{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:34:38Z","timestamp":1793439278511,"version":"4.5.0"},"reference-count":48,"publisher":"Radiation Research Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2021,6,9]]},"abstract":"<jats:p>Severe radiation-induced skin injury is a complication of tumor radiotherapy and nuclear accidents. Cell therapy is a potential treatment for radiation-induced skin injury. The stromal vascular fraction (SVF) is a newer material in stem cell therapy that is made up of stem cells harvested from adipose tissue, which has been shown to promote the healing of refractory wounds of different causes. In this study, SVF was isolated from patients with radiation-induced skin injury. Adipose-derived stem cells (ADSCs) accounted for approximately 10% of the SVF by flow cytometry. Compared with the control group of rats, administration with SVF attenuated the skin injury induced by electron beam radiation. The effect of SVF on the human skin fibroblast microenvironment was determined by proteomic profiling of secreted proteins in SVF-co-cultured human skin fibroblast WS1 cells. Results revealed 293 upregulated and 1,481 downregulated proteins in the supernatant of SVF-co-cultured WS1 cells. WS1 co-culture with SVF induced secretion of multiple proteins including collagen and MMP-1. In the clinic, five patients with radiation-induced skin injury were recruited to receive SVF transfer-based therapy, either alone or combined with flap transplantation. Autogenous SVF was isolated and introduced into a multi-needle precision electronic injection device, which automatically and aseptically distributed the SVF to the exact layer of the wound in an accurate amount. After SVF transfer, wound healing clearly improved and pain was significantly relieved. The patients' skin showed satisfactory texture and shape with no further wound recurrence. Our findings suggest that transplantation of SVF could be an effective countermeasure against severe radiation-induced skin injury.<\/jats:p>","DOI":"10.1667\/rade-20-00156.1","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T23:35:37Z","timestamp":1629848137000},"page":"250-260","source":"Crossref","is-referenced-by-count":15,"title":["Transplantation of the Stromal Vascular Fraction (SVF) Mitigates Severe Radiation-Induced Skin Injury"],"prefix":"10.1667","volume":"196","author":[{"given":"Daojiang","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaijun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Mo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youyou","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"2","doi-asserted-by":"crossref","unstructured":"D Bernard, MD Desruet, M Wolf, et al.  \"Ann Endocrinol (Paris).\"  75, \"Radioiodine therapy in benign thyroid disorders. Evaluation of French nuclear medicine practices.\" 241 (2014)","DOI":"10.1016\/j.ando.2014.07.863"},{"key":"3","doi-asserted-by":"crossref","unstructured":"I. Tsushima,  \"J Environ Sci Health A Tox Hazard Subst Environ Eng.\"  52, \"Behavior of radioactive materials and safety stock of contaminated sludge.\" 158 (2017)","DOI":"10.1080\/10934529.2016.1240486"},{"key":"4","doi-asserted-by":"crossref","unstructured":"Y Wang, W Tu, Y Tang, et al.  \"Radiat Med Protec.\"  1, \"Prevention and treatment for radiation-induced skin injury during radiotherapy.\" 60 (2020)","DOI":"10.1016\/j.radmp.2020.02.004"},{"key":"5","doi-asserted-by":"crossref","unstructured":"RR Driskell, CAB Jahoda, C-M Chuong, et al.  \"Exp Dermatol.\"  23, \"Defining dermal adipose tissue.\" 629 (2014)","DOI":"10.1111\/exd.12450"},{"key":"6","doi-asserted-by":"crossref","unstructured":"CF Guerrero-Juarez  and MV Plikus  \"Nat Rev Endocrinol.\"  14, \"Emerging nonmetabolic functions of skin fat.\" 163 (2018)","DOI":"10.1038\/nrendo.2017.162"},{"key":"7","doi-asserted-by":"crossref","unstructured":"E Festa, J Fretz, R Berry, et al.  \"Cell.\"  146, \"Adipocyte lineage cells contribute to the skin stem cell niche to drive hair cycling.\" 761 (2011)","DOI":"10.1016\/j.cell.2011.07.019"},{"key":"8","doi-asserted-by":"crossref","unstructured":"BA Schmidt  and V Horsley  \"Development.\"  140, \"Intradermal adipocytes mediate fibroblast recruitment during skin wound healing.\" 1517 (2013)","DOI":"10.1242\/dev.087593"},{"key":"9","doi-asserted-by":"crossref","unstructured":"Y Xiao, W Mo, H Jia, et al.  \"J Dermatol Sci.\"  97, \"Ionizing radiation induces cutaneous lipid remolding and skin adipocytes confer protection against radiation-induced skin injury.\" 52 (2020)","DOI":"10.1016\/j.jdermsci.2020.01.009"},{"key":"10","doi-asserted-by":"crossref","unstructured":"S Akita, K Akino, A Hirano, et al.  \"Stem Cells Int.\"  2010, \"Noncultured autologous adipose-derived stem cells therapy for chronic radiation injury.\" 532704 (2010)","DOI":"10.4061\/2010\/532704"},{"key":"11","doi-asserted-by":"crossref","unstructured":"B Bertrand, J Eraud, M Velier, et al.  \"Burns.\"  S0305-4179, \"Supportive use of platelet-rich plasma and stromal vascular fraction for cell-assisted fat transfer of skin radiation-induced lesions in nude mice.\" 30328 (2020)","DOI":"10.1016\/j.burns.2020.04.020"},{"key":"13","doi-asserted-by":"crossref","unstructured":"CJ Iddins, SR Cohen, RE Goans, et al.  \"Health physics.\"  111, \"Case report: Industrial X-ray injury treated with non-cultured autologous adipose-derived stromal vascular fraction (SVF).\" 112 (2016)","DOI":"10.1097\/HP.0000000000000483"},{"key":"14","doi-asserted-by":"crossref","unstructured":"B Phulpin, P Gangloff, N Tran, et al.  \"Plast Reconstr Surg.\"  123, \"Rehabilitation of irradiated head and neck tissues by autologous fat transplantation.\" 1187 (2009)","DOI":"10.1097\/PRS.0b013e31819f2928"},{"key":"15","doi-asserted-by":"crossref","unstructured":"A Purkayastha, N Sharma, A Sarin, et al.  \"Asia Pac J Oncol Nurs.\"  6, \"Radiation fibrosis syndrome: the evergreen menace of radiation therapy.\" 238 (2019)","DOI":"10.4103\/apjon.apjon_71_18"},{"key":"16","doi-asserted-by":"crossref","unstructured":"G Rigotti, A Marchi, M Gali\u00e8, et al.  \"Plast Reconstr Surg.\"  119, \"Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells.\" 1409 (2007)","DOI":"10.1097\/01.prs.0000256047.47909.71"},{"key":"17","doi-asserted-by":"crossref","unstructured":"SM Sultan, CS Stern, ARJ Jr, et al.  \"Plast Reconstr Surg.\"  128, \"Human fat grafting alleviates radiation skin damage in a murine model.\" 363 (2011)","DOI":"10.1097\/PRS.0b013e31821e6e90"},{"key":"18","doi-asserted-by":"crossref","unstructured":"A Nguyen, J Guo, DA Banyard, et al.  \"J Plast Reconstr Aesthet Surg.\"  69, \"Stromal vascular fraction: A regenerative reality? Part 1: Current concepts and review of the literature.\" 170 (2016)","DOI":"10.1016\/j.bjps.2015.10.015"},{"key":"19","doi-asserted-by":"crossref","unstructured":"S Mehranfar, I Abdi Rad, E Mostafav, et al.  \"Artif Cells Nanomed Biotechnol.\"  47, \"The use of stromal vascular fraction (SVF), platelet-rich plasma (PRP) and stem cells in the treatment of osteoarthritis: an overview of clinical trials.\" 882 (2019)","DOI":"10.1080\/21691401.2019.1576710"},{"key":"20","doi-asserted-by":"crossref","unstructured":"I Andia, N Maffulli, N Burgos-Alonso,  \"Expert Opin Biol Ther.\"  19, \"Stromal vascular fraction technologies and clinical applications.\" 1289 (2019)","DOI":"10.1080\/14712598.2019.1671970"},{"key":"21","doi-asserted-by":"crossref","unstructured":"P Bora  and AS Majumdar  \"Stem Cell Res Ther.\"  8, \"Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation.\" 145 (2017)","DOI":"10.1186\/s13287-017-0598-y"},{"key":"22","doi-asserted-by":"crossref","unstructured":"N Gibbs, R Diamond, EO Sekyere, et al.  \"J Pain Res.\"  8, \"Management of knee osteoarthritis by combined stromal vascular fraction cell therapy, platelet-rich plasma, and musculoskeletal exercises: a case series.\" 799 (2015)","DOI":"10.2147\/JPR.S92090"},{"key":"23","doi-asserted-by":"crossref","unstructured":"YJ Koh, BI Koh, H Kim, et al.  \"Arterioscler Thromb Vasc Biol.\"  31, \"Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells.\" 1141 (2011)","DOI":"10.1161\/ATVBAHA.110.218206"},{"key":"24","doi-asserted-by":"crossref","unstructured":"J Guo, A Nguyen, DA Banyard, et al.  \"J Plast Reconstr Aesthet Surg.\"  69, \"Stromal vascular fraction: A regenerative reality? Part 2: Mechanisms of regenerative action.\" 180 (2016)","DOI":"10.1016\/j.bjps.2015.10.014"},{"key":"25","doi-asserted-by":"crossref","unstructured":"W Wang, J Luo, W Sheng, et al.  \"Int J Radiat Oncol Biol Phys.\"  95, \"Proteomic profiling of radiation-induced skin fibrosis in rats: Targeting the ubiquitin-proteasome system.\" 751 (2016)","DOI":"10.1016\/j.ijrobp.2016.01.021"},{"key":"26","doi-asserted-by":"crossref","unstructured":"J Xue, C Yu, W Sheng, et al.  \"J Invest Dermatol.\"  137, \"The Nrf2\/GCH1\/BH4 axis ameliorates radiation-induced skin injury by modulating the ROS cascade.\" 2059 (2017)","DOI":"10.1016\/j.jid.2017.05.019"},{"key":"27","doi-asserted-by":"crossref","unstructured":"G Chen, C Yu, Z Tang, et al.  \"Oncol Rep.\"  41, \"Metformin suppresses gastric cancer progression through calmodulin-like protein 3 secreted from tumor-associated fibroblasts.\" 405 (2019)","DOI":"10.3892\/or.2018.6783"},{"key":"28","doi-asserted-by":"crossref","unstructured":"YY Wang, DJ Yu, TL Zhao, et al.  \"Radiat Res.\"  191, \"Successful rescue of the victim exposed to a super high dose of iridium-192 during the Nanjing radiological accident in 2014.\" 527 (2019)","DOI":"10.1667\/RR15274.1"},{"key":"29","doi-asserted-by":"crossref","unstructured":"Y Xin, P Xu, X Wang, et al.  \"Stem Cell Res Ther.\"  12, \"Human foreskin-derived dermal stem\/progenitor cell-conditioned medium combined with hyaluronic acid promotes extracellular matrix regeneration in diabetic wounds.\" 49 (2021)","DOI":"10.1186\/s13287-020-02116-5"},{"key":"31","doi-asserted-by":"crossref","unstructured":"M. McQuestion,  \"Semin Oncol Nurs.\"  27, \"Evidence-based skin care management in radiation therapy: clinical update.\" e1 (2011)","DOI":"10.1016\/j.soncn.2011.02.009"},{"key":"32","doi-asserted-by":"crossref","unstructured":"L Shukla, Y Yuan, R Shayan, et al.  \"Front Pharmacol.\"  11, \"Fat therapeutics: the clinical capacity of adipose-derived stem cells and exosomes for human disease and tissue regeneration.\" 158 (2020)","DOI":"10.3389\/fphar.2020.00158"},{"key":"33","doi-asserted-by":"crossref","unstructured":"NH Nicolay, R Lopez Perez, J Debus, et al.  \"Cancer Lett.\"  366, \"Mesenchymal stem cells\u2013A new hope for radiotherapy-induced tissue damage?\" 133 (2015)","DOI":"10.1016\/j.canlet.2015.06.012"},{"key":"34","doi-asserted-by":"crossref","unstructured":"E Bey, P Duhamel, J-J Lataillade, et al.  \"Bull Acad Natl Med.\"  191, \"Treatment of radiation burns with surgery and cell therapy. A report of two cases. (Article in French).\" 971 (2007)","DOI":"10.1016\/S0001-4079(19)32994-2"},{"key":"36","doi-asserted-by":"crossref","unstructured":"M Portas, E Mansilla, H Drago, et al.  \"Radiat Prot Dosimetry.\"  171, \"Use of human cadaveric mesenchymal stem cells for cell therapy of a chronic radiation-induced skin lesion: A case report.\" 99 (2016)","DOI":"10.1093\/rpd\/ncw206"},{"key":"37","doi-asserted-by":"crossref","unstructured":"Z Liu, D Yu, J Xu, et al.  \"Biomed Pharmacother.\"  101, \"Human umbilical cord mesenchymal stem cells improve irradiation-induced skin ulcers healing of rat models.\" 729 (2018)","DOI":"10.1016\/j.biopha.2018.02.093"},{"key":"38","doi-asserted-by":"crossref","unstructured":"W-S Kim, B-S Park, J-H Sung,  \"Expert Opin Biol Ther.\"  9, \"The wound-healing and antioxidant effects of adipose-derived stem cells.\" 879 (2009)","DOI":"10.1517\/14712590903039684"},{"key":"39","doi-asserted-by":"crossref","unstructured":"E Nyberg, A Farris, A O'Sullivan, et al.  \"Tissue Eng Part A.\"  25, \"Comparison of stromal vascular fraction and passaged adipose-derived stromal\/stem cells as point-of-care agents for bone regeneration.\" 1459 (2019)","DOI":"10.1089\/ten.tea.2018.0341"},{"key":"40","doi-asserted-by":"crossref","unstructured":"SS Tan  and W Loh  \"Surgeon.\"  15, \"The utility of adipose-derived stem cells and stromal vascular fraction for oncologic soft tissue reconstruction: Is it safe? A matter for debate.\" 186 (2017)","DOI":"10.1016\/j.surge.2016.09.010"},{"key":"41","doi-asserted-by":"crossref","unstructured":"M Deptu\u0142a, A Brzezicka, A Skoniecka, et al.  \"Med Res Rev.\"  \"Adipose-derived stromal cells for nonhealing wounds: Emerging opportunities and challenges.\" (2021)","DOI":"10.1002\/med.21789"},{"key":"42","doi-asserted-by":"crossref","unstructured":"S Zhu, L Ye, S Bennett, et al.  \"J Cell Physiol.\"  236, \"Molecular structure and function of microfibrillar-associated proteins in skeletal and metabolic disorders and cancers.\" 41 (2021)","DOI":"10.1002\/jcp.29893"},{"key":"43","doi-asserted-by":"crossref","unstructured":"J-H Li, X-X Zhu, F-X Li, et al.  \"J Exp Clin Cancer Res.\"  38, \"MFAP5 facilitates the aggressiveness of intrahepatic Cholangiocarcinoma by activating the Notch1 signaling pathway.\" 476 (2019)","DOI":"10.1186\/s13046-019-1477-4"},{"key":"44","doi-asserted-by":"crossref","unstructured":"Y Wu, P Wu, Q Zhang, et al.  \"Cell Biosci.\"  9, \"MFAP5 promotes basal-like breast cancer progression by activating the EMT program.\" 24 (2019)","DOI":"10.1186\/s13578-019-0284-0"},{"key":"45","doi-asserted-by":"crossref","unstructured":"Z Chen, Y Lv, D Cao, et al.  \"Med Sci Monit.\"  26, \"Microfibril-associated protein 2 (MFAP2) potentiates invasion and migration of melanoma by EMT and Wnt\/beta-catenin pathway.\" e923808 (2020)","DOI":"10.12659\/MSM.923808"},{"key":"46","doi-asserted-by":"crossref","unstructured":"A Pardo  and M Selman  \"Int J Biochem Cell Biol.\"  37, \"MMP-1: the elder of the family.\" 283 (2005)","DOI":"10.1016\/j.biocel.2004.06.017"},{"key":"47","doi-asserted-by":"crossref","unstructured":"E Davison-Kotler, W Marshall, E Garcia-Gareta,  \"Bioengineering.\"  6, \"Sources of collagen for biomaterials in skin wound healing.\" 56 (2019)","DOI":"10.3390\/bioengineering6030056"},{"key":"48","doi-asserted-by":"crossref","unstructured":"S Lettmann, W Bloch, T Maass, et al.  \"PLoS One.\"  9, \"Col6a1 null mice as a model to study skin phenotypes in patients with collagen VI related myopathies: expression of classical and novel collagen VI variants during wound healing.\" e105686 (2014)","DOI":"10.1371\/journal.pone.0105686"},{"key":"35","doi-asserted-by":"crossref","unstructured":"E Bey, M Prat, P Duhamel, et al.  \"Wound Repair Regen.\"  18, \"Emerging therapy for improving wound repair of severe radiation burns using local bone marrow-derived stem cell administrations.\" 50 (2010)","DOI":"10.1111\/j.1524-475X.2009.00562.x"},{"key":"1","doi-asserted-by":"crossref","unstructured":"JL. Ryan,  \"J Invest Dermatol.\"  132, \"Ionizing radiation: the good, the bad, and the ugly.\" 985 (2012)","DOI":"10.1038\/jid.2011.411"},{"key":"12","doi-asserted-by":"crossref","unstructured":"TG Ebrahimian, F Pouzoulet, C Squiban, et al.  \"Arterioscler Thromb Vasc Biol.\"  29, \"Cell therapy based on adipose tissue-derived stromal cells promotes physiological and pathological wound healing.\" 503 (2009)","DOI":"10.1161\/ATVBAHA.108.178962"},{"key":"30","doi-asserted-by":"crossref","unstructured":"FN Bray, BJ Simmons, AH Wolfson, et al.  \"Dermatol Ther (Heidelb).\"  6, \"Acute and chronic cutaneous reactions to ionizing radiation therapy.\" 185 (2016)","DOI":"10.1007\/s13555-016-0120-y"}],"container-title":["Radiation Research"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:07:35Z","timestamp":1793437655000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/radiation-research\/volume-196\/issue-3\/RADE-20-00156.1\/Transplantation-of-the-Stromal-Vascular-Fraction-SVF-Mitigates-Severe-Radiation\/10.1667\/RADE-20-00156.1.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,9]]},"references-count":48,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,9,1]]}},"URL":"https:\/\/doi.org\/10.1667\/rade-20-00156.1","relation":{},"ISSN":["0033-7587","1938-5404"],"issn-type":[{"value":"0033-7587","type":"print"},{"value":"1938-5404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,9]]}}}