{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T06:31:08Z","timestamp":1793428268200,"version":"4.5.0"},"reference-count":23,"publisher":"Radiation Research Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2023,7,14]]},"abstract":"<jats:p>Connexin26 (Cx26) plays an important role in ionizing radiation-induced damage, and CC chemokine ligand 27 (CCL27) regulates the skin immune response. However, the relationship between Cx26 and CCL27 in radiation-induced skin damage is unclear. After X-ray irradiation, clonogenic survival and micronucleus formation were assessed in immortalized human keratinocytes (HaCaT). Proteins in the mitogen activated protein kinase (MAPK) signaling pathway and CCL27-related proteins were detected by immunoblotting. HaCaTCx26\u2013\/\u2013 cells were constructed to verify the effects of Cx26 on CCL27 secretion. A mouse model was established to examine the expression of CCL27 and skin inflammation in vivo. The degree of skin injury induced by 6 MV of X rays was closely related to CCL27. The phosphorylation of ERK, p38 and NF-\u03baB was significantly increased in irradiated cells. The secretion of CCL27 was significantly decreased in HaCaT wild-type cells relative to HaCaTCx26\u2013\/\u2013 cells. Whereas cell survival fractions decreased, and the micronuclei formation rate increased as a function of increasing X-ray dose in HaCaT cells, the opposite trend occurred in HaCaTCx26\u2013\/\u2013 cells. Our findings show that Cx26 likely plays a role in the activation of the MAPK and NF-\u03baB\/COX-2 signaling pathways and regulates the secretion of CCL27 in keratinocytes after X-ray radiation-induced skin damage.<\/jats:p>","DOI":"10.1667\/rade-20-00085.1","type":"journal-article","created":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T14:11:12Z","timestamp":1689343872000},"page":"281-288","source":"Crossref","is-referenced-by-count":5,"title":["Connexin26 Modulates Radiation-Induced Skin Damage by Regulating Chemokine CCL27 through MAPK Signaling"],"prefix":"10.1667","volume":"200","author":[{"given":"Rui","family":"Tao","sequence":"first","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Mao","sequence":"additional","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minqiong","family":"Sun","sequence":"additional","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Cao","sequence":"additional","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni","family":"Chen","sequence":"additional","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengmin","family":"Xu","sequence":"additional","affiliation":[{"name":"Institutes of Physical Sciences and Information Technology, Anhui University, Hefei 230601, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Zhao","sequence":"additional","affiliation":[{"name":"Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"15","doi-asserted-by":"crossref","unstructured":"S Zhang, W Wang, Q Gu, et al.  \"Free Radic Biol Med.\"  69, \"Protein and miRNA profiling of radiation-induced skin injury in rats: the protective role of peroxiredoxin-6 against ionizing radiation.\" 96 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suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes.\" 1403 (2004)","DOI":"10.1016\/j.jaci.2004.08.041"}],"container-title":["Radiation 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