{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:00:47Z","timestamp":1791234047680,"version":"4.1.0"},"reference-count":15,"publisher":"American Society of Parasitologists","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology"],"published-print":{"date-parts":[[2026,7,30]]},"abstract":"<jats:p>Most species of endotherms and some ectotherms harbor muscle cysts (sarcocysts) of 1 or more species of apicomplexan parasites in the genus Sarcocystis. We report a novel species discovered in the muscles of a Canada lynx (Lynx canadensis). We found microscopic sarcocysts in limb muscles of 1 of 3 endangered Canada lynx from Minnesota. The sarcocyst wall was relatively thin (&lt;2 \u00b5m) and had short, stubby protrusions with apical thickening. We characterized these further by sequencing the 18S, 28S, and ITS1 portions of nuclear ribosomal RNA genes. The sequences of each marker perfectly matched those of an undescribed species of Sarcocystis previously identified in the brains of river otters (Lontra canadensis) in Alberta, Canada. We propose Sarcocystis canadensis n. sp. for the parasite.<\/jats:p>","DOI":"10.1645\/26-37","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T03:07:24Z","timestamp":1785467244000},"page":"436-442","source":"Crossref","is-referenced-by-count":0,"title":["Sarcocystis canadensis n. sp. (Protozoa: Sarcocystidae) from Limb Muscles of the Canada Lynx (Lynx canadensis)"],"prefix":"10.1645","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4455-2870","authenticated-orcid":false,"given":"J. P.","family":"Dubey","sequence":"first","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland 20705-2350."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditya","family":"Gupta","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland 20705-2350."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin","family":"Humpal","sequence":"additional","affiliation":[{"name":"Minnesota Department of Natural Resources, Forest Wildlife Populations and Research Group, 1201 E Highway 2, Grand Rapids, Minnesota 55744."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Carstensen","sequence":"additional","affiliation":[{"name":"Minnesota Department of Natural Resources, Wildlife Health Program, 5463 West Broadway, Forest Lake, Minnesota 55025."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. M.","family":"Rosenthal","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland 20705-2350."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1220","reference":[{"key":"10","doi-asserted-by":"publisher","unstructured":"L. Kutkien\u0117, P. Prakas, A. Sruoga, et al.  \"Parasitology Research.\"  107, \"The mallard duck (Anas platyrhynchos) as intermediate host for Sarcocystis wobeseri sp. nov. from the barnacle goose (Branta leucopsis).\" 879 (2010)","DOI":"10.1007\/s00436-010-1945-4"},{"key":"11","doi-asserted-by":"publisher","unstructured":"G. Mor\u00e9, S. Schares, A. Maksimov, et al.  \"Veterinary Parasitology.\"  197, \"Development of a multiplex real time PCR to differentiate Sarcocystis spp. affecting cattle.\" 85 (2013)","DOI":"10.1016\/j.vetpar.2013.04.024"},{"key":"1","doi-asserted-by":"publisher","unstructured":"J. Bell,  \"Biotechniques.\"  44, \"A simple way to treat PCR products prior to sequencing using ExoSAP-IT.\" 834 (2008)","DOI":"10.2144\/000112890"},{"key":"3","doi-asserted-by":"publisher","unstructured":"J. P. Dubey, A. Gupta, M. V. Reichard, et al.  \"Journal of Parasitology.\"  112, \"Confirmation of Sarcocystis infections in tongues of Florida panthers (Puma concolor coryi).\" 305 (2026)","DOI":"10.1645\/26-14"},{"key":"4","doi-asserted-by":"publisher","unstructured":"J. P. Dubey, A. Gupta, B. M. Rosenthal, et al.  \"Veterinary Parasitology: Regional Studies and Reports.\"  63, \"Sarcocystis infections in bobcats (Lynx rufus) from Minnesota, USA.\" 101319 (2025)","DOI":"10.1016\/j.vprsr.2025.101319"},{"key":"5","doi-asserted-by":"publisher","unstructured":"J. P. Dubey, D. S. Lindsay, D. Fritz, et al.  \"Journal of Parasitology.\"  87, \"Structure of Sarcocystis neurona sarcocysts.\" 1323 (2001)","DOI":"10.1645\/0022-3395(2001)087[1323:SOSNS]2.0.CO;2"},{"key":"7","doi-asserted-by":"publisher","unstructured":"T. W. Forest, Abou-Madi N., B. A. Summers, et al.  \"Journal of Zoo and Wildlife Medicine.\"  31, \"Sarcocystis neurona\u2013like encephalitis in a Canada lynx (Felis lynx canadensis).\" 383 (2000)","DOI":"10.1638\/1042-7260(2000)031[0383:SNLEIA]2.0.CO;2"},{"key":"8","doi-asserted-by":"publisher","unstructured":"A. Gupta, C. Humpal, M. Carstensen, et al.  \"Veterinary Parasitology: Regional Studies and Reports.\"  67, \"First report of Sarcocystis bovifelis\u2013like sporocysts in the intestine of the Canadian lynx (Lynx canadensis) in USA.\" 101397 (2026)","DOI":"10.1016\/j.vprsr.2025.101397"},{"key":"9","doi-asserted-by":"publisher","unstructured":"S. Kumar, G. Stecher, M. Suleski, et al.  \"Molecular Biology and Evolution.\"  41, \"MEGA12: Molecular evolutionary genetic analysis version 12 for adaptive and green computing.\" msae263 (2024)","DOI":"10.1093\/molbev\/msae263"},{"key":"12","doi-asserted-by":"publisher","unstructured":"N. B. Mugridge, D. A. Morrison, A. R. Heckeroth, et al.  \"International Journal for Parasitology.\"  29, \"Phylogenetic analysis based on full-length large subunit ribosomal RNA gene sequence comparison reveals that Neospora caninum is more closely related to Hammondia heydorni than to Toxoplasma gondii.\" 1545 (1999)","DOI":"10.1016\/s0020-7519(99)00150-2"},{"key":"15","doi-asserted-by":"publisher","unstructured":"K. M. Shanebeck, Hern\u00e1ndez-Ortiz A., E. J. Jenkins, et al.  \"International Journal for Parasitology: Parasites and Wildlife.\"  27, \"Detection of Toxoplasma gondii (types I, II, III and 12) and Sarcocystis spp. in the brains of river otter (Lontra canadensis) from Alberta, Canada.\" 101069 (2025)","DOI":"10.1016\/j.ijppaw.2025.101069"},{"key":"14","doi-asserted-by":"publisher","unstructured":"I. Sela, H. Ashkenazy, K. Katoh, et al.  \"Nucleic Acids Research.\"  43, \"GUIDANCE2: Accurate detection of unreliable alignment regions accounting for the uncertainty of multiple parameters.\" W7 (2015)","DOI":"10.1093\/nar\/gkv318"},{"key":"2","doi-asserted-by":"publisher","unstructured":"J. P. Dubey, Calero-Bernal R., B. M. Rosenthal, et al.  \"Sarcocystosis of Animals and Humans.\"  501 (2016)","DOI":"10.1201\/b19184"},{"key":"13","doi-asserted-by":"publisher","unstructured":"L. T. Nguyen, H. A. Schmidt, Von Haeseler A., et al.  \"Molecular Biology and Evolution.\"  32, \"IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies.\" 268 (2015)","DOI":"10.1093\/molbev\/msu300"},{"key":"6","doi-asserted-by":"publisher","unstructured":"J. P. Dubey, J. E. Sykes, G. D. Shelton, et al.  \"Journal of Eukaryotic Microbiology.\"  62, \"Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) associated with severe myositis and hepatitis in the domestic dog (Canis familiaris).\" 307 (2015)","DOI":"10.1111\/jeu.12182"}],"container-title":["Journal of Parasitology"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:36:23Z","timestamp":1791232583000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-parasitology\/volume-112\/issue-4\/26-37\/Sarcocystis-canadensis-n-sp-Protozoa--Sarcocystidae-from-Limb-Muscles\/10.1645\/26-37.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":15,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,7,1]]}},"URL":"https:\/\/doi.org\/10.1645\/26-37","relation":{},"ISSN":["0022-3395","1937-2345"],"issn-type":[{"value":"0022-3395","type":"print"},{"value":"1937-2345","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]}}}