbiology Reviews.\"  16, \"Antigens and alternatives for control of Anaplasma marginale infection in cattle.\" 698 (2003)","DOI":"10.1128\/CMR.16.4.698-712.2003"},{"key":"22","doi-asserted-by":"crossref","unstructured":"S. Kumar, G. Stecher, T. Koichiro,  \"Molecular Biology and Evolution.\"  33, \"MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.\" 1870 (2016)","DOI":"10.1093\/molbev\/msw054"},{"key":"23","doi-asserted-by":"crossref","unstructured":"M. Ma, Z. Liu, M. Sun, et al.  \"Journal of Clinical Microbiology.\"  49, \"Development and evaluation of a loop-mediated isothermal amplification method for rapid detection of Anaplasma ovis.\" 2143 (2011)","DOI":"10.1128\/JCM.02536-10"},{"key":"24","unstructured":"K. \u00d6ter, H. Cetinkaya, C. Vuru\u015faner, et al.  \"Kafkas Universitesi Veteriner Fakultesi Dergisi.\"  22, \"Molecular detection and typing of Anaplasma species in small ruminants in Thrace region of Turkey.\" 133 (2016)"},{"key":"25","doi-asserted-by":"crossref","unstructured":"H. Qiu, P. J. Kelly, J. Zhang, et al.  \"Canadian Journal of Infectious Diseases and Medical Microbiology.\"  2016, \"Molecular detection of Anaplasma spp. and Ehrlichia spp. in ruminants from twelve provinces of China.\" 9183861 (2016)","DOI":"10.1155\/2016\/9183861"},{"key":"26","unstructured":"O. M. Radostits, C. C. Gay, K. W. 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Alberti, et al.  \"Vector-Borne and Zoonotic Diseases.\"  15, \"Molecular survey of Anaplasma species in small ruminants reveals the presence of novel strains closely related to A. phagocytophilum in Tunisia.\" 580 (2015)","DOI":"10.1089\/vbz.2015.1796"},{"key":"30","doi-asserted-by":"crossref","unstructured":"S. Saleem, M. Ijaz, S. H. Farooqi, et al.  \"Microbial Pathogenesis.\"  119, \"Equine granulocytic anaplasmosis 28 years later.\" 1 (2018a)","DOI":"10.1016\/j.micpath.2018.04.001"},{"key":"31","doi-asserted-by":"crossref","unstructured":"S. Saleem, M. Ijaz, S. H. Farooqi, et al.  \"Acta Tropica.\"  180, \"First molecular evidence of equine granulocytic anaplasmosis in Pakistan.\" 18 (2018b)","DOI":"10.1016\/j.actatropica.2017.12.032"},{"key":"32","unstructured":"W. Schroeder  and M. Ristic  \"American Journal of Veterinary Research.\"  29, \"Blood serum factors associated with erythrophagocytosis in calves with anaplasmosis.\" 1991 (1968)"},{"key":"33","doi-asserted-by":"crossref","unstructured":"S. Shompole, S. D. Waghela, F. R. Rurangirwa, et al.  \"Journal of Clinical Microbiology.\"  27, \"Cloned DNA probes identify Anaplasma ovis in goats and reveal a high prevalence of infection.\" 2730 (1989)","DOI":"10.1128\/JCM.27.12.2730-2735.1989"},{"key":"34","doi-asserted-by":"crossref","unstructured":"S. Stuen, I. Van De Pol, K. Bergstr\u00f6m, et al.  \"Journal of Clinical Microbiology.\"  40, \"Identification of Anaplasma phagocytophila (formerly Ehrlichia phagocytophila) variants in blood from sheep in Norway.\" 3192 (2002)","DOI":"10.1128\/JCM.40.9.3192-3197.2002"},{"key":"35","doi-asserted-by":"crossref","unstructured":"R. Talat, T. Khanum, A. Hayat,  \"Pakistan Journal of Biological Sciences.\"  8, \"Studies on mammalian haematozoan parasites of NWFP Pakistan.\" 726 (2005)","DOI":"10.3923\/pjbs.2005.726.729"},{"key":"37","doi-asserted-by":"crossref","unstructured":"A. Torina, A. Agnone, V. Blanda, et al.  \"Ticks and Tick-Borne Diseases.\"  3, \"Development and validation of two PCR tests for the detection of and differentiation between Anaplasma ovis and Anaplasma marginale.\" 283 (2012)","DOI":"10.1016\/j.ttbdis.2012.10.033"},{"key":"38","doi-asserted-by":"crossref","unstructured":"A. Torina, R. C. Galindo, J. Vicente, et al.  \"Tropical Animal Health and Production.\"  42, \"Characterization of Anaplasma phagocytophilum and A. ovis infection in a naturally infected sheep flock with poor health condition.\" 1327 (2010)","DOI":"10.1007\/s11250-010-9580-8"},{"key":"39","doi-asserted-by":"crossref","unstructured":"J. Yang, R. Han, Q. Niu, et al.  \"Ticks and Tick-Borne Diseases.\"  9, \"Occurrence of four Anaplasma species with veterinary and public health significance in sheep, northwestern China.\" 82 (2018)","DOI":"10.1016\/j.ttbdis.2017.10.005"},{"key":"40","doi-asserted-by":"crossref","unstructured":"J. Yang, Y. Li, Z. Liu, et al.  \"Parasites & Vectors.\"  8, \"Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China.\" 108 (2015)","DOI":"10.1186\/s13071-015-0727-3"},{"key":"41","unstructured":"S. Yasini, Z. Khaki, S. Rahbari, et al.  \"Iranian Journal of Parasitology.\"  7, \"Hematologic and clinical aspects of experimental ovine anaplasmosis caused by Anaplasma ovis in Iran.\" 91 (2012)"},{"key":"42","doi-asserted-by":"crossref","unstructured":"A. Yousefi, S. Rahbari, P. 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