{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:56:46Z","timestamp":1791248206070,"version":"4.1.0"},"reference-count":25,"publisher":"American Society of Parasitologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology"],"published-print":{"date-parts":[[2024,5,30]]},"abstract":"<jats:p>Alveolar echinococcosis is considered to be one of the most potentially lethal parasitic zoonotic diseases. However, the molecular mechanisms by which Echinococcus multilocularis interacts with hosts are poorly understood, hindering the prevention and treatment of this disease. Due to the great advantages of cell culture systems for molecular research, numerous attempts have been made to establish primary cell cultures for E. multilocularis. In this study we developed a simple, rapid, and economical method that allows E. multilocularis metacestode tissue blocks to generate daughter vesicles without the continuous presence of host feeder cells in a regular medium. We performed anaerobic, hypoxic (1% O2), normoxic, and semi-anaerobic (in sealed tubes) cultures and found that E. multilocularis metacestode tissues can produce daughter vesicles only in the sealed tubes after 4 wk of incubation. The daughter vesicles cultivated in this system were remarkably enlarged under anaerobic conditions after 8 days of culture, whereas vesicles cultured under hypoxic (1% O2) and normoxic conditions showed only a mild increase in volume. Our in vitro cultivated vesicles showed strong viability and could be used to test antiparasitic drugs, isolate primary cells, and infect animals.<\/jats:p>","DOI":"10.1645\/23-100","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T01:47:03Z","timestamp":1717379223000},"page":"210-217","source":"Crossref","is-referenced-by-count":2,"title":["A Simple, Quick, and Economical Method for in Vitro Cultivation of Echinococcus multilocularis Metacestode and Generation of Primary Cells"],"prefix":"10.1645","volume":"110","author":[{"given":"Cuiying","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihua","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Fu","sequence":"additional","affiliation":[{"name":"Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Xining, Qinghai 810000, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Hou","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7138-0993","authenticated-orcid":false,"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1220","reference":[{"key":"3","doi-asserted-by":"crossref","unstructured":"P. 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Guo, et al.  \"Parasites & Vectors.\"  9, \"In vitro culture of Echinococcus multilocularis producing protoscoleces and mouse infection with the cultured vesicles.\" 411 (2016)","DOI":"10.1186\/s13071-016-1687-y"}],"container-title":["Journal of Parasitology"],"original-title":[],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:39:22Z","timestamp":1791247162000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-parasitology\/volume-110\/issue-3\/23-100\/A-Simple-Quick-and-Economical-Method-for-in-Vitro-Cultivation\/10.1645\/23-100.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,30]]},"references-count":25,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,6,1]]}},"URL":"https:\/\/doi.org\/10.1645\/23-100","relation":{},"ISSN":["0022-3395","1937-2345"],"issn-type":[{"value":"0022-3395","type":"print"},{"value":"1937-2345","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,30]]}}}