{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:51:07Z","timestamp":1791247867044,"version":"4.1.0"},"reference-count":24,"publisher":"American Society of Parasitologists","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology"],"published-print":{"date-parts":[[2024,12,20]]},"abstract":"<jats:p>Many technical aspects are associated with helminth egg isolation and enumeration that affect how efficiently eggs are recovered from samples. This study investigated Ascaris egg recoverability when samples were washed with or without pressure, and from different sample types (water, effluent, ventilated improved pit latrine [VIP], urine diversion dry toilet [UDDT], dried, fatty, and septic tank sludges, and soil) when processed with water, ammonium bicarbonate, and 7X\u00ae. We also looked at egg recovery after flotation with zinc sulfate, magnesium sulfate, and sodium nitrate at specific gravities of 1.18, 1.2, and 1.3, at respective centrifugation speeds and times after washing (1,050 and 1,512 g for 5, 10, and 15 min) and after flotation (672 and 1,050 g for 5, 10, and 15 min). We found that samples should be washed under pressure to ensure full dissociation of eggs from the sample matrix and then centrifuged at 1,512 g for 10 min. For sludge samples (or samples with high-fat content), 7X produced the best egg recovery and clearest samples for microscopic analysis, while soil and soil-containing (UDDT sludge) samples were best processed with ammonium bicarbonate. Flotation was optimal with zinc sulfate at a specific gravity of 1.3 after centrifugation at 672 g for 15 min.<\/jats:p>","DOI":"10.1645\/22-59","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T01:22:56Z","timestamp":1735262576000},"page":"717-724","source":"Crossref","is-referenced-by-count":1,"title":["The Effects of Technical Steps Used in Existing Sanitation Helminth Test Methods on Ascaris suum Egg Recovery from Pig Feces"],"prefix":"10.1645","volume":"110","author":[{"given":"D.","family":"Naidoo","sequence":"first","affiliation":[{"name":"Water, Sanitation and Hygiene Research and Development Centre, Howard College Campus, University of KwaZulu-Natal, Durban 4041, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. E.","family":"Archer","sequence":"additional","affiliation":[{"name":"Water, Sanitation and Hygiene Research and Development Centre, Howard College Campus, University of KwaZulu-Natal, Durban 4041, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1220","reference":[{"key":"6","unstructured":"M. W. Dryden, P. A. Payne, R. Ridley, et al.  \"Veterinary Therapeutics.\"  6, \"Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts.\" 15 (2005)"},{"key":"21","doi-asserted-by":"publisher","unstructured":"C. Smith,  \"Transactions of the Royal Society of Tropical Medicine and Hygiene.\"  85, \"Induced larval motility: A possible viability test for embryonated Ascaris lumbricoides ova.\" 760 (1991)","DOI":"10.1016\/0035-9203(91)90447-7"},{"key":"3","doi-asserted-by":"crossref","unstructured":"D. D. Bowman, M. D. Little, R. S. 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Chiodini,  \"PloS Neglected Tropical Diseases.\"  10, \"Faecal parasitology: Concentration methodology needs to be better standardised.\" 4 (2016)","DOI":"10.1371\/journal.pntd.0004579"},{"key":"10","doi-asserted-by":"crossref","unstructured":"D. Naidoo  and C. E. Archer  \"Journal of Parasitology.\"  110, \"Effects of regeants and chemicals used in existing sanitation helminth test methods on the viability of Ascaris suum eggs.\" 709 (2024a)","DOI":"10.1645\/22-58"},{"key":"11","doi-asserted-by":"crossref","unstructured":"D. Naidoo  and C. E. Archer  \"Journal of Parasitology.\"  110, \"Ascaris suum egg recovery from sludge samples after phase extraction.\" 295 (2024b)","DOI":"10.1645\/22-57"},{"key":"12","doi-asserted-by":"crossref","unstructured":"C. M. Nunes, I. L. Sinhorini, S. 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Lindquist  \"Journal of Parasitology.\"  68, \"Determination of the specific gravity of certain helminth eggs using sucrose density gradient centrifugation.\" 916 (1982)","DOI":"10.2307\/3281005"}],"container-title":["Journal of Parasitology"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:53:38Z","timestamp":1791244418000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-parasitology\/volume-110\/issue-6\/22-59\/The-Effects-of-Technical-Steps-Used-in-Existing-Sanitation-Helminth\/10.1645\/22-59.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,20]]},"references-count":24,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,11,1]]}},"URL":"https:\/\/doi.org\/10.1645\/22-59","relation":{},"ISSN":["0022-3395","1937-2345"],"issn-type":[{"value":"0022-3395","type":"print"},{"value":"1937-2345","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,20]]}}}