{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:54:42Z","timestamp":1791248082306,"version":"4.1.0"},"reference-count":43,"publisher":"American Society of Parasitologists","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology"],"published-print":{"date-parts":[[2024,8,13]]},"abstract":"<jats:p>Naegleria fowleri is a protozoan that causes primary amebic meningoencephalitis (PAM). The infection occurs when the trophozoites enter the nasal cavity, adhere to the nasal mucosa, invade the epithelium, and migrate until they reach the olfactory bulb. Like other pathogens, there is evidence that the adhesion of N. fowleri to host cells is an important factor in the process of cytopathogenicity and disease progression. However, the factors involved in the adhesion of the pathogen to the cells of the nasal epithelium have not been characterized. The objective of this study was to identify a protein on the surface of N. fowleri, which could act as adhesin to the mouse nasal epithelium in the PAM model. The interaction between proteins of extracts of N. fowleri and cells of the nasal epithelium of BALB\/c mice was analyzed using overlay and Western blot assays. A 72-kDa band of N. fowleri interacted directly with epithelial cell proteins, this polypeptide band was purified and analyzed by mass spectrometry. Analysis revealed that polypeptide bands of 72 kDa contained peptides that matched the membrane protein, actin 1 and 2, and Hsp70. Moreover, the N. fowleri extracts resolved in 2D-SDS-PAGE showed that 72-kDa spot interacted with proteins of mouse epithelial cells, which include characteristics of the theoretical data of molecular weight and pH obtained in the analysis by mass spectrometry. Immunofluorescence tests showed that this protein is located on the surface of trophozoites and plays an important role in the adhesion of amoeba either in vitro or in vivo assays, suggesting that this protein contributes during the N. fowleri invasion and migration to the brain, causing primary amoebic meningoencephalitis.<\/jats:p>","DOI":"10.1645\/22-86","type":"journal-article","created":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T02:23:04Z","timestamp":1723688584000},"page":"360-374","source":"Crossref","is-referenced-by-count":3,"title":["The 72-KDA Protein of Naegleria fowleri Plays An Important Role in the Adhesion of Trophozoites to BALB\/C Mice Nasal Epithelium"],"prefix":"10.1645","volume":"110","author":[{"given":"B.","family":"Flores-Su\u00e1rez","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Ambiental. Proyecto CyMA, UIICSE, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tlalnepantla, C.P. 54090, Estado de M\u00e9xico, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Bonilla-Lemus","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Ambiental. Proyecto CyMA, UIICSE, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tlalnepantla, C.P. 54090, Estado de M\u00e9xico, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Rojas-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunobiolog\u00eda Molecular y Celular, Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Salvador D\u00edaz Mir\u00f3n esq. Plan de San Luis S\/N, Miguel Hidalgo, Casco de Santo Tomas, Ciudad de M\u00e9xico 11340, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. l.","family":"Terrazas-Vald\u00e9s","sequence":"additional","affiliation":[{"name":"Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tlalnepantla, C.P. 54090, Estado de M\u00e9xico, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6290-3393","authenticated-orcid":false,"given":"M. M.","family":"Carrasco-Y\u00e9pez","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Ambiental. Proyecto CyMA, UIICSE, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tlalnepantla, C.P. 54090, Estado de M\u00e9xico, M\u00e9xico."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1220","reference":[{"key":"1","doi-asserted-by":"publisher","unstructured":"K. Aldape, H. Huizinga, J. Bouvier, et al.  \"Experimental Parasitology.\"  78, \"Naegleria fowleri: Characterization of a secreted histolytic cysteine protease.\" 230 (1994)","DOI":"10.1006\/expr.1994.1023"},{"key":"2","doi-asserted-by":"publisher","unstructured":"M. Carrasco-Yepez, Campos-Rodriguez R., M. Godinezvictoria, et al.  \"Parasitology Research.\"  112, \"Naegleria fowleri glycoconjugates with residues of \u03b1-D-mannose are involved in adherence of trophozoites to mouse nasal mucosa.\" 3615 (2013)","DOI":"10.1007\/s00436-013-3549-2"},{"key":"3","doi-asserted-by":"publisher","unstructured":"M. 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