{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T08:34:18Z","timestamp":1793435658306,"version":"4.5.0"},"reference-count":34,"publisher":"Radiation Research Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2021,12,2]]},"abstract":"<jats:p>Hypoxia is associated with neoplastic tissue, protecting cancer cells from death by irradiation and chemotherapy. Identification of hypoxic volume of tumors could optimize patient selection for hypoxia-directed medical, immunological, and radiation therapies. Clostridium novyi-NT (CNV-NT) is an oncolytic bacterium derived from attenuated wild-type Clostridium novyi spores, which germinates exclusively in the anaerobic core of tumors with low-oxygen content. The hypothesis was that 64Cu-ATSM would localize to regions of hypoxia, and that greater hypoxic volume would result in greater germination of Clostridium novyi-NT (CNV-NT). Tumor-bearing companion dogs were recruited to a veterinary clinical trial. Dogs received a CT scan, 18F-FDG PET scan (74 MBq) and 64Cu-ATSM PET scan (74 MBq). Scan regions of interest were defined as the highest 20% of counts\/voxel for each PET scan, and regions with voxels overlapping between the two scans. Maximum standardized uptake value (MaxSUV) and threshold volume were calculated. Direct oximetry was performed in select tumors. Tumor types evaluated included nerve sheath tumor (10), apocrine carcinoma (1), melanoma (3) and oral sarcoma (6). MaxSUVATSM ranged from 0.3\u20136.6. Measured oxygen tension ranged from 0.05\u201389.9 mmHg. Inverse of MaxSUVATSM had a linear relationship with oxygen tension (R2 = 0.53, P = 0.0048). Hypoxia &lt;8 mmHg was associated with an SUVATSM &gt; 1.0. Hypoxic volume ranged from 0 to 100% of gross tumor volume (GTV) and MaxSUVATSM was positively correlated with hypoxic volume (R = 0.674; P = 0.0001), but not GTV (P = 0.182). Tumor hypoxic volume was heterogeneous in location and distribution. 64Cu-ATSM-avid regions were associated with differential CT attenuation. Hypoxic volume did not predict CNV-NT germination. 64Cu-ATSM PET scanning predicts hypoxia patterns within spontaneously occurring tumors of dogs as measured by direct oxymetry. Total tumor volume does not accurately predict degree or proportion of tumor hypoxia.<\/jats:p>","DOI":"10.1667\/rade-20-00186.1","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T14:38:23Z","timestamp":1638455903000},"page":"253-260","source":"Crossref","is-referenced-by-count":2,"title":["Paired 18F-Fluorodeoxyglucose (18F-FDG), and 64Cu-Copper(II)-diacetyl-bis(N(4)-methylthiosemicarbazone) (64Cu-ATSM) PET Scans in Dogs with Spontaneous Tumors and Evaluation for Hypoxia-Directed Therapy"],"prefix":"10.1667","volume":"197","author":[{"given":"Charles A.","family":"Maitz","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, University of Missouri \u2013 Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Tate","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, University of Missouri \u2013 Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Bechtel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, University of Missouri \u2013 Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joni","family":"Lunceford","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, University of Missouri \u2013 Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Henry","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, University of Missouri \u2013 Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Flesner","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, University of Missouri \u2013 Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Collins","sequence":"additional","affiliation":[{"name":"Biomed Valley Discoveries, Inc., Kansas City, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Varterasian","sequence":"additional","affiliation":[{"name":"Biomed Valley Discoveries, Inc., Kansas City, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Tung","sequence":"additional","affiliation":[{"name":"Biomed Valley Discoveries, Inc., Kansas City, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biomed Valley Discoveries, Inc., Kansas City, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh","family":"Saha","sequence":"additional","affiliation":[{"name":"Biomed Valley Discoveries, Inc., Kansas City, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey N.","family":"Bryan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Surgery, University of Missouri \u2013 Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"1","unstructured":"EJ Hall  and AJ Giaccia  \"Radiobiology for the Radiologist: Wolters Kluwer Health;.\"  (2018)"},{"key":"2","doi-asserted-by":"crossref","unstructured":"J Overgaard  and MR Horsman  \"Journal of Radiation Research.\"  57, \"The impact of hypoxia and its modification of the outcome of radiotherapy.\" i90 (2016)","DOI":"10.1093\/jrr\/rrw007"},{"key":"3","doi-asserted-by":"crossref","unstructured":"K Chao, W Bosch, S Mutic, et al.  \"International journal of radiation oncology, biology, physics.\"  49, \"A novel approach to overcome hypoxic tumor resistance: Cu-ATSM-guided intensity-modulated radiation therapy.\" 1171 (2001)","DOI":"10.1016\/S0360-3016(00)01433-4"},{"key":"4","doi-asserted-by":"crossref","unstructured":"J. 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Koch,  \"Radiation Research.\"  190, \"A Two-component assay for hypoxia incorporating long-term nitroreduction and short-term dna-damage allows differentiation of the three hypoxia sub-types\" 72 (2018)","DOI":"10.1667\/RR15029.1"},{"key":"34","doi-asserted-by":"crossref","unstructured":"CJ Koch, WT Jenkins, KW Jenkins, et al.  \"Tumor microenvironment and therapy.\"  1, \"Mechanisms of blood flow and hypoxia production in rat 9L-epigastric tumors.\" 1 (2013)","DOI":"10.2478\/tumor-2012-0001"}],"container-title":["Radiation Research"],"original-title":[],"link":[{"URL":"https:\/\/meridian.allenpress.com\/radiation-research\/article-pdf\/doi\/10.1667\/RADE-20-00186.1\/2971967\/10.1667_rade-20-00186.1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/meridian.allenpress.com\/radiation-research\/article-pdf\/doi\/10.1667\/RADE-20-00186.1\/2971967\/10.1667_rade-20-00186.1.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T08:10:07Z","timestamp":1793434207000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/radiation-research\/volume-197\/issue-3\/RADE-20-00186.1\/Paired-18F-Fluorodeoxyglucose-18F-FDG-and-64Cu-CopperII-diacetyl-bisN4\/10.1667\/RADE-20-00186.1.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,2]]},"references-count":34,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,3,1]]}},"URL":"https:\/\/doi.org\/10.1667\/rade-20-00186.1","relation":{},"ISSN":["0033-7587","1938-5404"],"issn-type":[{"value":"0033-7587","type":"print"},{"value":"1938-5404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,2]]}}}