{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:54:45Z","timestamp":1791248085233,"version":"4.1.0"},"reference-count":44,"publisher":"American Society of Parasitologists","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology"],"published-print":{"date-parts":[[2024,9,9]]},"abstract":"<jats:p>Chlorocresol has antibacterial and antifungal properties, yet its effectiveness in eradicating Acanthamoeba spp. remains unexplored. Acanthamoeba species trophozoites are usually sensitive to biocides, whereas cysts tend to be more resistant. This study aimed to evaluate the cysticidal activity of chlorocresol against Acanthamoeba polyphaga. Chlorocresol concentrations of 0.02, 0.04, and 0.08% were prepared and A. polyphaga cysts were incubated at room temperature (28\u201337 C) for 1, 24, 48, and 72 hr at each concentration. Cyst viability was evaluated using trypan blue staining and the percentage of nonviable cysts was calculated. For qualification assays, treated cysts were cultured on nonnutrient agar medium coated with Escherichia coli, incubated at 30 C, observed under a stereomicroscope for 30 days, and inoculated into peptone\u2013yeast extract\u2013glucose medium at 30 C for 72 hr. The results revealed that the A. polyphaga cysts were susceptible to 0.02, 0.04, and 0.08% chlorocresol. Chlorocresol made a significant difference in viability (P &lt; 0.001) compared with the nontreated control for the same incubation time. This is the first study to examine the efficacy of chlorocresol against A. polyphaga cysts and it was highly effective. Chlorocresol could thus serve as an alternative chemical disinfectant for the eradication of A. polyphaga cysts as well as a prophylactic against transmission of other pathogenic microorganisms for which Acanthamoeba species can act as a carrier.<\/jats:p>","DOI":"10.1645\/24-15","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T05:29:59Z","timestamp":1725859799000},"page":"423-427","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Cysticidal Activity of Chlorocresol Against Acanthamoeba polyphaga"],"prefix":"10.1645","volume":"110","author":[{"given":"Orlanath","family":"Phendinvay","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen Province, Mitraparp Road, Thailand 40002."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaksaporn","family":"Thongseesuksai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen Province, Mitraparp Road, Thailand 40002."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thidarut","family":"Boonmars","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen Province, Mitraparp Road, Thailand 40002."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2207-2051","authenticated-orcid":false,"given":"Porntip","family":"Laummaunwai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen Province, Mitraparp Road, Thailand 40002."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1220","reference":[{"key":"23","doi-asserted-by":"crossref","unstructured":"F. Marciano-Cabral  and G. Cabral  \"Clinical Microbiology Reviews.\"  16, \"Acanthamoeba spp. as agents of disease in humans.\" 273 (2003)","DOI":"10.1128\/CMR.16.2.273-307.2003"},{"key":"1","doi-asserted-by":"publisher","unstructured":"A. Anwar, N. A. Khan, R. Siddiqui,  \"Parasites & Vectors.\"  11, \"Combating Acanthamoeba spp. cysts: What are the options?\" 26 (2018)","DOI":"10.1186\/s13071-017-2572-z"},{"key":"2","doi-asserted-by":"crossref","unstructured":"O. I. Aremu, O. H. Badru, O. A. Oyemade,  \"African Research Review.\"  3, \"Comparative effectiveness of certain antimicrobial agents in semi solid preparations.\" 241 (2009)","DOI":"10.4314\/afrrev.v3i4.47560"},{"key":"3","doi-asserted-by":"crossref","unstructured":"F. Arnalich-Montiel, Lumbreras-Fern\u00e1ndez B., C. M. Mart\u00ednnavarro, et al.  \"Journal of Clinical Microbiology.\"  52, \"Influence of Acanthamoeba genotype on clinical course and outcomes for patients with Acanthamoeba keratitis in Spain.\" 1213 (2014)","DOI":"10.1128\/JCM.00031-14"},{"key":"4","doi-asserted-by":"crossref","unstructured":"A. M. Baig, J. Iqbal, N. A. Khan,  \"Antimicrobial Agents and Chemotherapy.\"  57, \"In vitro efficacies of clinically available drugs against growth and viability of an Acanthamoeba castellanii keratitis isolate belonging to the T4 genotype.\" 3561 (2013)","DOI":"10.1128\/AAC.00299-13"},{"key":"5","doi-asserted-by":"publisher","unstructured":"C. Balczun  and P. L. Scheid  \"Viruses.\"  9, \"Free-living amoebae as hosts for and vectors of intracellular microorganisms with public health significance.\" 65 (2017)","DOI":"10.3390\/v9040065"},{"key":"6","unstructured":"\"Opinion on the application for approval of the active substance: Chlorocresol.\"  12 (2016)"},{"key":"7","doi-asserted-by":"crossref","unstructured":"P. J. Brofman, K. A. Knostman, S. P. Dibartola,  \"Journal of Veterinary Internal Medicine.\"  17, \"Granulomatous amebic meningoencephalitis causing the syndrome of inappropriate secretion of antidiuretic hormone in a dog.\" 230 (2003)","DOI":"10.1111\/j.1939-1676.2003.tb02439.x"},{"key":"8","doi-asserted-by":"crossref","unstructured":"L. C. Carrijo-Carvalho, Sant'ana V. P., A. S. Foronda, et al.  \"Survey of Ophthalmology.\"  62, \"Therapeutic agents and biocides for ocular infections by free-living amoebae of Acanthamoeba genus.\" 203 (2017)","DOI":"10.1016\/j.survophthal.2016.10.009"},{"key":"9","doi-asserted-by":"publisher","unstructured":"D. Corsaro,  \"Parasitology Research.\"  120, \"Correction to: Update on Acanthamoeba phylogeny.\" 1927 (2021)","DOI":"10.1007\/s00436-020-06843-9"},{"key":"10","doi-asserted-by":"crossref","unstructured":"D. Corsaro, M. K\u00f6hsler, D. F. M. Montalbano, et al.  \"Parasitology Research.\"  116, \"Update on Acanthamoeba jacobsi genotype T15, including full-length 18S rDNA molecular phylogeny.\" 1273 (2017)","DOI":"10.1007\/s00436-017-5406-1"},{"key":"11","doi-asserted-by":"crossref","unstructured":"C. Coulon, A. Collignon, G. Mcdonnell, et al.  \"Journal of Clinical Microbiology.\"  48, \"Resistance of Acanthamoeba cysts to disinfection treatments used in health care settings.\" 2689 (2010)","DOI":"10.1128\/JCM.00309-10"},{"key":"12","doi-asserted-by":"crossref","unstructured":"J. P. Dubey, J. E. Benson, K. T. Blakeley, et al.  \"Veterinary Parasitology.\"  128, \"Disseminated Acanthamoeba sp. infection in a dog.\" 183 (2005)","DOI":"10.1016\/j.vetpar.2004.11.022"},{"key":"13","doi-asserted-by":"crossref","unstructured":"I. Dykov\u00e1, J. Lom, Schroeder-Diedrich J. M., et al.  \"Journal of Parasitology.\"  85, \"Acanthamoeba strains isolated from organs of freshwater fishes.\" 1106 (1999)","DOI":"10.2307\/3285675"},{"key":"14","unstructured":"\"Chlorocresol (4-chloro-3-meth-ylphenol): Summary Report Committee for veterinary medicinal products: EMEA\/MRL\/074\/96-FINAL.\"  3 (1996)"},{"key":"15","doi-asserted-by":"crossref","unstructured":"P. A. Fuerst, G. C. Booton, M. Crary,  \"Journal of Eukaryotic Microbiology.\"  62, \"Phylogenetic analysis and the evolution of the 18S rRNA gene typing system of Acanthamoeba.\" 69 (2015)","DOI":"10.1111\/jeu.12186"},{"key":"16","doi-asserted-by":"crossref","unstructured":"Y. Hirukawa, H. Nakato, S. Izumi, et al.  \"Biochimica et Biophysica Acta.\"  1398, \"Structure and expression of a cyst specific protein of Acanthamoeba castellanii.\" 47 (1988)","DOI":"10.1016\/S0167-4781(98)00026-8"},{"key":"17","unstructured":"I. Ithoi, R. Mahmud, Abdul Basher M. H., et al.  \"Tropical Biomedicine.\"  30, \"Acanthamoeba genotype T4 detected in naturally-infected feline corneas found to be in homology with those causing human keratitis.\" 131 (2013)"},{"key":"18","doi-asserted-by":"crossref","unstructured":"M. D. Johnston, G. W. Hanlon, S. P. Denyer, et al.  \"Journal of Applied Microbiology.\"  94, \"Membrane damage to bacteria caused by single and combined biocides.\" 1015 (2003)","DOI":"10.1046\/j.1365-2672.2003.01923.x"},{"key":"19","doi-asserted-by":"crossref","unstructured":"N. A. Khan,  \"Microbial Pathogenesis.\"  34, \"Pathogenesis of Acanthamoeba infections.\" 277 (2003)","DOI":"10.1016\/S0882-4010(03)00061-5"},{"key":"20","doi-asserted-by":"crossref","unstructured":"L. Lim, D. J. Coster, P. R. Badenoch,  \"Clinical and Experimental Ophthalmology.\"  28, \"Antimicrobial susceptibility of 19 Australian corneal isolates of Acanthamoeba.\" 119 (2000)","DOI":"10.1046\/j.1442-9071.2000.00275.x"},{"key":"21","doi-asserted-by":"crossref","unstructured":"J. Lorenzo-Morales, N. A. Khan, J. Walochnik,  \"Parasite.\"  22, \"An update on Acanthamoeba keratitis: Diagnosis, pathogenesis and treatment.\" 1 (2015)","DOI":"10.1051\/parasite\/2015010"},{"key":"22","doi-asserted-by":"crossref","unstructured":"A. H. Maghsood, J. Sissons, M. Rezaian, et al.  \"Journal of Medical Microbiology.\"  54, \"Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates.\" 755 (2005)","DOI":"10.1099\/jmm.0.45970-0"},{"key":"24","doi-asserted-by":"crossref","unstructured":"C. M. Mart\u00edn-Navarro, L\u00f3pez-Arencibia A., Arnalich-Montiel F., et al.  \"Graefe's Archive for Clinical and Experimental Ophthalmology.\"  251, \"Evaluation of the in vitro activity of commercially available moxifloxacin and voriconazole eye-drops against clinical strains of Acanthamoeba.\" 2111 (2013)","DOI":"10.1007\/s00417-013-2371-y"},{"key":"25","doi-asserted-by":"crossref","unstructured":"G. Mcdonnell  and A. D. Russell  \"Clinical Microbiology Reviews.\"  12, \"Antiseptics and disinfectants: Activity, action, and resistance.\" 147 (1999)","DOI":"10.1128\/CMR.12.1.147"},{"key":"26","unstructured":"\"4-Chloro-3-methylphenol.\"  (2021)"},{"key":"27","unstructured":"R. J. Neff  and R. H. Neff  \"Symposia of the Society for Experimental Biology.\"  23, \"The biochemistry of amoebic encystment.\" 51 (1969)"},{"key":"28","doi-asserted-by":"crossref","unstructured":"C. Palmas, D. Wakelin, F. Gabriele,  \"Parasitology.\"  89, \"Transfer of immunity against Hymenolepis nana in mice with lymphoid cells or serum from infected donors.\" 287 (1984)","DOI":"10.1017\/S0031182000001311"},{"key":"29","doi-asserted-by":"crossref","unstructured":"Z. A. Polat, A. Vural, F. Ozan, et al.  \"Journal of Ocular Pharmacology and Therapeutics.\"  24, \"In vitro evaluation of the amoebicidal activity of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells.\" 8 (2008)","DOI":"10.1089\/jop.2007.0035"},{"key":"30","doi-asserted-by":"crossref","unstructured":"M. Richter, F. Matheis, E. G\u00f6nczi, et al.  \"Veterinary Ophthalmology.\"  13, \"Parachlamydia acanthamoebae in domestic cats with and without corneal disease.\" 235 (2010)","DOI":"10.1111\/j.1463-5224.2010.00789.x"},{"key":"31","doi-asserted-by":"crossref","unstructured":"J. M. Schroeder, G. C. Booton, J. Hay, et al.  \"Journal of Clinical Microbiology.\"  39, \"Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge.\" 1903 (2001)","DOI":"10.1128\/JCM.39.5.1903-1911.2001"},{"key":"33","doi-asserted-by":"crossref","unstructured":"M. J. M. Sesma  and L. Z. Ramos  \"Journal of Parasitology.\"  75, \"Isolation of free-living amoebas from the intestinal contents of reptiles.\" 322 (1989)","DOI":"10.2307\/3282785"},{"key":"34","doi-asserted-by":"crossref","unstructured":"I. Sifaoui, Reyes-Batlle M., L\u00f3pez-Arencibia A., et al.  \"Experimental Parasitology.\"  183, \"Evaluation of the anti-Acanthamoeba activity of two commercial eye drops commonly used to lower eye pressure.\" 117 (2017)","DOI":"10.1016\/j.exppara.2017.07.012"},{"key":"35","doi-asserted-by":"crossref","unstructured":"E. Straberg  and A. Daugschies  \"Parasitology Research.\"  101, \"Control of piglet coccidiosis by chemical disinfection with a cresol-based product (Neopredisan 135-1).\" 599 (2007)","DOI":"10.1007\/s00436-007-0521-z"},{"key":"36","doi-asserted-by":"crossref","unstructured":"T. Thammaratana, P. Laummaunwai, T. Boonmars,  \"Parasitology Research.\"  115, \"Isolation and identification of Acanthamoeba species from natural water sources in the northeastern part of Thailand.\" 1705 (2016)","DOI":"10.1007\/s00436-016-4911-y"},{"key":"37","doi-asserted-by":"crossref","unstructured":"G. Tomlinson  and E. A. Jones  \"Biochimica et Biophysica Acta.\"  63, \"Isolation of cellulose from the cyst wall of a soil amoeba.\" 194 (1962)","DOI":"10.1016\/0006-3002(62)90353-0"},{"key":"38","doi-asserted-by":"crossref","unstructured":"N. A. Turner, A. D. Russell, J. R. Furr, et al.  \"Journal of Applied Microbiology.\"  96, \"Resistance, biguanide sorption and biguanide-induced pentose leakage during encystment of Acanthamoeba castellanii.\" 1287 (2004)","DOI":"10.1111\/j.1365-2672.2004.02260.x"},{"key":"39","doi-asserted-by":"crossref","unstructured":"M. Valladares, Reyes-Batlle M., Mart\u00edn-Navarro C. M., et al.  \"Archives of Microbiology.\"  197, \"Molecular characterization of Acanthamoeba strains isolated from domestic dogs in Tenerife, Canary Islands, Spain.\" 639 (2015)","DOI":"10.1007\/s00203-015-1096-1"},{"key":"40","doi-asserted-by":"crossref","unstructured":"M. Valladares, Reyes-Batlle M., Mora-Peces I., et al.  \"Veterinary Parasitology.\"  205, \"A multisystemic Acanthamoeba infection in a dog in Tenerife, Canary Islands, Spain.\" 707 (2014)","DOI":"10.1016\/j.vetpar.2014.08.004"},{"key":"41","doi-asserted-by":"crossref","unstructured":"G. S. Visvesvara,  \"Journal of Neuroinfectious Diseases.\"  1, \"Free-living amebae as opportunistic agents of human disease.\" 1 (2010)","DOI":"10.4303\/jnp\/N100802"},{"key":"42","doi-asserted-by":"crossref","unstructured":"J. Walochnik, A. Hassl, K. Simon, et al.  \"Parasitology Research.\"  85, \"Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basiliscus plumifrons (Sauria: Iguanidae).\" 601 (1999)","DOI":"10.1007\/s004360050602"},{"key":"43","doi-asserted-by":"publisher","unstructured":"Y. Wang, L. Jiang, Y. Zhao, et al.  \"Frontiers in Microbiology.\"  14, \"Biological characteristics and pathogenicity of Acanthamoeba.\" 1147077 (2023)","DOI":"10.3389\/fmicb.2023.1147077"},{"key":"44","doi-asserted-by":"crossref","unstructured":"S. Zhang, C. Angel, X. Gu, et al.  \"Parasitology Research.\"  119, \"Efficacy of a chlorocresol-based disinfectant product on Toxocara canis eggs.\" 3369 (2020)","DOI":"10.1007\/s00436-020-06769-2"},{"key":"32","doi-asserted-by":"publisher","unstructured":"F. L. Schuster  and G. S. Visvesvara  \"International Journal for Parasitology.\"  34, \"Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals.\" 1001 (2004)","DOI":"10.1016\/j.ijpara.2004.06.004"}],"container-title":["Journal of Parasitology"],"original-title":[],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:16:42Z","timestamp":1791245802000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-parasitology\/volume-110\/issue-5\/24-15\/Evaluation-of-the-Cysticidal-Activity-of-Chlorocresol-Against-Acanthamoeba-polyphaga\/10.1645\/24-15.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,9]]},"references-count":44,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,9,1]]}},"URL":"https:\/\/doi.org\/10.1645\/24-15","relation":{},"ISSN":["0022-3395","1937-2345"],"issn-type":[{"value":"0022-3395","type":"print"},{"value":"1937-2345","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,9]]}}}