{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:34:40Z","timestamp":1793439280872,"version":"4.5.0"},"reference-count":28,"publisher":"Radiation Research Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2021,6,9]]},"abstract":"<jats:p>Decreased neurogenesis after brain exposure to ionizing radiation is linked to neurocognitive impairments. Using transgenic mouse models, we previously showed that abrogation of radiation-induced senescence, or apoptosis, can partially rescue neurogenesis in the subventricular and hippocampus regions. Here, we evaluate whether the injection of recombinant epidermal growth factor (rEGF) or mesenchymal stromal cells (MSC) engineered to secrete EGF (MSC-EGF) can preserve neurogenesis. Using doublecortin (Dcx) expression and BrdU incorporation assays, we found that the injection of rEGF into the subventricular zone (SVZ) promotes neurogenesis, despite increasing apoptosis, in the brain of irradiated mice. The effect of rEGF was mostly localized, as Dcx expression was not induced in the hippocampus region and limited in the contralateral SVZ. Surprisingly, the injection of bone marrow-derived MSC alone, or secreting EGF, did not result in increased neurogenesis despite the fact that part of the MSC survived a few weeks after injection. Our results suggest that only a supraphysiological concentration of rEGF can promote neurogenesis, likely through a direct mitogenic effect.<\/jats:p>","DOI":"10.1667\/rade-21-00017.1","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T23:49:55Z","timestamp":1629848995000},"page":"315-322","source":"Crossref","is-referenced-by-count":4,"title":["The Intracerebroventricular Injection of Murine Mesenchymal Stromal Cells Engineered to Secrete Epidermal Growth Factor Does Not Prevent Loss of Neurogenesis in Irradiated Mice"],"prefix":"10.1667","volume":"196","author":[{"given":"Kerstin","family":"Killer","sequence":"first","affiliation":[{"name":"Centre de Recherche du CHU Ste-Justine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oanh","family":"Le","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHU Ste-Justine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Beaus\u00e9jour","sequence":"additional","affiliation":[{"name":"Centre de Recherche du CHU Ste-Justine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"KR Krull, TM Brinkman, C Li, et al.  \"J Clin Oncol.\"  31, \"Neurocognitive outcomes decades after treatment for childhood acute lymphoblastic leukemia: a report from the St Jude lifetime cohort study.\" 4407 (2013)","DOI":"10.1200\/JCO.2012.48.2315"},{"key":"3","doi-asserted-by":"crossref","unstructured":"E Tada, C Yang, GT Gobbel, et al.  \"Exp Neurol.\"  160, \"Long-term impairment of subependymal repopulation following damage by ionizing irradiation.\" 66 (1999)","DOI":"10.1006\/exnr.1999.7172"},{"key":"5","doi-asserted-by":"crossref","unstructured":"SF Sorrells, MF Paredes, A Cebrian-Silla, et al.  \"Nature.\"  555, \"Human hippocampal 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(2003)","DOI":"10.1126\/science.1088417"},{"key":"12","doi-asserted-by":"crossref","unstructured":"MJ Moravan, JA Olschowka, JP Williams, et al.  \"Radiat Res.\"  176, \"Cranial irradiation leads to acute and persistent neuroinflammation with delayed increases in T-cell infiltration and CD11c expression in C57BL\/6 mouse brain.\" 459 (2011)","DOI":"10.1667\/RR2587.1"}],"container-title":["Radiation Research"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:07:38Z","timestamp":1793437658000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/radiation-research\/volume-196\/issue-3\/RADE-21-00017.1\/The-Intracerebroventricular-Injection-of-Murine-Mesenchymal-Stromal-Cells-Engineered-to\/10.1667\/RADE-21-00017.1.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,9]]},"references-count":28,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,9,1]]}},"URL":"https:\/\/doi.org\/10.1667\/rade-21-00017.1","relation":{},"ISSN":["0033-7587","1938-5404"],"issn-type":[{"value":"0033-7587","type":"print"},{"value":"1938-5404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,9]]}}}