{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:32:41Z","timestamp":1793421161447,"version":"4.5.0"},"reference-count":53,"publisher":"Radiation Research Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2024,4,1]]},"abstract":"<jats:p>Radiation enteritis is a common complication of abdominal and pelvic radiotherapy. Several previous studies showed that fecal microbiota transplantation (FMT) could alleviate radiation enteritis. In this study, we investigated the efficacy of FMT in alleviating radiation enteritis and explored the mechanisms by multi-omics approaches. Briefly, C57BL\/6J mice were subjected to 9 Gy irradiation to the localized abdominal field, and randomized received FMT from healthy donor mice or saline. H&amp;E staining of harvested small intestine showed FMT decreased epithelial injury. Radiation-induced microbiota dysbiosis, characterized by a decrease in beneficial bacteria Lactobacillaceae and Lachnospiraceae, while these bacteria were restored by FMT. Fecal metabolomics analysis revealed that FMT modulated metabolic dysregulation. Two tryptophan pathway metabolites, indole-3-acetaldehyde and N-Acetyl-5-hydroxytryptamine were decreased after irradiation, whereas these metabolites showed a pronounced recovery in mice receiving FMT. Proteomics analysis of small intestine indicated that radiation enteritis triggered immune-inflammatory responses, which were potentially mitigated by FMT. In 21 patients receiving pelvic radiotherapy for cervical cancer, those who developed enteritis (n = 15) had higher abundance in Lachnospiraceae. Moreover, Indole-3-acetaldehyde was reduced after irradiation. These findings provide insights into the therapeutic effects of FMT in radiation enteritis and highlight Lachnospiraceae and the tryptophan metabolite, Indole-3-acetaldehyde may protect against radiation enteritis.<\/jats:p>","DOI":"10.1667\/rade-23-00189.1","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T22:53:26Z","timestamp":1716850406000},"page":"572-585","source":"Crossref","is-referenced-by-count":16,"title":["Fecal Microbiota Transplantation Repairs Radiation Enteritis Through Modulating the Gut Microbiota-Mediated Tryptophan Metabolism"],"prefix":"10.1667","volume":"201","author":[{"given":"Yeqiang","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lumeng","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"34","doi-asserted-by":"crossref","unstructured":"CA Smith, EJ Want, G O'Maille, et al.  \"Anal Chem.\"  78, \"XCMS: Processing mass spectrometry data for metabolite profiling using Nonlinear peak alignment, matching, and identification.\" 779 (2006)","DOI":"10.1021\/ac051437y"},{"key":"7","doi-asserted-by":"crossref","unstructured":"T Kumagai, F Rahman, AM Smith,  \"Nutrients.\"  10, \"The microbiome and radiation induced-bowel injury: Evidence for potential mechanistic role in disease pathogenesis.\" (2018)","DOI":"10.3390\/nu10101405"},{"key":"6","doi-asserted-by":"crossref","unstructured":"AL Kau, PP Ahern, NW Griffin, et al.  \"Nature.\"  474, \"Human nutrition, the gut microbiome and the immune system.\" 327 (2011)","DOI":"10.1038\/nature10213"},{"key":"50","doi-asserted-by":"crossref","unstructured":"Y Li, H Yan, Y Zhang, et al.  \"Front Cell Infect Microbiol.\"  10, \"Alterations 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