{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:50:59Z","timestamp":1791247859318,"version":"4.1.0"},"reference-count":33,"publisher":"American Society of Parasitologists","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology"],"published-print":{"date-parts":[[2024,12,20]]},"abstract":"<jats:p>Accurate detection of schistosome infections in snails is vital for epidemiologic and laboratory studies. Traditional microscopy methods to detect schistosomes in snails are hindered by long prepatent periods and snail survivorship, leading to inaccurate assessment of infections. A rapid, multiplexed PCR assay targeting Biomphalaria sudanica or Biomphalaria glabrata (internal control) and Schistosoma mansoni DNA is described. The method takes less than 90 min starting from extracted snail DNA and is successful at amplifying schistosome DNA in snail tissue as soon as 30 min following exposure. Accurate measures of schistosome infection success in snails (compatibility) are possible by 4\u20137 days postexposure.<\/jats:p>","DOI":"10.1645\/24-44","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T01:23:36Z","timestamp":1735262616000},"page":"684-689","source":"Crossref","is-referenced-by-count":4,"title":["A Rapid Diagnostic PCR Assay for the Detection of Schistosoma mansoni in Their Snail Vectors"],"prefix":"10.1645","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4898-462X","authenticated-orcid":false,"given":"Tom","family":"Pennance","sequence":"first","affiliation":[{"name":"College of Osteopathic Medicine of the Pacific-Northwest, Western University of Health Sciences, Lebanon, Oregon 97355."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Lam","sequence":"additional","affiliation":[{"name":"College of Osteopathic Medicine of the Pacific-Northwest, Western University of Health Sciences, Lebanon, Oregon 97355."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel","family":"Bigot","sequence":"additional","affiliation":[{"name":"College of Osteopathic Medicine of the Pacific-Northwest, Western University of Health Sciences, Lebanon, Oregon 97355."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Trapp","sequence":"additional","affiliation":[{"name":"College of Osteopathic Medicine of the Pacific-Northwest, Western University of Health Sciences, Lebanon, Oregon 97355."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannie M.","family":"Spaan","sequence":"additional","affiliation":[{"name":"College of Osteopathic Medicine of the Pacific-Northwest, Western University of Health Sciences, Lebanon, Oregon 97355."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Ogara","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute (KEMRI), P.O. Box 1578-40100, Kisumu, Kenya."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrick","family":"Rawago","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute (KEMRI), P.O. Box 1578-40100, Kisumu, Kenya."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kennedy","family":"Andiego","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute (KEMRI), P.O. Box 1578-40100, Kisumu, Kenya."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boaz","family":"Mulonga","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute (KEMRI), P.O. Box 1578-40100, Kisumu, Kenya."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meredith","family":"Odhiambo","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute (KEMRI), P.O. Box 1578-40100, Kisumu, Kenya."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin W.","family":"Mutuku","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Research and Development, Kenya Medical Research Institute (KEMRI), P.O. Box 54840-00200, Nairobi, Kenya."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Loker","sequence":"additional","affiliation":[{"name":"Parasitology Division, Museum of Southwestern Biology, University of New Mexico, Albuquerque 87131."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurice R.","family":"Odiere","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Kenya Medical Research Institute (KEMRI), P.O. Box 1578-40100, Kisumu, Kenya."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L.","family":"Steinauer","sequence":"additional","affiliation":[{"name":"College of Osteopathic Medicine of the Pacific-Northwest, Western University of Health Sciences, Lebanon, Oregon 97355."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1220","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"I. Abbasi, C. H. King, E. M. 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Wearing, et al.  \"eLife.\"  8, \"Antagonism between parasites within snail hosts impacts the transmission of human schistosomiasis.\" e50095 (2019)","DOI":"10.7554\/eLife.50095"}],"container-title":["Journal of Parasitology"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:53:37Z","timestamp":1791244417000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-parasitology\/volume-110\/issue-6\/24-44\/A-Rapid-Diagnostic-PCR-Assay-for-the-Detection-of-Schistosoma\/10.1645\/24-44.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,20]]},"references-count":33,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,11,1]]}},"URL":"https:\/\/doi.org\/10.1645\/24-44","relation":{},"ISSN":["0022-3395","1937-2345"],"issn-type":[{"value":"0022-3395","type":"print"},{"value":"1937-2345","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,20]]}}}