{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T17:06:24Z","timestamp":1793466384674,"version":"4.5.0"},"reference-count":50,"publisher":"Radiation Research Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2021,12,10]]},"abstract":"<jats:p>Microbiota can both negatively and positively impact radiation-induced bone loss. Our prior research showed that compared to mice with conventional gut microbiota (CM), mice with restricted gut microbiota (RM) reduced inflammatory tumor necrosis factor (TNF) in bone marrow, interleukin (IL)-17 in blood, and chemokine (C-C motif) ligand 20 (CCL20) in bone marrow under anti-IL-17 treatment. We showed that Muribaculum intestinale was more abundant in intestinal epithelial cells (IECs) from the small intestine of female RM mice and positively associated with augmented skeletal bone structure. Female C57BL\/6J pun RM mice, which were injected with anti-IL-17 antibody one day before exposure to 1.5 Gy 28Si ions of 850 MeV\/u, showed high trabecular numbers in tibiae at 6 weeks postirradiation. Irradiated CM mice were investigated for lower interferon-\u03b3 and IL-17 levels in the small intestine than RM mice. IL-17 blockage resulted in bacterial indicator phylotypes being different between both microbiota groups before and after irradiation. Analysis of the fecal bacteria were performed in relation to bone quality and body weight, showing reduced tibia cortical thickness in irradiated CM mice (\u201315%) vs. irradiated RM mice (\u20139.2%). Correlation analyses identified relationships among trabecular bone parameters (TRI-BV\/TV, Tb.N, Tb.Th, Tb.Sp) and Bacteroides massiliensis, Muribaculum sp. and Prevotella denticola. Turicibacter sp. was found directly correlated with trabecular separation in anti-IL-17 treated mice, whereas an unidentified Bacteroidetes correlated with trabecular thickness in anti-IL-17 neutralized and radiation-exposed mice. We demonstrated radiation-induced osteolytic damage to correlate with bacterial indicator phylotypes of the intestinal microbiota composition, and these relationships were determined from the previously discovered dose-dependent particle radiation effects on cell proliferation in bone tissue. New translational approaches were designed to investigate dynamic changes of gut microbiota in correlation with conditions of treatment and disease as well as mechanisms of systemic side-effects in radiotherapy.<\/jats:p>","DOI":"10.1667\/rade-21-00065.1","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T21:34:06Z","timestamp":1643664846000},"page":"184-192","source":"Crossref","is-referenced-by-count":1,"title":["Particle Radiation Side-Effects: Intestinal Microbiota Composition Shapes Interferon-\u03b3-Induced Osteo-Immunogenicity"],"prefix":"10.1667","volume":"197","author":[{"given":"Irene","family":"Maier","sequence":"first","affiliation":[{"name":"Department of Environmental Health Sciences, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Ruegger","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Deutschmann","sequence":"additional","affiliation":[{"name":"Department for Radiologic Technology, University of Applied Sciences Wiener Neustadt for Business and Engineering Ltd., Lower Austria, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas H.","family":"Helbich","sequence":"additional","affiliation":[{"name":"Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Pietschmann","sequence":"additional","affiliation":[{"name":"Institute of Pathophysiology and Allergy Research, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Schiestl","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Environmental Health Sciences, University of California, Los Angeles, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Borneman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Plant Pathology, University of California, Riverside, Riverside, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"18","doi-asserted-by":"crossref","unstructured":"ON Le, F Rodier, F Fontaine, et al.  \"Aging Cell.\"  9, \"Ionizing radiation-induced long-term expression of senescence markers in mice is independent of p53 and immune status.\" 398 (2010)","DOI":"10.1111\/j.1474-9726.2010.00567.x"},{"key":"30","doi-asserted-by":"crossref","unstructured":"YD Nam, HJ Kim, JG Seo, et al.  \"PLoS One.\"  8, \"Impact of pelvic radiotherapy on gut microbiota of gynecological cancer 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cervical cancer patients after radiation therapy.\" 968 (2013)","DOI":"10.1016\/j.ijrobp.2013.08.036"},{"key":"49","doi-asserted-by":"crossref","unstructured":"H Ikushima, K Osaki, S Furutani, et al.  \"Gynecol Oncol.\"  103, \"Pelvic bone complications following radiation therapy of gynecologic malignancies: clinical evaluation of radiation-induced pelvic insufficiency fractures.\" 1100 (2006)","DOI":"10.1016\/j.ygyno.2006.06.038"},{"key":"50","doi-asserted-by":"crossref","unstructured":"H Uezono, DJ Indelicato, RL Rotondo, et al.  \"Int J Radiat Oncol Biol Phys.\"  107, \"Treatment outcomes after proton therapy for ewing sarcoma of the pelvis.\" 974 (2020)","DOI":"10.1016\/j.ijrobp.2020.04.043"}],"container-title":["Radiation Research"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T08:07:04Z","timestamp":1793434024000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/radiation-research\/volume-197\/issue-2\/RADE-21-00065.1\/Particle-Radiation-Side-Effects--Intestinal-Microbiota-Composition-Shapes-Interferon\/10.1667\/RADE-21-00065.1.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,10]]},"references-count":50,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2,1]]}},"URL":"https:\/\/doi.org\/10.1667\/rade-21-00065.1","relation":{},"ISSN":["0033-7587","1938-5404"],"issn-type":[{"value":"0033-7587","type":"print"},{"value":"1938-5404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,10]]}}}